, Volume 89, Issue 3, pp 169–175 | Cite as

Antifungal activity of ovotransferrin towards genus Candida

  • Piera Valenti
  • Paolo Visca
  • Giovanni Antonini
  • Nicola Orsi


The inhibiting activity of ovotransferrin was tested towards different species belonging to genus Candida.

Of one hundred strains tested, only C. krusei showed a noticeable resistance, while the other species appeared to be more sensitive than bacteria to the action of ovotransferrin. The influence of anions, such as bicarbonate and citrate, on the inhibiting activity of ovotransferrin was also investigated. Moreover it was observed that iron saturated ovotransferrin retained its activity, thus suggesting an interaction between the protein and Candida cells.


Iron Citrate Bicarbonate Inhibiting Activity Candida 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Antonini, E., N. Orsi & P. Valenti, 1977. Effetto delle transferrine sulla patogenicità delle Enterobacteriaceae. G. Mal. Infet. Parassit. 29: 481–489.Google Scholar
  2. 2.
    Atkin, C. L., J. B. Neilands & H. J. Phaff, 1970. Rhodotorulic acid from species of Leucosporidium, Rhodosporidium, Sporidiobolus and Sporobolomyces and a new alanine-containing ferrichrome from Cryptococcus melibiosum. J. Bacteriol. 103: 722–733.Google Scholar
  3. 3.
    Byers, B. B. & J. E. Arcenaux, 1977. Microbial transport and utilization of iron. In: E. D. Weimberg (ed.) Microorganisms and Minerals. Marcel Dakker, New York: 215–249.Google Scholar
  4. 4.
    Caroline, L., F. Rosner & P. J. Kozinn, 1969. Elevated se- rum iron, low unbound transferrin and candidiasis in acute leukemia. Blood 34: 441–451.Google Scholar
  5. 5.
    Chilgren, R. A., R. Hong & P. G. Quie, 1968. Human serum interactions with Candida albicans. J. Immunol. 101: 128–132.Google Scholar
  6. 6.
    Emery, T., 1974. Biosynthesis and mechanism of action of hydroxamate type siderochromes. In: J. B. Neilands (ed.) Microbial Iron Metabolism. Academic Press Inc., New York: 107–123.Google Scholar
  7. 7.
    Frost, G. E. & H. Rosemberg, 1973. The citrate-dependent iron transport system in Escherichia coli K-12. Biochim. Biophys. Acta 330: 90.Google Scholar
  8. 8.
    Garcia-Mendoza, C. & M. Movales-Ledieu, 1968. Chitin in the new wall of regenerating protoplasts of Candida utilis. Nature London 220: 1035.Google Scholar
  9. 9.
    Hoizberg, M. & W. M. Artis, 1983. Hydroxamate siderophore production by opportunistic and systemic fungal pathogens. Infect. Immun. 40: 1134–1139.Google Scholar
  10. 10.
    Mackenzie, D. W. R., 1962. Serum tube identification of Candida albicans. J. Clin. Pathol. 15: 563–565.Google Scholar
  11. 11.
    Neilands, J. B., 1980. Microbial metabolism of iron. In: A. Jacobs & M. Worwood (eds.) Iron in Biochemistry and Medicine, II. Academic Press Inc., London: 529–572.Google Scholar
  12. 12.
    Phelps, C. & E. Antonini, 1975. A study on the kinetics of iron and copper binding to hen ovotransferrin. Biochem. J. 147: 385–391.Google Scholar
  13. 13.
    Reinhardt, J. H., A. M. Allen, D. Gunnison & W. A. Akers, 1974. Experimental human Trichophyton mentagrophytes infections. J. Invest. Dermatol. 63: 419–422.Google Scholar
  14. 14.
    Shiraishi, A. & T. Arai, 1979. Antifungal activity of transferrin. Sabouraudia 17: 79–83.Google Scholar
  15. 15.
    Silva Hutner, M. & B. H. Cooper, 1980. Yeasts of medical importance. In: E. H. Lanette, A. Balows, W. J. Hausler Jr. & J. P. Truant (eds.) Manual of Clinical Microbiology, American Society for Microbiology, Washington D.C. Third edition: 562–576.Google Scholar
  16. 16.
    Simonetti, N., V. Strippoli & A. Cassone, 1974. Yeastmycelial conversion induced by N-acetyl-D-glucosamine in Candida albicans. Nature (London) 250: 344–346.Google Scholar
  17. 17.
    Valenti, P., A. De Stasio, L. Seganti, P. Mastromarino, L. Sinibaldi & N. Orsi, 1980. Capacity of Staphylococci to grow in the presence of ovotransferrin or CrCl3 as a character of potential pathogenicity. J. Clin. Microbiol. 11: 445–447.Google Scholar
  18. 18.
    Valenti, P., A. De Stasio, P. Mastromarino, L. Seganti, L. Sinibaldi & N. Orsi, 1981. Influence of bicarbonate and citrate on the bacteriostatic action of ovotransferrin. FEMS Microbiol. Lett. 10: 77–79.Google Scholar
  19. 19.
    Valenti, P., M. Guarino, P. Visca, C. von Hunolstein, G. Antonini, A. De Stasio & N. Orsi, 1981. Resistance of genus Proteus to ovotransferrin. Boll. Ist. Sieroter. Milanese 60: 284–287.Google Scholar
  20. 20.
    Valenti, P., G. Antonini, M. R. Rossi Fanelli, N. Orsi & E. Antonini, 1982. Antibacterial activity of matrix-bound ovotransferrin. Antimicrob. Agents Chemother. 21: 840–841.Google Scholar
  21. 21.
    Valenti, P., C. von Hunolstein, P. Visca, G. Antonini, N. Orsi & E. Antonini, 1982. Studies on the antibacterial activity of ovotransferrin. In: P. Saltman & J. Hegenauer (eds.) The Biochemistry and Physiology of Iron. Elsevier Biomedical, New York, Amsterdam, Oxford: 725–726.Google Scholar
  22. 22.
    Valenti, P., Antonini, G., C. von Hunolstein, P. Visca, N. Orsi & E. Antonini, 1983. Studies on the antimicrobial activity of ovotransferrin. Int. J. Tiss. Reac. 1: 97–105.Google Scholar
  23. 23.
    Weimberg, E. D., 1971. Role of iron in host-parasite interactions. J. Infect. Dis. 124: 401–410.Google Scholar
  24. 24.
    Young, I. G., G. B. Cox & F. Gibson, 1967. 2,3-Dihydroxybenzoate as bacterial growth factor and its route of biosynthesis. Biochim. Biophys. Acta 441: 319–331.Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1985

Authors and Affiliations

  • Piera Valenti
    • 1
  • Paolo Visca
    • 1
  • Giovanni Antonini
    • 1
  • Nicola Orsi
    • 1
  1. 1.Istituto di Microbiologia, Facoltà di Medicina e ChirurgiaUniversità di Roma ‘La Sapienza’RomaItalia

Personalised recommendations