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Function of laccase in the white-rot fungus Fomes annosus

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Abstract

  1. 1.

    Thioglycolic acid, a Cu-chelating agent, totally inhibited extracellular laccase activity without affecting growth and morphology of Fomes annosus.

  2. 2.

    In the presence of thioglycolic acid Fomes annosus cleaved high molecular weight lignosulfonate with a molecular weight range of 2×106 to 1000. In the absence of thioglycolic acid the polymerizing activity of laccase prevented the detection of lignosulfonate breakdown products.

  3. 3.

    Oxidative polymerization of a lignin monomer, coniferyl alcohol, occurred in the presence but not in the absence of laccase activity.

  4. 4.

    Catechol and guaiacol added to the medium at a concentration of 2 mmol, are normally oxidized by fungal laccase and strongly inhibit growth. Presence of thioglycolic acid prevented the oxidation of these phenols and simultaneously permitted normal growth.

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Abbreviations

HM-LS:

high molecular lignosulfonate

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Correspondence to A. Haars.

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Haars, A., Hüttermann, A. Function of laccase in the white-rot fungus Fomes annosus . Arch. Microbiol. 125, 233–237 (1980). https://doi.org/10.1007/BF00446882

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Key words

  • Laccase
  • Thioglycolic acid
  • Lignin degradation
  • Polymerization
  • Coniferyl alcohol
  • Catechol
  • Fomes annosus