Archives of Microbiology

, Volume 125, Issue 3, pp 221–225

Lindane degradation by cell-free extracts of Clostridium rectum

  • N. Ohisa
  • M. Yamaguchi
  • N. Kurihara
Article

Abstract

For lindane degradation, a cell suspension of Clostridium rectum strain S-17 demands the addition of substrates such as leucine, alanine, pyruvate, a leucine-proline mixture, and molecular hydrogen. In the presence of leucine-proline mixture, lindane decomposed in parallel with isovaleric acid formation, and both lindane degradation and isovaleric acid formation were inhibited by monoiodoacetic acid, suggesting a close relation between lindane degradation and the Stickland reaction. Lindane was degraded by cell-free extracts of C. rectum in the presence of dithiothreitol (DTT). Radiogaschromatograms of n-hexane soluble metabolites from [14C] lindane showed the presence of monochlorobenzene and γ-3,4,5,6-tetrachlorocyclohexene, Leucine, NADH, and NADPH were somewhat less active than DTT for lindane degradation in cell-free extracts. Reductive dechlorination seemed the major route of lindane degradation in cell-free extracts as well as in the intact cells of C. rectum.

Key words

Clostridium rectum Lindane degradation Stickland reaction Monochlorobenzene Tetrachlorocyclohexene Reductive dechlorination 

Abbreviations

Lindane (γ-HCH)

γ-1,2,3,4,5,6-hexachlorocyclohexane

α-HCH

α-1,2,3,4,5,6-hexachlorocyclohexane

γ-TCH

γ-3,4,5,6-tetrachlorocyclohexene

γ-PCH

γ-1,3,4,5,6-pentachlorocyclohexene

DTT

1,4-dithiothreitol

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • N. Ohisa
    • 1
  • M. Yamaguchi
    • 1
  • N. Kurihara
    • 2
  1. 1.Department of Agricultural ChemistryUniversity of Osaka PrefectureSakai, OsakaJapan
  2. 2.Radioisotope Research CenterKyoto UniversityKyotoJapan

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