Archives of Microbiology

, Volume 123, Issue 2, pp 209–212

N-Glycyl-glucosamine: A novel constituent in the cell wall of Halococcus morrhuae

  • Josef Steber
  • Karl Heinz Schleifer
Article

Abstract

The dinitrophenyl-derivative of N-glycylglucosamine was isolated from partially hydrolyzed dinitrophenylated cell walls of Halococcus morrhuae CCM 859. To increase the yield of amino-terminal glycine residues, halococcal cell walls were treated with alkali or acid prior to dinitrophenylation. Authentic N-glycyl-glucosamine was used as a reference substance. A substitution of the amino group of glucosamine by an amino acid has so far not been found in any other wall of a pro- or eucaryotic cell. Since only 5% of the glycine residues reveal an unsubstituted carboxyl group within intact cell walls, glycine may play a role in connecting glycan strands through peptidic linkages between the amino group of glucosamine and the carboxyl group of an uronic acid or gulosaminuronic acid.

Key words

N-Glycyl-glucosamine Cell wall Hatococcus Archaebacteria 

Abbreviations

DNP

dinitrophenyl

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Josef Steber
    • 1
  • Karl Heinz Schleifer
    • 2
  1. 1.Lehrstuhl für Mikrobiologie der Universität MünchenMünchen 19Germany
  2. 2.Lehrstuhl für Mikrobiologie der Technischen Universität MünchenMünchen 2Germany
  3. 3.Henkel KGaADusseldorf 1Germany

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