Archives of Microbiology

, Volume 123, Issue 2, pp 137–141 | Cite as

Amino acid utilization patterns in clostridial taxonomy

  • Sidney R. Elsden
  • Martin G. Hilton
Article

Abstract

The polyamide layer technique for the chromatographic separation of dimethylaminonaphthalene sulphonyl amino acids has been adapted to the qualitative analysis of amino acids in media before and after the growth of micro-organisms. The method has been used to study the amino acids metabolized by cultures of proteolytic clostridia growing in a medium consisting of an acid hydrolysate of casein as a source of amino acids and small amounts of yeast extract and trypticase as sources of growth factors. The chromatograms of the media after growth showed which amino acids were used and which new amino acids were produced.

Clostridium botulinum type F (proteolytic), C. ghoni, C. mangenoti and C. putrificum were found to reduce proline to 5-aminovaleric acid and to produce 2-aminobutyric acid, properties they shared with C. sporogenes and C. sticklandii. C. botulinum type G and C. subterminale used glycine, lysine, serine, and arginine but in contrast to C. sticklandii they neither reduced proline to 5-aminovaleric acid nor produced 2-aminobutyric acid. Both organisms oxidized phenylalanine, tyrosine and tryptophan to phenylacetic acid, p-hydroxyphenyl acetic acid and indole acetic acid respectively. C. lituseburense and C. scatologenes used serine, threonine and arginine and produced 2-amino butyric acid and ornithine. C. lentoputrescens, C. limosum and C. malenomenatum resembled C. tetanomorphum by using glutamic acid and tyrosine. The chromatograms always showed the physiological group to which an organism belonged and in some cases were characteristic of the species.

Key words

2-aminobutyric acid 5-aminovaleric acid Glutamic acid Lysine Proline Tyrosine Polyamide layer Chromatography Clostridia 

Abbreviations

Abu

2-aminobutyric acid

Ava

5-aminovaleric acid

DNS

1-dimethyaminonaphthalene-5-sulphonyl

DNS-Cl

the sulphonyl chloride

DNS-NH2

the sulphonamide

DNS-OH

the sulphonic acid

VFA

steam volatile fatty acid

u

unknown

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Sidney R. Elsden
    • 1
  • Martin G. Hilton
    • 1
  1. 1.Institute Colney LaneAgricultural Research Council Food ResearchNorwichU.K.

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