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Different degradation pathways for glucose and fructose in Rhodopseudomonas capsulata


In Rhodopseudomonas capsulata the enzymes of the Entner-Doudoroff pathway and the Embden-Meyerhof pathway have been examined. Fructose-grown cells contained inducible activities of phosphoenolpyruvate-fructosephospho-transferase and 1-phosphofructokinase and only low levels of fructokinase and 6-phosphofructokinase. Although fructose-grown cells contained, in addition, all the enzymes of the Entner-Doudoroff pathway together with fructose-1,6-diphosphatase and phosphoglucose isomerase, the Entner-Doudoroff pathway was not operative in fructose catabolism and served only the degradation of glucose. The functional separation of glucose and fructose catabolism via the Entner-Doudoroff and a modified Embden-Meyerhof pathway, respectively, was confirmed by different approaches:

  1. 1.

    Radiorespirometric experiments with glucose and fructose labelled in positions 1, 2, 3+4 and 6 have been carried out. The pattern of 14CO2-evolution from position-labelled glucose was characteristic for the Entner-Doudoroff pathway, that from position-labelled fructose for the Embden-Meyerhof pathway.

  2. 2.

    In the presence of arsenite up to 50% of glucose-and fructose-carbon was excreted as pyruvate. Using 1-14C-glucose, 86% of the pyruvate was labelled in the carboxyl group, whereas using 1-14C-fructose only 19% of the pyruvate was labelled in the carboxyl group.

  3. 3.

    A glucose-6-phosphate dehydrogenase-deficient mutant was isolated which lacked a functional Entner-Doudoroff pathway but which was unaltered in its ability to grow on fructose.

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Embden-Meyerhof pathway


F.ntner-Doudoroff pathway


pentose-phosphate pathway








phosphoglucose isomerase






















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Conrad, R., Schlegel, H.G. Different degradation pathways for glucose and fructose in Rhodopseudomonas capsulata . Arch. Microbiol. 112, 39–48 (1977).

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Key words

  • Rhodopseudomonas capsulata
  • Hexose catabolism
  • Entner-Doudoroff pathway
  • Embden-Meyerhof pathway
  • PEP-fructose-phosphotransferase
  • Mutant isolation
  • Radiometric techniques