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A note on disc-electrophoresis of human haptoglobin in acrylamide gel

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Four techniques for the electrophoretic separation of human haptoglobins have been experimented. The results are compared, and the conclusion drawn that disc-electrophoresis with 5.5% acrylamide (Cyanogum), ammonium persulphate as gelling catalyst, and TRIS-glycine buffer for the electrode compartments, gives the optimal separation of Hp polymers.


Vier Techniken für die elektrophoretische Trennung der menschlichen Haptoglobine wurden erprobt. Die Resultate werden verglichen und der Schluß gezogen, daß Disc-Electrophoresis mit 5,5% Acrylamide (Cyanogum), Ammoniumpersulphate als Katalysator und TRIS-Glycine-Puffersystem für die Elektrodenfächer die beste Trennung der Hp-Polymere gibt.

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  1. Bernini, L. F., Borri-Voltattorni, C.: Studies con the structure of human haptoglobins. I Spontaneous refolding after extensive reduction and dissociation. Biochim. biophys. Acta (Amst.) 200, 203–219 (1970).

  2. Cleve, H., Herzog, P.: Phenotype variations of haptoglobin Johnson types. Humangenetik 7, 218–224 (1969)

  3. Holmes, R.: Discontinous acrylamide-gel plate electrophoresis. Biochim. biophys. Acta (Amst.) 133, 174–176 (1967).

  4. Margolis, J., Kenrick, K. G.: Two dimensional resolution of Plasma proteins by combination of Polyacrylamide disc. Nature (Lond.) 221, 1056–1057 (1969).

  5. Peacock, A. C., Bunting, L. S., Quenn, K. G.: Serum protein electrophoresis in acrylamide gel: patterns from normal human subjects. Science 147, 1451–1453 (1965).

  6. Ritchie, R. F., Harter, J. G., Bayles, T. B.: Refinements of acrylamide electrophoresis. J. Lab. clin. Med. 68, 842–850 (1966).

  7. Serafini, N. A.: Nota sull'appicazione della tecnica dell'elettroforesis a disco in gel di acrilamide per l'identificazione dei fenotipi aptoglobinici. Acta med. Romana 4, 448–453 (1966).

  8. Sheridan, J. W., Kenrick, K. G., Margolis, J.: Phenotypic expression of the Hp2/Hp1 genotype. Biochem. biophys. Res. Commun. 35, 474–477 (1969).

  9. Woodworth, R. C., Clarck, G. G.: An improved vertical polyacrylamide gel electrophoresis apparatus. Application to typing and subtyping of haptoglobins. Analyt. Biochem. 18, 295–305 (1967).

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This work has been supported in part by grant 115. 1839. 04817 from CNR.

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Crosti, N., Rizzato, L. A note on disc-electrophoresis of human haptoglobin in acrylamide gel. Hum Genet 13, 66–68 (1971).

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  • Polymer
  • Ammonium
  • Internal Medicine
  • Metabolic Disease
  • Acrylamide