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Current Genetics

, Volume 2, Issue 1, pp 69–74 | Cite as

Chromosomal proteins in Saccharomyces cerevisiae

I. number and properties of individual proteins
  • Kay Gulløv
  • Jørgen Friis
Article

Summary

Proteins were isolated from purified yeast chromatin and subjected to two-dimensional electrophoresis. The cellular and the chromosomal content of the major nonhistone proteins was measured. Two polypeptides of molecular weights 55,000 and 53,000, identified as α and β tubulin, and a polypeptide of molecular weight 63,000, associated with the nuclear DNA to a very high degree, account for nearly 50% of the nonhistone proteins present in chromatin. Only one tenth of the RNA polymerase subunit with the molecular weight of 23,000 was associated with nuclear DNA following chromatin purification in metrizamide gradients.

Key words

Metrizamide Nonhistone proteins Two-dimensional electrophoresis Tubulin 

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Kay Gulløv
    • 1
  • Jørgen Friis
    • 1
  1. 1.Department of Molecular BiologyUniversity of OdenseOdense MDenmark

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