Odc-rs5 was previously identified as a complex locus closely linked to the Igk complex on mouse Chromosome (Chr) 6 and comprising at least five copies of a sequence related to the mRNA encoding ornithine decarboxylase (ODC) in the genomes of mice of some inbred strains and at least seven copies in others (Richards-Smith and Elliott, Mammalian Genome 2: 215, 1992). In the present study, Odc-rs5 was shown to be composed of at least seven copies of the ODC sequence in both the Odc-rs5 a and Odc-rs5 b haplotypes. Based upon the distribution of DNA restriction fragments (RFs) that had previously been associated with Odc-rs5 a or Odc-rs5 b among 42 mice of inbred laboratory strains having various haplotypes at Igk and in mice of two congenic strains [B6.PL-Ly-2 a, Ly-3 a(75NS)/Cy and B6.PL-Ly-2 a, Ly-3 a(85NS)/Cy] and a backcross-derived stock (NAK) known to be recombinant within Igk, a fine structure map of Odc-rs5 was deduced relative to Igk and more distal loci. Odc-rs5-derived RFs were located to three distinct regions within and/or distal to Igk and to a fourth site between (Ly-3, Ly-2) and Raf-1. Additionally, DNAs from 19 mice of inbred strains and random-bred stocks derived from wild progenitors trapped at various locations were analyzed and found to exhibit an unexpected variety of combinations of RFs associated with the two Odc-rs5 haplotypes most frequently observed among inbred laboratory strains of mice.
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Berger, F.G.: Assignment of a gene encoding ornithine decarboxylase to the proximal region of chromosome 12 in the mouse. Biochem Genet 27: 745–753, 1989.
Berger, F.G., Szymanski, P., Read, E., and Watson, G.: Androgen regulated ornithine decarboxylase mRNAs of mouse kidney. J Biol Chem 259: 7941–7946, 1984.
Boyd, R.T., Goldrick, M.M., and Gottlieb, P.D.: Genetic polymorphism at the mouse immunoglobulin J k locus (Igk-J) as demonstrated by Southern hybridization and nucleotide sequence analysis. Immunogenetics 24: 150–157, 1986.
Cox, D.R., Trouillot, T., Ashley, P.L., Brabant, M., and Coffino, P.: A functional mouse ornithine decarboxylase gene (Odc) maps to chromosome 12: Further evidence of homology between mouse chromosome 12 and the short arm of human chromosome 2. Cytogenet Cell Genet 48: 92–94, 1988.
D'Hoostelaere, L.A., Huppi, K., Mock, B., Mallett, C., Gibson, D., Hilgers, J., and Potter, M.: The organization of the immunoglobulin kappa locus in mice. Curr Top Microbiol Immunol 137: 116–129, 1988a.
D'Hoostelaere, L.A., Huppi, K., Mock, B., Mallett, C., and Potter, M.: The Igk L chain allelic groups among the Igk haplotypes and Igk crossover populations suggest a gene order. J Immunol 141: 652–661, 1988b.
Elliott, R.W., Sparkes, R.S., Mohandas, T., Grant, S.G., and McGinnis, J.F.: Localization of the rhodopsin gene to the distal half of mouse chromosome 6. Genomics 6: 635–644, 1990.
Gibson, D.M., Maclean, S.J., and Cherry, M.: Recombination between kappa chain genetic markers and the Lyt-3 locus. Immunogenetics 18: 111–116, 1983.
Gibson, D.M., Maclean, S.J., Anctil, D., and Mathieson, B.J.: Recombination between kappa chain genetic markers in the mouse. Immunogenetics 20: 493–501, 1984.
Gorman, S.D., Sun, Y.H., Zamoyska, R., and Parnes, J.R.: Molecular linkage of the Ly-3 and Ly-2 genes. Requirement of Ly-2 for Ly-3 surface expression. J Immunol 140: 3646–3653, 1988.
Gupta, M. and Coffino, P.: Mouse ornithine decarboxylase: complete amino acid sequence deduced from cDNA. J Biol Chem 260: 2941–2944, 1985.
Jänne, O.A., Kontula, K.K., Isomaa, V.V., and Bardin, C.W.: Ornithine decarboxylase mRNA in mouse kidney: a low abundancy gene product regulated by androgens with rapid kinetics. Ann NY Acad Sci 438: 72–84, 1984.
Katz, A. and Kahana, C.: Isolation and characterization of the mouse ornithine decarboxylase gene. J Biol Chem 263: 7604–7609, 1988.
Kelley, D.E. and Perry, R.P.: Association of an ornithine decarboxylase processed pseudogene with members of a Vk immunoglobulin gene family provides a useful evolutionary clock. Nucleic Acids Res 15: 7199, 1987.
Kofler, R., Duchosal, M.A., and Dixon, F.J.: Complexity, polymorphism, and connectivity of mouse Vk gene families. Immunogenetics 29: 65–74, 1989.
Kontula, K.K., Torkkeli, T.K., Bardin, C.W., and Jänne, O.A.: Androgen induction of ornithine decarboxylase mRNA in mouse kidney as studied by complementary DNA. Proc Natl Acad Sci USA 81: 731–735, 1984.
Malissen, M., McCoy, C., Blane, D., Trucy, J., Devaux, C., Schmitt-Verhulst, A.-M., Fitch, F., Hood, L., and Malissen, B.: Direct evidence for chromosomal inversion during T-cell receptor β-gene rearrangements. Nature 319: 28–33, 1986.
Potter, M., Newell, J.B., Rudikoff, S., and Haber, E.: Classification of mouse VK groups based on the partial amino acid sequence to the first invariant tryptophan: Impact of 14 new sequences from IgG myeloma proteins. Mol Immunol 19: 1619–1630, 1982.
Richards-Smith, B. and Elliott, R.W.: Mapping of a family of repeated sequences in the mouse. Mouse News Lett 71: 46, 1984.
Richards-Smith, B.A. and Elliott, R.W.: Mapping of the mouse ornithine decarboxylase-related sequence family. Mammalian Genome 2: 215–232, 1992.
Roderick, T.J. and Guidi, J.N.: Strain distribution of polymorphic variants. In M.F. Lyon and A.G. Searle (eds.); Genetic Variants and Strains of the Laboratory Mouse, 2nd ed., pp. 663–772, Oxford University Press, New York, 1989.
Siracusa, L.D., Jenkins, N.A., and Copeland, N.G.: Identification and applications of repetitive probes for gene mapping in the mouse. Genetics 127: 169–179, 1991.
Stephenson, D.A., Elliott, R.W., Chapman, V.M., and Grant, S.G.: Identification of an X-linked member of the Odc gene family in the mouse. Nucleic Acids Res 16: 1642–1643, 1988.
Strohal, R., Helmberg, A., Kroemer, G., and Kofler, R.: Mouse Vk gene classification by nucleic acid sequence similarity. Immunogenetics 30: 475–493, 1989.
Taylor, B.A., Rowe, L., Gibson, D.M., Riblet, R., Yetter, R., and Gottlieb, P.D.: Linkage of a 7S RNA sequence and kappa light chain genes in the mouse. Immunogenetics 22: 471–481, 1985.
Villani, V., Coffino, P., and D'Eustachio, P.: Linkage genetics of mouse ornithine decarboxylase. Genomics 5: 636–638, 1989.
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Richards-Smith, B.A., Elliott, R.W. Fine-structure mapping of the complex locus Odc-rs5 relative to Igk and distal loci. Mammalian Genome 3, 689–699 (1992). https://doi.org/10.1007/BF00444364
- Fine Structure
- Restriction Fragment
- Distinct Region
- Inbred Strain