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Mycopathologia

, Volume 92, Issue 2, pp 93–99 | Cite as

Experimental pulmonary candidiasis in modified rabbits

II. Immunohistochemical evidence of participation of immune complexes in the formation of fungal lesions in C. albicans-sensitized hosts
  • Toshitsugu Nakamura
Article

Abstract

Pulmonary lesions induced by an intratracheal inoculation of Candida albicans into rabbits in untreated control, bovine serum albumin (BSA)-sensitized and C. albicans-sensitized groups were examined immunohistochemically to clarify the localization of IgG, IgM and C3. In the control group no inflammatory cells were immunoreactive for IgG and only a few macrophages for IgM and C3, whereas in the BSA- and C. albicans-sensitized groups there were a small number of IgG-positive polymorphonuclear leukocytes and IgM- and C3-positive macrophages in the lesions, the latter group being more prominent. Furthermore, epithelioid granulomatous lesions at the late stage in the C. albicans-sensitized animals showed scattered epithelioid cells containing IgG as well as abundant IgG- and IgM-positive plasma cells. These immunohistochemical results were considered to support the estimation that immune complexes contributed to the modification of fungal lesions in the C. albicans-sensitized hosts, although non-immunological defense mechanisms seemed to be more important in the elimination of the fungus.

Keywords

Bovine Serum Albumin Candida Plasma Cell Untreated Control Defense Mechanism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Baker, R. D., 1947. Tissue changes in fungas disease. Arch. Pathol. 44: 459–466.Google Scholar
  2. 2.
    Emori, K. & A. Tanaka, 1978. Granuloma formation by synthetic bacterial cell wall fragment: muramyl dipeptide. Infect. Immun. 19: 613–620.Google Scholar
  3. 3.
    Fukazawa, Y., T. Shinoda, K. Kagaya & A. Nishikawa, 1976. Immune response and defense mechanism in experimental candidiasis in mice. Jpn. J. Med. Mycol. 17: 64–68.Google Scholar
  4. 4.
    Hsu, S-M., L. Raine & H. Fanger, 1981. Use of avidinbiotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29: 577–580.Google Scholar
  5. 5.
    Jones, J. M., 1980. Quantitation of antibody against cell wall mannan and a major cytoplasmic antigen of Candida in rabbits, mice, and humans. Infect. Immun. 30: 78–89.Google Scholar
  6. 6.
    Kawaoi, A., 1983. Illustrated Technology of Immunofluorescence: its principles, practice and applications. Soft Science Inc., Tokyo: 36.Google Scholar
  7. 7.
    Lange, S., H. Nygen, J-E. Brorson, I. Holmberg & P. Larsson, 1981. Diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA) for detection of class-specific antibodies to Aspergillus fumigatus and Candida albicans. Acta Pathol. Microbiol. Scand. Sect. C. 89: 387–389.Google Scholar
  8. 8.
    Meister, H., B. Heymer, H. Schäfer & O. Haferkamp, 1977. Role of Candida albicans in granulomatous tissue reactions. II. In vitro degradation of C. albicans in hepatic macrophages in mice. J. Infect. Dis. 135: 235–242.Google Scholar
  9. 9.
    Nakamura, T., 1984. Experimental pulmonary candidiasis in modified rabbits. Histopathological, ultrastructural and enzyme cytochemical studies of tissue reactions. Mycopathologia 85: 129–144.Google Scholar
  10. 10.
    Nakane, P. K. & G. B. Pierce, 1967. Enzyme-labeled antibody: preparation and application for the localization of antigens. J. Histochem. Cytochem. 14: 929–931.Google Scholar
  11. 11.
    Potter, M., 1981. Immunoglobulins M, G, and A. In: J. J. Oppenheim, D. L. Rosenstreich & M. Potter (eds), Cellular functions in immunity and inflammation. Edward Arnold, London: 229–258.Google Scholar
  12. 12.
    Ridley, M. J., Y. Marianayagam & W. G. Spector, 1982. Experimental granulomas induced by mycobacterial immune complexes in rats. J. Pathol. 136: 59–72.Google Scholar
  13. 13.
    Ridley, M. J. & D. Russell, 1982. An immunoperoxidase study of immunological factors in high immune and low resistance granulomas in leprosy. J. Pathol. 137: 149–157.Google Scholar
  14. 14.
    Sandberg, A. L., 1981. Complement. In: J. J. Oppenheim, D. L. Rosenstreich & M. Potter (eds), Cellular functions in immunity and inflammation. Edward Arnold, London: 373–395.Google Scholar
  15. 15.
    Spector, W. G. & N. Heesom, 1969. The production of granulomata by antigen-antibody complexes. J. Pathol. 98: 31–39.Google Scholar
  16. 16.
    Tada, T. & K. Ishizuka, 1966. Arthus type inflammation with rabbit γM antibody. J. Immunol. 96: 112–118.Google Scholar
  17. 17.
    Warren, K. S., 1976. A functional classification of granulomatous inflammation. Ann. N. Y. Acad. Sci. 278: 7–18.Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1985

Authors and Affiliations

  • Toshitsugu Nakamura
    • 1
  1. 1.Department of PathologyShinshu University, School of MedicineMatsumotoJapan

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