, Volume 119, Issue 2, pp 115–125 | Cite as

Light and electron microscopic studies on experimental nocardia-toxicosis in mice

  • Kiyoshi Terao
  • Emiko Ito
  • Motoko Oarada
  • Misako Ohkusu
  • Katsukiyo Yazawa
  • Yuzuru Mikami
  • Kazuei Igarashi
Mycotoxicoses and Mycotoxins


An exotoxin (HS-6) produced by Nocardia otitidiscaviarum isolated from certain lesions of cutaneous nocardiosis of a male 82-year-old patient induced severe injuries in the pancreas, liver, stomach, small intestine, heart, thymus and kidney of male ICR mice. Mice given Nocardia-free preparation of HS-6 at a dose of 1 mg/kg of body weight developed several autophagic vacuoles in the pancreas and liver within 20 min after the i.p. injection. Thereafter, the autophagic vacuoles increased in number and size with time. About 24 hr after the administration of HS-6, the liver showed marked accumulation of fat droplets in the cytoplasm of the hepatocytes. Although they contained abundant autophagic vacuoles in the regions of RER, there were no lipomatoses in the acinar cells of the pancreas, those of the chief cells and smooth muscle cells of the stomach, Paneth cells, goblet cells, smooth muscle cells of the small intestine, and plasma cells in the digestive tract. Biochemical examinations revealed that HS-6 had no significant effect on the protein synthesis of reticulocytes. Inoculation of the Nocardia into the mouse peritoneal cavities caused marked granulomatoses in the pancreas, liver and regional lymph nodes, but did not develop autophagic vacuoles in RER regions of these organs.

Key words

Nocardia otitidiscaviarum exotoxin poisoning RER autophagic vacuole nocardiosis fatty liver 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Beaman BL. Actinomycetes pathogenesis. In: M Goodfellow, M Mordarski, ST Williams (eds.), The biology of the actinomycetes. New York: Academic Press, 1984: 457–479.Google Scholar
  2. 2.
    Arroyo JC, Nichols S, Carroll GF. Disseminated nocardia caviae infection. Am J Med 1977; 62: 409–412.Google Scholar
  3. 3.
    Simpson GL, Stinson EB, Egger MJ, Remington JS. Nocardial infections in the immunocompromised host: A detailed study in a defined population. Rev Inf Disease 1981: 492–507.Google Scholar
  4. 4.
    Smego RA, Moeller MB, Gallis HA. Trimethoprinsulfamethoxazole therapy for Nocardia infections. Arch Intern Med 1983; 143: 711–718.Google Scholar
  5. 5.
    Miami Y, Yazawa K. Susceptibility patterns of pathogenic nocardia to some selected antimicrobial agents and their usefulness in the identification work in a clinical laboratory. Bull JFCC 1989; 5: 89–95.Google Scholar
  6. 6.
    Mikami Y, Yu SF, Yazawa K, Fukushima K, Maeda A, Uno J, Terao K, Saito M, Kubo A, Suzuki K. A toxic substance produced by Nocardia otitidiscaviarum isolated from cutaneous nocardiosis. Mycopathologia 1990; 112: 113–118.Google Scholar
  7. 7.
    Ogasawara T, Ito K, Igarashi K. Effect of polyamines on globin synthesis in a rabbit reticulocyte polyamine-free protein synthesis system. J Biochem 1989; 105: 154–167.Google Scholar
  8. 8.
    Ohkawa H, Ohishi N, Yagi K. Assay for peroxides in animal tissues by thiobarbituric acid reaction. Analyt Biochem 1979; 95: 351–358.Google Scholar
  9. 9.
    Ashford TP, Porter KR. Cytoplasmic components in hepatic cell lysosome. J Cell Biol 1962; 12: 198–202.Google Scholar
  10. 10.
    Novikoff AB, Shin W. The endoplasmic reticulum in the Golgi zone and its relations to microbodies, Golgi apparatus, and autophagic vacuoles in rat liver cells. J Microscop 1964; 3: 187–206.Google Scholar
  11. 11.
    Butler WH. Further ultrastructural observations on injury of rat hepatic parenchymal cells induced by aflatoxin B1. Chem-Biol Interactions 1971/72; 4: 49–65.Google Scholar
  12. 12.
    Terao K, Ito E, Oarada M, Murata M, Yasumoto T. Histopathological studies on experimental marine toxin poisoning. 5. The effects in mice of yessotoxin isolated from Patinopecten yessoensis and of a desulfated derivative. Toxicon 1990; 28: 1095–1104.Google Scholar
  13. 13.
    Arstila AU, Trump BF. Studies on cellular autophagocytosis. Am J Path 1968; 53: 687–733.Google Scholar
  14. 14.
    Rumpelt HJ, Weisbach. Effect of cyclohexamide on glucagon-induced autophagy. Am J Path 1978; 91: 49–55.Google Scholar
  15. 15.
    Hruban Z, Spargo B, Swift H, Wissler RW, Kleifeld RG. Focal cytoplasmic degradation. Am J Path 1963; 42: 657–683.Google Scholar
  16. 16.
    Verbin RS, Difuiso G, Farber E. Protective effects of cyclohexamide against 1-β-D-arabinosylcytosine-induced intestinal lesions. Cancer Res 1973; 33: 2086–2093.Google Scholar
  17. 17.
    Oarada M, Ito E, Mikami Y, Yazawa K, Fujimoto K, Terao K, Toxic effect of HS-6 toxin produced by Nocardia strain. Agric Biol Chem 1990; 54: 2737–2739.Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Kiyoshi Terao
    • 1
  • Emiko Ito
    • 1
  • Motoko Oarada
    • 1
  • Misako Ohkusu
    • 1
  • Katsukiyo Yazawa
    • 1
  • Yuzuru Mikami
    • 1
  • Kazuei Igarashi
    • 2
  1. 1.Research Center for Pathogenic Fungi and Microbial ToxicosesChiba UniversityChibaJapan
  2. 2.Faculty of Pharmaceutical ScienceChiba UniversityChibaJapan

Personalised recommendations