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Auxotrophic markers and the cytogenetics of Filobasidiella neoformans

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In the sexual phase of Cryptococcus neoformans four post-meiotic nuclei give rise to the nuclei of four long chains of basidiospores. If there is a fixed relationship between one post-meiotic nucleus and one chain the result should be genetic homogeneity within single chains; random composition of chains would represent the opposite case. We tested for homogeneity in single chains from crosses of niacin-auxotrophs to the wild type by dissecting single chains of basidiospores from mature fruiting bodies. Out of 14 chains, 11 were homogeneous; the probability of obtaining this result by random processes was <0.01. These results suggest that mitotic products of a particular post-meiotic nucleus may be channeled into a particular basidiospore chain.

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Kline, M.T., Jacobson, E.S. Auxotrophic markers and the cytogenetics of Filobasidiella neoformans. Mycopathologia 76, 115–117 (1981). https://doi.org/10.1007/BF00443758

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  • Random Process
  • Fruiting Body
  • Opposite Case
  • Single Chain
  • Cryptococcus Neoformans