Antonie van Leeuwenhoek

, Volume 45, Issue 3, pp 499–511 | Cite as

[1-14C] Acetate assimilation by obligate methylotrophs, Pseudomonas methanica and Methylosinus trichosporium

  • R. N. Patel
  • S. Louise Hoare
  • D. S. Hoare


The oxidation of one carbon compounds (methane, methanol, formaldehyde, formate) and primary alcohols (ethanol, propanol, butanol) supported the assimilation of [1-14C]acetate by cell suspensions of type I obligate methylotroph; Pseudomonas methanica, Texas strain, and type II obligate methylotroph, Methylosinus trichosporium, strain PG. The amount of oxygen consumed and substrate oxidized correlated with the amount of [1-14C]acetate assimilated during oxidation of C-1 compounds and primary alcohols.

Oxidation of methanol, formaldehyde, and primary alcohols in extracts of Pseudomonas methanica, Texas strain, and Methylosinus trichosporium, strain PG, was catalyzed by a phenazine methosulfate linked, ammonium ion dependent methanol dehydrogenase. The oxidation of aldehydes was catalyzed by a phenazine methosulfate linked, ammonium ion independent aldehyde dehydrogenase. Formate was oxidized by a NAD+ linked formate dehydrogenase.


Methanol Formaldehyde Aldehyde Butanol Propanol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© H. Veenman & Zonen B. V. 1979

Authors and Affiliations

  • R. N. Patel
    • 1
  • S. Louise Hoare
    • 1
  • D. S. Hoare
    • 1
  1. 1.Department of MicrobiologyUniversity of TexasAustinUSA

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