Cellular glycogen, β-1,2-glucan, poly-β-hydroxybutyric acid and extracellular polysaccharides in fast-growing species of Rhizobium
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Synthesis of acidic exopolysaccharides, neutral cellular polysaccharides and poly-β-hydroxybutyric acid (PHB) by Rhizobium is strongly dependent on cultural conditions and the strains used. Exopolysaccharide production by R. leguminosarum, R. Phaseoli and R. trifolii closely parallels growth, whereas R. meliloti mainly excretes (low mol wt) polysaccharides when cell propagation is limited by lack of a necessary growth element (nitrogen) and an excess of carbon source is still present in the medium.
In all strains, accumulation of cellular glycogen, β-1,2-glucan and PHB is initiated only under growth-limiting conditions. When the external carbon source is exhausted, glycogen and PHB are metabolized by the cells, sustaining their longevity and thus act as true reserve materials; on the other hand, β-1,2-glucan and excreted polysaccharides are not utilized on further incubation of the culture.
Differences exist in the nature and relative amounts of the products synthesized by strains of different species of Rhizobium. R. leguminosarum, R. phaseoli and R. trifolii synthesize a uronic acid-containing exopolysaccharide, PHB and/or glycogen, non-metabolizable capsular polysaccharide and low amounts of β-1,2-glucan. R. meliloti synthesizes a uronic acid-free exopolysaccharide, PHB and/or glycogen and high concentrations of β-1,2-glucan.
Exopolysaccharides, β-1,2-glucan and glycogen preparations were obtained by isolation and purification from cells of fast-growing species of Rhizobium and chemically characterized.
KeywordsPolysaccharide Carbon Source Cultural Condition Glucan Cell Propagation
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- Albersheim, P., Nevin, D. J., English, P. D. and Karr, A. 1967. A method for the analysis of sugars in plant cell-wall polysaccharides by gas-liquid chromatography. — Carbohydr. Res. 5: 340–345.Google Scholar
- Blumenkrantz, M. and Asboe-Hansen, G. 1973. New method for quantitative determination of uronic acids. — Anal. Biochem. 54: 484–489.Google Scholar
- Burton, R. M. 1957. The determination of glycerol and dihydroxyacetone. p. 246–249. In S. P. Colowick and N. O. Kaplan (eds), Methods in enzymology, Part III. — Academic Press, New York.Google Scholar
- Craig, A. S. and Williamson, K. I. 1972. Three inclusions of rhizobial bacteroids and their cytochemical character. — Arch Microbiol. 87: 165–171.Google Scholar
- Deinema, M. H. and Zevenhuizen, L. P. T. M. 1971. Formation of cellulose fibrils by Gram-negative bacteria and their role in bacterial flocculation. — Arch. Microbiol. 78: 42–57.Google Scholar
- Dudman, W. F. 1977. The role of surface polysaccharides in natural environments. p. 357–414. In I. W. Sutherland, (ed.), Surface carbohydrates of the procariotic cell. — Academic Press, London and New York.Google Scholar
- Ghai, S. K., Hisamatsu, M., Amemura, A. and Harada, T. 1981. Production and chemical composition of extracellular polysaccharides of Rhizobium. — J. Gen. Microbiol. 122: 33–40.Google Scholar
- Gorin, P. A. J. and Mazurek, M. 1973. Carbon-13 resonance spectroscopic studies on the formation of borate and diphenylborinate complexes of polyhydroxy compounds. — Can. J. Chem. 51: 3277–3286.Google Scholar
- Gorin, P. A. J., Spencer, J. F. T. and Westlake, D. W. S. 1961. The structure and resistance of methylation of 1,2-β-glucans from species of Agrobacterium. — Can. J. Chem. 39: 1067–1073.Google Scholar
- Harada, T. and Amemura, A. 1981. Bacterial β-glycans: succinoglycan and curdlan. — Mem. Inst. Sci. Ind. Res. Osaka University 38: 37–49.Google Scholar
- Hisamatsu, M., Abe, J., Amemura, A. and Harada, T. 1980. Structural elucidation on succinoglycan and related polysaccharides from Agrobacterium and Rhizobium by fragmentation with two special β-d-glycanases and methylation analysis. — Agri. Biol. Chem. 44: 1049–1055.Google Scholar
- Jansson, P. E., Lindberg, B. and Ljunggren, H. 1979. Structural studies of the Rhizobium trifolii extracellular polysaccharide. — Carbohydr. Res. 75: 207–220.Google Scholar
- Katsuki, H., Yoshida, T., Tanegashima, C. and Tanaka, S. 1971. Improved direct method for determination of keto acids by 2,4-dinitrophenylhydrazine. — Anal. Biochem. 43: 349–356.Google Scholar
- Krisman, C. R. 1962. A method for the colorimetric estimation of glycogen with iodine. — Anal. Biochem. 4: 17–23.Google Scholar
- McIntire, F. C. Peterson, W. H. and Riker, A. J. 1942. A polysaccharide produced by the crown-gall organism. — J. Biol. Chem. 143: 491–496.Google Scholar
- Patel, J. J. and Gerson, T. 1974. Formation and utilization of carbon reserves by Rhizobium. —Arch. Microbiol. 101: 211–220.Google Scholar
- Robertson, B. K., Aman, P., Darvill, A. G., McNeil, M. and Albersheim, P. 1981. Host-symbiont interactions V. The structure of acidic extracellular polysaccharides secreted by Rhizobium leguminosarum and Rhizobium trifolii. — Plant Physiol. 67: 389–400.Google Scholar
- Trevelyan, W. E. and Harrison, J. S. 1952. Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. — Biochem. J. 50: 298–310.Google Scholar
- Tsien, H. C. and Schmidt, E. L. 1981. Localization and partial characterization of soybean lectin-binding polysaccharide of Rhizobium japonicum. — J. Bacteriol. 145: 1063–1074.Google Scholar
- York, W. S., McNeil, M., Darvill, A. G. and Albersheim, P., 1980. Host-symbiont interactions. VIII. β-2-Linked glucans secreted by fast-growing species of Rhizobium. — J. Bacteriol. 142: 243–248.Google Scholar
- Zevenhuizen, L. P. T. M. 1971. Chemical composition of exopolysaccharides of Rhizobium and Agrobacterium. — J. Gen. Microbiol. 68: 239–243.Google Scholar
- Zevenhuizen, L. P. T. M. 1973. Methylation analysis of acidic exopolysaccharides of Rhizobium and Agrobacterium. — Carbohydr. Res. 26: 409–419.Google Scholar
- Zevenhuizen, L. P. T. M. and Ebbink, A. G. 1974. Interrelations between glycogen, poly-β-hydroxybutyric acid and lipids during accumulation and subsequent utilization in a Pseudomonas. — Antonie van Leeuwenhoek 40: 103–120.Google Scholar
- Zevenhuizen, L. P. T. M. and Scholten-Koerselman, H. J. 1979. Surface carbohydrates of Rhizobium. I. β-1,2-Glucans. — Antonie van Leeuwonhoek 45: 165–175.Google Scholar