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Antonie van Leeuwenhoek

, Volume 47, Issue 6, pp 481–497 | Cite as

Cellular glycogen, β-1,2-glucan, poly-β-hydroxybutyric acid and extracellular polysaccharides in fast-growing species of Rhizobium

  • L. P. T. M. Zevenhuizen
Physiology and Growth

Abstract

Synthesis of acidic exopolysaccharides, neutral cellular polysaccharides and poly-β-hydroxybutyric acid (PHB) by Rhizobium is strongly dependent on cultural conditions and the strains used. Exopolysaccharide production by R. leguminosarum, R. Phaseoli and R. trifolii closely parallels growth, whereas R. meliloti mainly excretes (low mol wt) polysaccharides when cell propagation is limited by lack of a necessary growth element (nitrogen) and an excess of carbon source is still present in the medium.

In all strains, accumulation of cellular glycogen, β-1,2-glucan and PHB is initiated only under growth-limiting conditions. When the external carbon source is exhausted, glycogen and PHB are metabolized by the cells, sustaining their longevity and thus act as true reserve materials; on the other hand, β-1,2-glucan and excreted polysaccharides are not utilized on further incubation of the culture.

Differences exist in the nature and relative amounts of the products synthesized by strains of different species of Rhizobium. R. leguminosarum, R. phaseoli and R. trifolii synthesize a uronic acid-containing exopolysaccharide, PHB and/or glycogen, non-metabolizable capsular polysaccharide and low amounts of β-1,2-glucan. R. meliloti synthesizes a uronic acid-free exopolysaccharide, PHB and/or glycogen and high concentrations of β-1,2-glucan.

Exopolysaccharides, β-1,2-glucan and glycogen preparations were obtained by isolation and purification from cells of fast-growing species of Rhizobium and chemically characterized.

Keywords

Polysaccharide Carbon Source Cultural Condition Glucan Cell Propagation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Albersheim, P., Nevin, D. J., English, P. D. and Karr, A. 1967. A method for the analysis of sugars in plant cell-wall polysaccharides by gas-liquid chromatography. — Carbohydr. Res. 5: 340–345.Google Scholar
  2. Blumenkrantz, M. and Asboe-Hansen, G. 1973. New method for quantitative determination of uronic acids. — Anal. Biochem. 54: 484–489.Google Scholar
  3. Burton, R. M. 1957. The determination of glycerol and dihydroxyacetone. p. 246–249. In S. P. Colowick and N. O. Kaplan (eds), Methods in enzymology, Part III. — Academic Press, New York.Google Scholar
  4. Craig, A. S. and Williamson, K. I. 1972. Three inclusions of rhizobial bacteroids and their cytochemical character. — Arch Microbiol. 87: 165–171.Google Scholar
  5. Deinema, M. H. and Zevenhuizen, L. P. T. M. 1971. Formation of cellulose fibrils by Gram-negative bacteria and their role in bacterial flocculation. — Arch. Microbiol. 78: 42–57.Google Scholar
  6. Dudman, W. F. 1977. The role of surface polysaccharides in natural environments. p. 357–414. In I. W. Sutherland, (ed.), Surface carbohydrates of the procariotic cell. — Academic Press, London and New York.Google Scholar
  7. Ghai, S. K., Hisamatsu, M., Amemura, A. and Harada, T. 1981. Production and chemical composition of extracellular polysaccharides of Rhizobium. — J. Gen. Microbiol. 122: 33–40.Google Scholar
  8. Gorin, P. A. J. and Mazurek, M. 1973. Carbon-13 resonance spectroscopic studies on the formation of borate and diphenylborinate complexes of polyhydroxy compounds. — Can. J. Chem. 51: 3277–3286.Google Scholar
  9. Gorin, P. A. J., Spencer, J. F. T. and Westlake, D. W. S. 1961. The structure and resistance of methylation of 1,2-β-glucans from species of Agrobacterium. — Can. J. Chem. 39: 1067–1073.Google Scholar
  10. Harada, T. and Amemura, A. 1981. Bacterial β-glycans: succinoglycan and curdlan. — Mem. Inst. Sci. Ind. Res. Osaka University 38: 37–49.Google Scholar
  11. Hisamatsu, M., Abe, J., Amemura, A. and Harada, T. 1980. Structural elucidation on succinoglycan and related polysaccharides from Agrobacterium and Rhizobium by fragmentation with two special β-d-glycanases and methylation analysis. — Agri. Biol. Chem. 44: 1049–1055.Google Scholar
  12. Jansson, P. E., Lindberg, B. and Ljunggren, H. 1979. Structural studies of the Rhizobium trifolii extracellular polysaccharide. — Carbohydr. Res. 75: 207–220.Google Scholar
  13. Katsuki, H., Yoshida, T., Tanegashima, C. and Tanaka, S. 1971. Improved direct method for determination of keto acids by 2,4-dinitrophenylhydrazine. — Anal. Biochem. 43: 349–356.Google Scholar
  14. Krisman, C. R. 1962. A method for the colorimetric estimation of glycogen with iodine. — Anal. Biochem. 4: 17–23.Google Scholar
  15. McIntire, F. C. Peterson, W. H. and Riker, A. J. 1942. A polysaccharide produced by the crown-gall organism. — J. Biol. Chem. 143: 491–496.Google Scholar
  16. Patel, J. J. and Gerson, T. 1974. Formation and utilization of carbon reserves by Rhizobium. —Arch. Microbiol. 101: 211–220.Google Scholar
  17. Robertson, B. K., Aman, P., Darvill, A. G., McNeil, M. and Albersheim, P. 1981. Host-symbiont interactions V. The structure of acidic extracellular polysaccharides secreted by Rhizobium leguminosarum and Rhizobium trifolii. — Plant Physiol. 67: 389–400.Google Scholar
  18. Trevelyan, W. E. and Harrison, J. S. 1952. Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. — Biochem. J. 50: 298–310.Google Scholar
  19. Tsien, H. C. and Schmidt, E. L. 1981. Localization and partial characterization of soybean lectin-binding polysaccharide of Rhizobium japonicum. — J. Bacteriol. 145: 1063–1074.Google Scholar
  20. York, W. S., McNeil, M., Darvill, A. G. and Albersheim, P., 1980. Host-symbiont interactions. VIII. β-2-Linked glucans secreted by fast-growing species of Rhizobium. — J. Bacteriol. 142: 243–248.Google Scholar
  21. Zevenhuizen, L. P. T. M. 1971. Chemical composition of exopolysaccharides of Rhizobium and Agrobacterium. — J. Gen. Microbiol. 68: 239–243.Google Scholar
  22. Zevenhuizen, L. P. T. M. 1973. Methylation analysis of acidic exopolysaccharides of Rhizobium and Agrobacterium. — Carbohydr. Res. 26: 409–419.Google Scholar
  23. Zevenhuizen, L. P. T. M. and Ebbink, A. G. 1974. Interrelations between glycogen, poly-β-hydroxybutyric acid and lipids during accumulation and subsequent utilization in a Pseudomonas. — Antonie van Leeuwenhoek 40: 103–120.Google Scholar
  24. Zevenhuizen, L. P. T. M. and Scholten-Koerselman, H. J. 1979. Surface carbohydrates of Rhizobium. I. β-1,2-Glucans. — Antonie van Leeuwonhoek 45: 165–175.Google Scholar

Copyright information

© Kluwer Academic Publishers 1981

Authors and Affiliations

  • L. P. T. M. Zevenhuizen
    • 1
  1. 1.Laboratory of MicrobiologyAgricultural UniversityWageningenThe Netherlands

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