, Volume 78, Issue 2, pp 107–122 | Cite as

Morphogenesis throughout saprobic and parasitic cycles of Coccidioides immitis

  • M. Huppert
  • S. H. Sun
  • J. L. Harrison


The fungus, Coccidioides immitis, differs from other dimorphic pathogens in that its parasitic stage is a complex morphogenic cycle, raising the question that changes in structure and composition during morphogenesis might influence host responses. As a prelude to examining the interaction of fungal morphogenesis and host responses, the life cycle of this fungus has been examined in greater detail than previously accomplished. During saprobic development, alternating enterothallic arthroconidia are formed as infectious propagules. The outer wall is broken and loosely adherent. Under in vitro conditions supporting the parasitic cycle, multinucleate arthroconidia transform into uninucleate round cells. Rapid, synchronous, nuclear replication is initiated, accompanied by increase in cell mass and deposition of new cell wall substance. As karyokinesis ceases, morphologic differentiation begins with invagination of the inner layers of the spherule wall and then is progressive, eventually segmenting the protoplasm into uninucleate endospores grouped in clusters within a hyaline membrane. Endospores, escaping through a break in the spherule wall, are held in aggregates by fibrils which are stretched and broken as endospores separate. It would seem that rapid production of hundreds of progeny from an original single cell, protected during development by an enclosing spherule wall and then released in clusters, should favor establishment of the fungus in a host, and dynamic changes in the cell wall during morphogenesis should influence the host response.


Cell Wall Fibril Cell Mass Dynamic Change Host Response 
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  1. 1.
    Baker, O. & Braude, A. I., 1956. A study of stimuli leading to the production of spherules in coccidioidomycosis. J. Lab. Clin. Med. 47: 169–181.Google Scholar
  2. 2.
    Bartels, P. A., Wagoner, N. & Larsh, H. W., 1968. Conversion of Coccidioides immitis in tissue culture. Mycopathol. Mycol. Applic. 35: 37–41.Google Scholar
  3. 3.
    Breslau, A. M., Hensley, T. J. & Erickson, J. O., 1961. Electron microscopy of cultured spherules of Coccidioides immitis. J. Biophys. Biochem. Cytol. 9: 627–637.Google Scholar
  4. 4.
    Brueck, J. W. & Buddingh, G. J., 1951. Propagation of pathogenic fungi in the yolk sac of embryonated eggs. Proc. Soc. Exper. Biol. Med. 76: 332–335.Google Scholar
  5. 5.
    Cole, G. T. & Samson, R. A., 1979. Patterns of development in conidial fungi. Fearon Pitman Publ. Inc., Belmont, Calif.Google Scholar
  6. 6.
    Collins, M. S. & Pappagianis, D., 1973. Effects of lysozyme and chitinase on the spherules of Coccidioides immitis in vitro. Infect. Immun. 7: 817–822.Google Scholar
  7. 7.
    Collins, M. S., Pappagianis, D. & Yee, J., 1977. Enzymatic solubilization of precipitin and complement fixing antigen from endospores, spherules, and spherule fraction of Coccidioides immitis, p. 429–444. In: L. Ajello (ed.), Coccidioidomycosis: Current clinical and diagnostic status. Symposia Specialists, Miami, Fla.Google Scholar
  8. 8.
    Donnelly, W. H. & Yunis, E. J., 1974. The ultrastructure of Coccidioides immitis. Study of a human infection. Arch. Pathol. 94: 227–232.Google Scholar
  9. 9.
    Drutz, D. J. & Catanzaro, A., 1978. Coccidioidomycosis. Am. Rev. Resp. Dis. 117: 559–585, 727–771.Google Scholar
  10. 10.
    Emmons, C. W., 1967. Fungi which resemble Coccidioides immitis, p. 333–337. In: L. Ajello (ed.), Coccidioidomycosis: Proceedings second coccidioidomycosis symposium. Univ. Ariz. Press, Tucson, Ariz.Google Scholar
  11. 11.
    Fiese, M. J., 1958. Coccidioidomycosis. Charles C. Thomas, Publ., Springfield, Ill.Google Scholar
  12. 12.
    Forbes, W. D. & Bestebreurtje, A. M., 1946. Coccidioidomycosis, a study of 95 cases of the disseminated type with special reference to the pathogenesis of the disease. Milit. Surg. 59: 653–719.Google Scholar
  13. 13.
    Huntington, R. W., 1957. Diagnostic and biologic implications of the histopathology of coccidioidomycosis, p. 36–46. In: Proceedings of symposium on coccidioidomycosis. U. S. Publ. Health. Serv. Public. No. 575.Google Scholar
  14. 14.
    Huntington, R. W., 1980. Pathology of coccidioidomycosis, p. 113–132. In: D. A. Stevens (ed.), Coccidioidomycosis: A text. Plenum Med. Book Co., New York, N.Y.Google Scholar
  15. 15.
    Huppert, M., Adler, J. P., Rice, E. H. & Sun, S. H., 1979. Common antigens among systemic disease fungi analyzed by two-dimensional immunoelectrophoresis. Infect. Immun. 23: 479–485.Google Scholar
  16. 16.
    Huppert, M., Kinsey, N., Sun, S. H., Vukovich, K. R. & Rice, E. H., 1978. Antigenic analysis of coccidioidin and spherulin determined by two-dimensional immunoelectrophoresis. Infect. Immun. 20: 541–551.Google Scholar
  17. 17.
    Huppert, M., Sun, S. H. & Bailey, J. W., 1967. Natural variability in Coccidioides immitis, p. 323–328. In: L. Ajello (ed.), Coccidioidomycosis: Proceedings second coccidioidomycosis symposium. Univ. Ariz. Press, Tucson, Ariz.Google Scholar
  18. 18.
    Kendrick, B. (ed.), 1971. Taxonomy of fungi imperfecti. Univ. Toronto Press, Toronto, Queb.Google Scholar
  19. 19.
    Kong, Y.-C. M., Levine, H. B. & Smith, C. E., 1963. Immunogenic properties of nondisrupted and disrupted spherules of Coccidioides immitis in mice. Sabouraudia 2: 131–142.Google Scholar
  20. 20.
    Kwon-Chung, K. J., 1969. Coccidioides immitis: Cytological study on the formation of arthrospores. Canad. J. Genet. Cytol. 11: 43–53.Google Scholar
  21. 21.
    Levine, H. B., Cobb, J. M. & Smith, C. E., 1960. Immunity to coccidioidomycosis induced in mice by purified spherule, arthrospore, and mycelial vaccines. Trans. N. Y. Acad. Sci. 22: 436–449.Google Scholar
  22. 22.
    Levine, H. B., Pappagianis, D. & Cobb, J. M., 1970. Development of vaccines for coccidioidomycosis. Mycopathol. Mycol. Applic. 41: 177–185.Google Scholar
  23. 23.
    Maddy, K. T., 1965. Observations on Coccidioides immitis found growing naturally in soil. Ariz. Med. 22: 281–288.Google Scholar
  24. 24.
    Moore, M., 1941. The chorioallantoic membrane of the developing chick as a medium for the cultivation and histopathologic study of pathogenic fungi. Am. J. Pathol. 17: 103–120.Google Scholar
  25. 25.
    O'Hern, E. M. & Henry, B. S., 1956. A cytological study of Coccidioides immitis by electron microscopy. J. Bacteriol. 72: 632–645.Google Scholar
  26. 26.
    O'Hern, E. M. & Henry, B. S., 1957. An electron microscope study of Coccidioides immitis, p. 191–197. In: Proceedings of symposium on coccidioidomycosis. U. S. Publ. Health Serv. Public. No. 575.Google Scholar
  27. 27.
    Savage, D. C. & Madin, S. H., 1968. Cellular responses in lungs of immunized mice to intranasal infection with Coccidioides immitis. Sabouraudia 6: 94–102.Google Scholar
  28. 28.
    Sigler, L. & Carmichael, J. W., 1976. Taxonomy of Malbranchea and some other hyphomycetes with arthroconidia. Mycotaxon 4: 349–488.Google Scholar
  29. 29.
    Smith, C. E., Saito, M. T., Beard, R. R., Kepp, R. M., Clark, R. W. & Eddie, B. U., 1950. Serological tests in the diagnosis and prognosis of coccidioidomycosis. Am. J. Hyg. 52: 1–21.Google Scholar
  30. 30.
    Smith, C. E., Saito, M. T. & Simons, S. A., 1956. Pattern of 39,500 serologic tests in coccidioidomycosis. J. Am. Med. Assoc. 160: 546–552.Google Scholar
  31. 31.
    Smith, C. E., Whiting, E. G., Baker, E. E., Rosenberger, H. G., Beard, R. R. & Saito, M. T., 1948. The use of coccidioidin. Am. Rev. Tuberc. 57: 330–360.Google Scholar
  32. 32.
    Smith, H., 1977. Microbial surfaces in relation to pathogenicity. Bacteriol. Rev. 41: 475–500.Google Scholar
  33. 33.
    Sun, S. H. & Huppert, M., 1976. A cytological study of morphogenesis in Coccidioides immitis. Sabouraudia 14: 185–198.Google Scholar
  34. 34.
    Sun, S. H., Sekhon, S. S. & Huppert, M., 1979. Electron microscopic studies of saprobic and parasitic forms of Coccidioides immitis. Sabouraudia 17: 265–273.Google Scholar
  35. 35.
    Tarbet, J. E. & Breslau, A. M., 1953. Histochemical investigation of the spherule of Coccidioides immitis in relation to host reaction. J. Infect. Dis. 92: 183–190.Google Scholar

Copyright information

© Dr W. Junk Publishers 1982

Authors and Affiliations

  • M. Huppert
    • 1
  • S. H. Sun
    • 1
  • J. L. Harrison
    • 1
  1. 1.Audie L. Murphy Memorial Veterans Hospital and University of Texas Health Science CenterSan AntonioUSA

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