Advertisement

European Journal of Pediatrics

, Volume 137, Issue 1, pp 5–10 | Cite as

Aldolase activities of the small intestinal mucosa in malabsorption states and hereditary fructose intolerance

  • H. Streb
  • H. G. Posselt
  • K. Wolter
  • S. W. Bender
Original Investigations

Abstract

Determination of aldolase activity in intestinal biopsy material offers a diagnostic alternative to liver biopsy in hereditary fructose intolerance (HFI). This diagnostic method could be validated by analysis of intestinal biopsies from eight patients with HFI. With the substrate fructose-1-phosphate (fru-1-p) we found 0.3±0.3 (x±SD) U/g of protein, with the substrate fructose-1,6-diphosphate (fru-1,6-p) 3.8±2.7 U/g of protein were measured. These results differ clearly from control activities (substrate = fru-1-p: 7.4±1.9 U/g of protein; substrate=fru-1,6-p: 13.9±4.3 U/g of protein). As the performance of the intestinal biopsy—in contrast to the liver biopsy—is virtually free of complications, it is recommended as the diagnostic method of choice in this disease.

Aldolase activities were also determined in 40 intestinal biopsies from children with different malabsorption states: celiac disease (n=13), cow's milk protein intolerance (n=9), postinfectious syndrome (n=10), giardiasis (n=8). The activities differed clearly from our results in hereditary fructose intolerance. In celiac disease, cow's milk protein intolerance and postinfectious syndrome characteristic activity patterns were obtained: activities with fru-1,6-p were increased (celiac disease 17.2±5.7 U/g of protein; cow's milk protein intolerance 19.5±7.0 U/g of protein) or in the normal range (postinfectious syndrome 14.0±3.1 U/g or protein); activities with fru-1-p were decreased (celiac disease 2.3±1.0 U/g of protein; cow's milk protein intolerance 3.4±1.3 U/g of protein; postinfectious syndrome 5.0±0.8 U/g of protein). These results are discussed on the basis of aldolase isoenzyme distribution in the small intestinal mucosa.

Key words

Hereditary fructose intolerance Aldolase activity Small intestine Malabsorption states 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bergmeyer HU, Bernt E (1974) Fructose-1,6-diphosphat-Aldolase. UV-Test, manuelle Methode. In: Bergmeyer, Hill (Hrsg) Methoden der enzymatischen Analyse. Verlag Chemie, Weinheim, pp 1142–1147Google Scholar
  2. 2.
    Chiprut RO, Greenwald RA, Morris SJ, Zeppa R, Schiff ER (1978) Intrahepatic hematoma resulting in obstructive jaundice. Gastroenterology 74:124–127Google Scholar
  3. 3.
    Creamer B, Croft DN (1970) Losses from the gut in the coeliac syndrome. In: Booth CC, Dowling RH (eds) Coeliac disease. Churchill-Livingstone, Edinburgh London, pp 21–25Google Scholar
  4. 4.
    Dahlquist A (1964) Method of assay of intestinal disaccharidases. Anal Biochem 7:18Google Scholar
  5. 5.
    Hamilton JR, Gall DG, Butler DG, Middleton PJ (1976) Viral gastroenteritis: recent progress, remaining problems. In: Acute diarrhoea in childhood, Ciba foundation symposium 42. Elsevier Excerpta Medica, North Holland, pp 209–222Google Scholar
  6. 6.
    Lebherz HG, Rutter WJ (1969) Distribution of fructose diphosphate aldolase variants in biological systems. Biochemistry 8:109–121Google Scholar
  7. 7.
    Leuthardt F, Wolf HP (1974) 1-Phosphofructaldolase. In: Bergmeyer, Hill (Hrsg) Methoden der enzymatischen Analyse. Verlag Chemie, Weinheim, pp 1151–1154Google Scholar
  8. 8.
    Levin B, Snodgrass GHAI, Oberholzer VG, Burgess EA, Dobbs RH (1968) Fructosaemia. Amer J Med 45:826–838Google Scholar
  9. 9.
    Loehry CA, Creamer B (1969) Three-dimensional structure of the rat small intestinal mucosa related to mucosal dynamics. Gut 10:112–120Google Scholar
  10. 10.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  11. 11.
    MacDonald TT, Ferguson A (1978) Small intestinal epithelial cell kinetics and protozoal infection in mice. Gastroenterology 74:496–500Google Scholar
  12. 12.
    Metais P, Juif J, Sacrez R (1962) Etude biochimique d'un cas d'intolérance au fructose. Ann Biol Clin 20:801–811Google Scholar
  13. 13.
    Nisell J, Linden L (1968) Fructose-1-phosphate aldolase and fructose-1,6-diphosphate aldolase activity in the mucosa of the intestine in hereditary fructose intolerance. Scand J Gastroenterol 3:80–82Google Scholar
  14. 14.
    Penhoet E, Kochman M, Rutter WJ (1969) Molecular and catalytic properties of aldolase C. Biochemistry 8:4396–4402Google Scholar
  15. 15.
    Rehbein-Thöner M, Pfleiderer G (1977) The changes in aldolase isoenzyme pattern during development of the human kidney and small intestine—demonstrated in organ extract and tissue sections. Hoppe-Seyler's Z Physiol Chem 358:169–180Google Scholar
  16. 16.
    Rosensweig NS, Stifel FB, Herman RH, Zakim D (1968) The dietary regulation of the glycolytic enzymes. II. Adaptive changes in human jejunum. Biochim Biophys Acta 170:228–234Google Scholar
  17. 17.
    Sachs L (1978) Der Vergleich zweier unabhängiger Stichproben: U-Test von Wilcoxon, Mann und Whitney. In: Angewandte Statistik, Springer-Verlag, pp 230–238Google Scholar
  18. 18.
    Schaub J, Osang M (1976) Angeborene Enzymdefekte des Kohlenhydratstoffwechsels. Münch Med Wochenschr 118: 609–614Google Scholar
  19. 19.
    Shmerling DH (1970) Peroral intestinal mucosal biopsies in infants and children. Helv paediat Acta Suppl 22:1–20Google Scholar
  20. 20.
    Silber D, Checinska E, Rabczynski J, Kochman M (1975) Isozyme pattern of fructose diphosphate aldolase during hepatocarcinogenesis induced by 2-acetylaminofluorenen in rat liver. Int J Cancer:675–681Google Scholar
  21. 21.
    Snodgrass DR, Ferguson A, Allan F, Angus KW, Mitchell B (1979) Small intestinal morphology and epithelial cell kinetics in lamb rotavirus infections. Gastroenterology 76: 477–481Google Scholar
  22. 22.
    Steinmann B, Gitzelmann R (1981) The diagnosis of hereditary fructose intolerance. Helv paediat Acta, submittedGoogle Scholar
  23. 23.
    Townley RRW, Barnes GL (1973) Intestinal biopsy in childhood. Arch Dis Childh 48:480–482Google Scholar
  24. 24.
    Trier JS, Browining TH (1970) Epithelial cell renewal in cultured duodenal biopsies in celiac sprue. New Engl J Med 283:1245–1250Google Scholar
  25. 25.
    Wachsmuth ED (1976) Differentiation of epithelial cells in human jejunum: localization and quantification of aminopeptidase, alkaline phosphatase and aldolase isozymes in tissue sections. Histochemistry 48:101–109Google Scholar
  26. 26.
    Wachsmuth ED, Stoye JP (1976) A classification of tumor development based on an analysis of enzymes in tissue sections of hypernephroid carcinoma in man. Beitr Path 159:229–248Google Scholar
  27. 27.
    Wright N, Watson A, Morley A, Appleton D, Marks J (1973) Cell kinetics in flat (avillous) mucosa of the human small intestine. Gut 14:701–710Google Scholar
  28. 28.
    Yardley JH, Takano J, Hendrix TR (1964) Epithelial and other mucosal lesions of the jejunum in giardiasis. Jejunal biopsy studies. Bull Hopkins Hosp 115:389–406Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • H. Streb
    • 1
  • H. G. Posselt
    • 1
  • K. Wolter
    • 1
  • S. W. Bender
    • 1
  1. 1.Abteilung Allgemeine Pädiatrie I, Zentrum der KinderheilkundeKlinikum der Johann-Wolfgang-Goethe-Universität FrankfurtFederal Republic of Germany

Personalised recommendations