Advertisement

Mycopathologia

, Volume 136, Issue 3, pp 119–123 | Cite as

Phospholipase activity in Cryptococcus neoformans

  • V. VidottoEmail author
  • A. Sinicco
  • D. Di Fraia
  • S. Cardaropoli
  • S. Aoki
  • S. Ito-Kuwa
Human and Animal Mycology

Abstract

Phospholipases have only been detected in a few fungi and yeasts, in particular in Candida albicans. Secreted phospholipases are considered by some researchers to be a potential factor of virulence and pathogenicity in C. albicans. Twenty-three Cryptococcus neoformans strains were tested in order to observe phospholipase production. Twenty-two of the 23 strains tested were able to produce phospholipases, and the ratio diameter of the colony to total diameter of the colony plus zone of precipitation (Pz) ranged between 0.271 and 0.949. C. neoformans, just like C. albicans, can be divided on the basis of the Pz into different strains according to their virulence and pathogenicity. There also appeared to be a correlation between the phospholipase production and the size of the capsule in the strains isolated from AIDS patients. For this reason, further studies on C. neoformans phospholipase activity would be useful in evaluating the virulence of different strains.

Key words

Cryptococcus neoformans phospolipase virulence AIDS 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Hanel H, Kirsch R, Schmidts H, Kottmann H. New systematically active antimycotics from the beta-blocker category. Mycoses 1995; 38: 251–264.CrossRefPubMedGoogle Scholar
  2. 2.
    Banno Y, Yamada T, Nozawa Y. Secreted phospholipases of the dimorphic fungus Candida albicans; separation of three enzymes and some biological properties. J Med Vet Mycol 1985; 23: 47–54.CrossRefGoogle Scholar
  3. 3.
    Goyal S, Khuller GK. Phospholipids and subcellular distribution in yeast and mycelial form of Candida albicans. J Med Vet Mycol 1992; 30: 355–362.CrossRefPubMedGoogle Scholar
  4. 4.
    Ibrahim AS, Mirbord F, Filler SG, Banno Y, Cole GT, Kitajiama Y, Edwards Jr JE, Nozawa Y, Ghammoum MA. Evidence implicating phospholipase as a virulence factor of Candida albicans. Infect and Immun 1995; 63: 1993–1998.Google Scholar
  5. 5.
    Kyung SL, Patton JL, Fido M, Hines LK, Kohlwein SD, Paltauf F, Henry SA, Levin GE. The Saccharomyces cerevisiae PLB1, gene encodes a protein required for lysphospholipase and phospholipase B activity. J Biol Chem 1994; 269: 1975–1980.Google Scholar
  6. 6.
    Odds FC. Pathogenesis of Candidosis. In: Odds FC. Candida and Candidosis 2nd ed. London: Baillière-Tindall 1988: 252–278.Google Scholar
  7. 7.
    Price MF, Wilkinson ID, Gentry LO. Plate method for detection of phospholipase activity in Candida albicans. Sabouraudia 1982; 20: 7–14.PubMedGoogle Scholar
  8. 8.
    Pugh D, Cawson RA. The cytochemical localization of phospholipase in Candida albicans infecting the chick chonriallantoic membrane. Sabouraudia 1977; 15: 23–35.Google Scholar
  9. 9.
    Samaranayake LP, Raeside JM, MacFarlane TW. Factors affecting the phospholipase activity of Candida species in vitro. Sabouraudia: J Med Vet Mycol 1984; 22: 201–207.CrossRefGoogle Scholar
  10. 10.
    Currie BP, Casadevall A. Estimation of the prevalence of cryptococcal infection among patients infected with the human immunodeficiency virus in New York city. Clin Infect Dis 1994; 19: 1029–1033.PubMedGoogle Scholar
  11. 11.
    Cross CE, Bancroft GJ. Ingestion of acapsular Cryptococcus neoformans via mannose and beta-glucan receptors, resulting in cytokine production and increased phagocytosis of the encapsulated form. Infect Immun 1995; 63: 2604–2611.PubMedGoogle Scholar
  12. 12.
    Durden FM, Elewski B. Cutaneous involvement with Cryptococcus neoformans in AIDS. J Acad Dermatol 1994; 30: 844–848.CrossRefGoogle Scholar
  13. 13.
    Perduca N, Marangoni E, Guanziroli A, Romero E, Filice G. Fungaemia in hospitalized patients. Mycoses 1995; 38: 385–387.CrossRefPubMedGoogle Scholar
  14. 14.
    Sugar AM. Overview: cryptococcosis in the patients with AIDS. Mycopathologia 1991; 114: 153–157.CrossRefPubMedGoogle Scholar
  15. 15.
    Yamamoto Y, Kohno S, Noda T, Kakeya H, Yanagihara K, Ohno H, Ogawa K, Kawamura S, Ohtsubo T, Tomono K et al. Isolation of Cryptococcus neoformans from environments (pigeon excreta) in Nagasaki. Kansenshogaku Zasshi 1995; 69: 642–645.PubMedGoogle Scholar
  16. 16.
    Ghannoum MA, Abu-Elteen K. Correlative relationship between proteinase production, adherence and pathogenicity of various strains of Candida albicans. J Med Vet Mycol 1986; 24: 407–441.CrossRefPubMedGoogle Scholar
  17. 17.
    Polak A. Virulence of Candida albicans mutants. Mycoses 1992; 35: 9–16.CrossRefPubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • V. Vidotto
    • 1
    Email author
  • A. Sinicco
    • 1
  • D. Di Fraia
    • 1
  • S. Cardaropoli
    • 1
  • S. Aoki
    • 2
  • S. Ito-Kuwa
    • 2
  1. 1.Laboratorio Micologia MedicaDipartimento discipline Medico-Chirurgiche Università di Torino, Sez. Malattie InfettiveTorinoItaly
  2. 2.General Research InstituteNippon Dental UniversityNiigataJapan

Personalised recommendations