Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Studies on detection of Candida antigen in the sera of mice inoculated orally with Candida albicans

  • 22 Accesses

  • 9 Citations

Summary

The authors succeeded in establishing a murine model of systemic candidiasis being disseminated from the primary gastrointestinal lesions caused by oral inoculation of Candida albicans. Using this model, an attempt was made for detecting the Candida antigen by enzyme-linked immunosorbent assay using avidin-biotin (AB-ELISA) from the serum of infected mice.

Gastrointestinal candidiasis was formed in all of the 20 mice treated with the drugs (antibiotics, antineoplastic agents, hydrocortisone, etc.) and inoculated orally with C. albicans. Fourteen of these mice suffered from submucosal candidiasis, and C. albicans was cultured from the visceral organs in 12 of them. The assay by AB-ELISA was able to detect 1.0 ng/ml Candida mannan in the mouse serum. The Candida antigen was detected in the sera of 11 of the 14 mice with submucosal candidiasis. However, the antigen could not be detected in the sera of the 6 mice with intramucosal candidiasis.

The assay by AB-ELISA is more sensitive and specific for the diagnosis of systemic candidiasis than other serological assays.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Armstrong D, Young LS, Meyer RD, Blevis AH. Infectious complications of neoplastic disease. Med Clin North Am 1971; 55:729–745.

  2. 2.

    Baley JW, Sada E, Brass C, Bennet JE. Diagnosis of systemic candidiasis by Latex agglutination for serum antigen. J Clin Microbiol 1985; 21:749–752.

  3. 3.

    Baley JE, Kliegman RM, Fonaroff AA. Disseminated fungal infections in very low birth-weight infants: Clinical manifestation and epidemiology. Pediatrics 1984; 73:144–152.

  4. 4.

    Bodey GP. Fungal infections complicating acute leukemia. J Chronic Dis 1966; 19:667–687.

  5. 5.

    DeMaria A, Buckley H, von Lichtenberg F. Gastrointestinal candidiasis in rats treated with antibiotics, cortisone, and azathioprine. Infect Immun 1976; 13:1761–1770.

  6. 6.

    de Repentigny L, Kuykendall RJ, Chandler FW, Broderson JR, Reiss E. Comparison of serum mannan, arabinitol, and mannose in experimental disseminated candidiasis. J Clin Microbiol 1984; 19:804–812.

  7. 7.

    Eras P, Goldstein MJ, Sherlock P. Candida infection of the gastrointestinal tract. Medicine 1972; 51:675–681.

  8. 8.

    Faix RG. Systemic Candida infections in infants in intensive care nurseries: High incidence of central nervous involvement. J Pediatr 1984; 105:616–622.

  9. 9.

    Fillice GB Yu, Armstrong D. Immunodiffusion and agglutination tests for Candida in patients with neoplastic disease. J Infect Dis 1977; 135:349–357.

  10. 10.

    Helstrom PB, Balish E. Effect of oral tetracycline, the microbial flora, and the athymic state on gastrointestinal colonization and infections of BALB/c mice with Candida albicans. Infect Immun 1979; 23:764–774.

  11. 11.

    Holak E, Wu J, Spruance SL. Value of serum arabinitol for the management of Candida infection in clinical practice. Mycopathologia 1986; 93:99–104.

  12. 12.

    Hotchi M, Okada M, Nasu T. Present state of fungal infections in autopsy cases in Japan: A statistical survey of all autopsy cases during the ten-year period from 1966 to 1975. Am J Clin Pathol 1980; 74:410–416.

  13. 13.

    Hughes JM, Smith DR. Infection during induction of remission in acute lymphocytic leukemia. Cancer 1973; 31:1108–1114.

  14. 14.

    Hummel RP, Oestreicher MP, Maley MP, MacMillan BG. Inhibition of Candida albicans by Escherichia coli in in vitro and in the germ-free mouse. J Surg Res 1973; 15:53–58.

  15. 15.

    Huppert M, Cazin J, Smith H. Pathogenesis of Candida albicans infection following antibiotics therapy. 3. The effect of antibiotics on the incidence of Candida albicans in the intestinal tracts of mice. J Bacteriol 1955; 70:440–447.

  16. 16.

    Johnson DE, Thompson TR, Green TP, Ferrier P. Systemic candidiasis in very low birth-weight infants (<1500 grams) Pediatric 1984; 137:197–201.

  17. 17.

    Kennedy MJ, Volz PA. Dissemination of yeasts after gastrointestinal inoculation in antibiotics-treated mice Sabouraudia 1983; 21:27–33.

  18. 18.

    Kennedy MJ, Volz PA. Effect of various antibiotics on gastrointestinal colonization and dissemination by Candida albicans. Sabouradia 1985; 23:265–273.

  19. 19.

    Kerkering TM, Espinel-Ingroff A, Shadny S. Detection of Candida antigenemia by counterimmunoelectrophoresis in patients with invasive candidiasis. J Infect Dis 1979; 140:659–664.

  20. 20.

    Kiehn TE, Bernard EM, Gold JWM, Fong D, Silber A, Armstrong D. Candidiasis: Detection by gas-liquid chromatography of D-arabinitol, a fungal metabolite, in human serum. Science 1979; 206:577–580.

  21. 21.

    Krause W, Matheis H, Wulf K. Fungemia and funguria after oral administration of Candida albicans. Lancet 1969; i:598–599.

  22. 22.

    Lew MA, Donachue DM, Mariorca F. Enhanced detection with an enzyme-linked immunosorbent assay of Candida mannan in antibody-coated serum after heat extraction. J Infect Dis 1982; 145:45–56.

  23. 23.

    Lowry O, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193:265–270.

  24. 24.

    Marier R, Andriole VT. Usefulness of serial antibody determination in diagnosis of candidiasis as measured by discontinuous counterimmunoelectrophoresis using HS antigen. J Clin Microbiol 1978; 8:15–22.

  25. 25.

    Miller CG, Witwer MW, Braude AI, Davis CE. Rapid identification of Candida albicans septicemia in man by gasliquid chromatography. J Clin Invest 1974; 54:2135–2240.

  26. 26.

    Myerowitz RL, Pazin GL, Allen C. Disseminated candidiasis: Changes in incidence, underlying disease, and pathology. Am J Clin Pathol 1977; 68:29–38.

  27. 27.

    Nerurkar LS, Jacob AJ, Madden DL, Sever JL. Detection of genital herpes simplex infections by tissue culture fluorescent-antibody technique with biotin-avidin. J Clin Microbiol 1983; 17:149–154.

  28. 28.

    Nishikawa T, Hatano H, Ohnishi N, Sasaki S, Nomura T. Establishment of Candida albicans in the alimentary tract of the germ-free mice and antagonism with Escherichia coli after oral inoculation. Jpn J Microbiol 1969; 13:263–276.

  29. 29.

    Peat S, Whelan WJ, Edward TE. Polysaccharides of baker's yeast. J Chem Soc (London) 1961; 1:29–34.

  30. 30.

    Pope LM, Cole GT, Guentzel MN, Berry LJ. Systemic and gastrointestinal candidiasis of infant mice after intragastric challenge. Infect Immun 1979; 25:702–707.

  31. 31.

    Reiss E, Stockman L, Kuykendall R, Smith SJ. Dissociation of mannan-serum complexes and detection of Candida albicans mannan by enzyme immunosorbent assay variations. Clin Chem 1982; 28:306–310.

  32. 32.

    Scheld WM, Brown RS Jr, Harding SA, Samde MA. Detection of circulating antigen in experimental Candida albicans endocarditis by enzyme-linked immunosorbent assay. J Clin Microbiol 1980; 12:679–683.

  33. 33.

    Segal E, Berg RA, Pizzo PA, Bennet JE. Detection of circulating antigen in sera of patients with candidiasis by an enzyme-linked immunosorbent assay inhibition technique. J Clin Microbiol 1980; 10:116–118.

  34. 34.

    Slavin RE, Dias MA, Saral R. Cytosine arabinoside induced gastrointestinal toxic alternation in sequential chemotherapeutic protocols: A clinical-pathologic study of 33 patients. Cancer 1978; 42:1747–1759.

  35. 35.

    Suzuki H, Nakamura A, Taguchi H, Nishimura K, Miyaji M. Detection of serum Candida antigen by an enzymelinked immunosorbent assay with avidin-biotin system: 1 Methods and basic study. Kansensyougaku Zasshi (in Japanese) 1986; 60:336–344.

  36. 36.

    Voller A, Bidwell D, Bartlett A. Enzyme-linked immunosorbent assay. In: Rose NR, Friedmann H, eds. Manual of clinical immunology, 2nd ed. Washington DC: American Society for Microbiology, 1980: 359–379.

  37. 37.

    Warren RC, Bartlett A, Bidwell DE, Richardson MD, Voller A, White LO. Diagnosis of invasive candidiasis by enzyme immunoassay of serum antigen. Br Med J 1977; 1:1183–1185.

  38. 38.

    Weiner MH, Yount WJ. Mannan antigenemia in the diagnosis of invasive Candida infection. J Clin Invest 1976; 58:1045–1053.

  39. 39.

    Weiner MH, Coats-Stephen M. Immunodiagnosis of systemic candidiasis: Mannan antigenemia detected by radioimmunoassay in experimental and human infection. J Infect Dis 1979; 140:989–993.

  40. 40.

    Wingard JR, Dick JD, Merz WG, Sandford GR, Saral R, Burns WH. Pathogenesity of Candida tropicalis and Candida albicans after gastrointestinal inoculation in mice. Infect Immun 1980; 29:808–813.

Download references

Author information

Correspondence to Hiroshi Suzuki.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Suzuki, H., Taguchi, H., Nishimura, K. et al. Studies on detection of Candida antigen in the sera of mice inoculated orally with Candida albicans . Mycopathologia 104, 7–17 (1988). https://doi.org/10.1007/BF00437918

Download citation

Key words

  • systemic candidiasis
  • gastrointestinal candidiasis
  • Candida mannan antigen
  • enzyme-linked immunosorbent assay using avidin-biotin (AB-ELISA)