Molecular Breeding

, Volume 2, Issue 4, pp 369–379 | Cite as

Identification of peanut (Arachis hypogaea L.) RAPD markers diagnostic of root-knot nematode (Meloidogyne arenaria (Neal) Chitwood) resistance

  • Mark D. Burow
  • Charles E. Simpson
  • Andrew H. Paterson
  • James L. Starr
Research Paper

Abstract

DNA markers linked to a root-knot nematode resistance gene derived from wild peanut species have been identified. The wild diploid peanut accessions K9484 (Arachis batizocoi Krapov. & W. C. Gregory), GKP10017, (A. cardenasii Krapov & W. C. Gregory), and GKP10602 (A. diogoi Hoehne) possess genes for ressitance to Meloidogyne arenaria. These three accessions and A. hypogaea cv. Florunner were crossed to generate the hybrid resistant breeding line TxAg-7. This line was used as donor parent to develop a BC4F2 population segregating for resistance. Three RAPD markers associated with nematode resistance were identified in this population by bulked segregant analysis. Linkage was confirmed by screening 21 segregatingh BC4F2 and 63 BC5F2 single plants. Recombination between marker RKN410 and resistance, and between marker RKN440 and resistance, was estimated to be 5.4±1.9% and 5.8±2.1%, respectively, on a per-generation basis. These two markers identified a resistance gene derived from either A. cardenasii or A. diogoi, and were closely linked to each other. Recombination between a third marker, RKN229, inherited from A. cardenasii or A. diogoi, and resistance was 9.0±3.2% per generation. Markers RKN410 and RKN229 appeared to be linked genetically and flank the same resistance gene. All markers were confirmed by hybridization of cloned or gel-purified marker DNA to blots of PCR-amplified DNA. Pooled data on the segregation of BC5F2 plants was consistent with the presence of one resistance gene in the advanced breeding lines. Different distributions of resistance in the BC5F2 progeny and TxAG-7 suggest the presence of additional resistance genes in TxAG-7.

Key words

genetic mapping DNA marker-assisted selection introgression bulked segregant analysis 

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References

  1. 1.
    Burow MD, Reddy MCM, Starr JL, Simpson CE, Paterson AH: A new method for the extraction of DNA from peanut (Arachis spp.) (in preparation).Google Scholar
  2. 2.
    Cap GB, Roberts PA, Thomason IJ: Inheritance of heat-stable resistance to Meloidogyne incognita in Lycopersicon peruvianum and its relationship to the Mi gene. Theor Appl Genet 85: 777–783 (1993).Google Scholar
  3. 3.
    Chittenden LM, Schertz KF, Lin Y-R, Wing RA, Paterson AH: A detailed RFLP map of Sorghum bicolor × S. propinquum, suitable for high-density mapping suggests ancestral duplication of Sorghum chromosomes or chromosomal segments. Theor Appl Genet 87: 925–933 (1994).Google Scholar
  4. 4.
    Conner BJ, Reyes A, Morin C, Itakura K, Teplitz RL, Wallace RB. Detection of sickle cell β s-globin allele by hybridization with synthetic oligonucleotides. Proc Natl Acad Sci USA 80: 278–282 (1983).Google Scholar
  5. 5.
    Fry WE: Principles of Plant Disease Management. Academic Press, New York (1982).Google Scholar
  6. 6.
    Giovannoni JJ, Wing RA, Ganal MW, Tanksley SD: Isolation of molecular markers from specific chromosomal intervals using DNA pools from existing mapping populations. Nucl Acids Res 19: 6553–6558 (1991).Google Scholar
  7. 7.
    Haldane JBS Waddington CH: Inbreeding and linkage. Genetics 16: 358–374 (1931).Google Scholar
  8. 8.
    Halward T, Stalker T, LaRue E, Kochert G: Genetic variation detectable with molecular markers among unadapted germplasm resources of cultivated peanut and related wild species. Genome 34: 1013–1020 (1991).Google Scholar
  9. 9.
    Harris MK, Frederiksen RA: Concepts and methods regarding host plant resistance to arthropods and pathogens. Ann Rev Phytopath 22: 247–272 (1984).Google Scholar
  10. 10.
    Hartl L, Weiss H, Stephen U, Zeller FJ, Jahoor A: Molecular identification of powdery mildew resistance genes in common wheat (Triticum aestivum L.) Theor Appl Genet 90: 601–606 (1995)Google Scholar
  11. 11.
    Holbrook CC, Noe JP: Resistance to peanut root-knot nematode (Meloidogyne arenaria) in Arachis hypogaea. Peanut Sci 19: 35–37 (1992).Google Scholar
  12. 12.
    Hussey RS, Barker KR: A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Dis Rep 57: 1025–1028 (1973).Google Scholar
  13. 13.
    Ingram EG, Rodríguez-Kabana R: Nematodes parasitic on peanut in Alabama and evaluation of methods for extraction and study of population dynamics. Nematropica 10: 21–30 (1980).Google Scholar
  14. 14.
    Johnson J: A critical analysis of durable resistance. Annu Rev Phytopath 22: 309–330 (1984).Google Scholar
  15. 15.
    Johnson E, Miklas PN, Stavely JR, Martinez-Cruzado JC: Coupling- and repulsion-phase RAPDs for marker-assisted selection of PI 181996 rust resistance in common bean. Theor Appl Genet 90: 659–664 (1995).Google Scholar
  16. 16.
    Kosambi DD. The estimation of map distances from recombination values. Ann Eugenics 12: 172–175 (1944).Google Scholar
  17. 17.
    Krapovickas A, Gregory WC: Taxonomía del género Arachis (Leguminosae). Bonplandia 8: 1–186 (1994).Google Scholar
  18. 18.
    Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L: MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174–181 (1987).Google Scholar
  19. 19.
    Liu S-C, Kowalski P, Lan T-H, Feldmann KA, Paterson AH: Genome-wide high-resolution mapping by recurrent intermating, using Arabidopsis thaliana as a model. Genetics 142: 247–258 (1996).Google Scholar
  20. 20.
    Luzzi BM, Boerma HR, Hussey RS: Inheritance of resistance to the peanut root-knot nematode in soybean. Crop Sci 35: 50–53 (1995).Google Scholar
  21. 21.
    Martin GB, Brommonschenkel SH, Chunwongse J, Frary A, Ganal MW, Spiven R, Tanksley SD: Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262: 1432–1436 (1993).Google Scholar
  22. 22.
    Mather K: The Measurement of Linkage in Heredity, 2nd ed. John Wiley, New York (1951).Google Scholar
  23. 23.
    MathSoft, Inc.: MathCAD. Cambridge, MA (1988).Google Scholar
  24. 24.
    Messeguer R, Ganal M, DeVicente MC, Young ND, Bolkan H, Tanksley SD: High resolution RFLP map around the root-knot nematode resistance gene (Mi) in tomato. Theor Appl Genet 82: 529–536 (1991).Google Scholar
  25. 25.
    Michelmore RW, Paran I, Kesseli RV: Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregating populations. Proc Natl Acad Sci USA 88: 9828–9832 (1991).Google Scholar
  26. 26.
    Motsinger R, Crawford J, Thompson S: Nematode survey of peanuts and cotton in southwest Georgia. Peanut Sci 3: 72–74 (1976).Google Scholar
  27. 27.
    Muehlbauer GJ, Staswick PE, Specht JE, Graef GL, Shoemaker RD, Keim P: RFLP mapping using near-isogenic lines in the soybean [Glycine max (L.) Merr.] Theor Appl Genet 81: 189–198 (1991).Google Scholar
  28. 28.
    Nelson SC, Simpson CE, Starr JL: Resistance to Meloidogyne arenaria in Arachis spp germplasm. Suppl J Nematol 21: S654-S660 (1989).Google Scholar
  29. 29.
    Nelson SC, Starr JL, Simpson CE: Expression of resistance to Meloidogyne arenaria in Arachis batizocoiand A.cardenasii. J Nematol 22: 423–425 (1990).Google Scholar
  30. 30.
    Paterson AH, Lin Y-R, Li Z, Schertz KF, Doebley JF, Pinson SRM, Liu S-C, Stansel JW, Irvine JE: Convergent domestication of cereal crops by independent mutations at corresponding loci. Science 269: 1714–1718 (1995).Google Scholar
  31. 31.
    Porter DM, Smith DH, Rodriguez-Kabana R: Peanut plant disease. In Pattee HE, Young CT (eds) Peanut Science and Technology, pp, 326–410. American Peanut Research and Education Society, Yoakum, TX (1982).Google Scholar
  32. 32.
    Rodríguez-Kabana R, King PS: Evaluation of selected nematicides for control of Meloidogyne arenaria in peanut: a multiple year study. Nematropica 15: 155–164 (1985).Google Scholar
  33. 33.
    Sambrook J., Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Press, Cold Spring Harbor, NY (1989).Google Scholar
  34. 34.
    SAS Institute. SAS. Cary, NC (1989).Google Scholar
  35. 35.
    Sasser JN, Freckman DW: A world perspective on nematology. In Veech JA, Dickson DE (eds) Vistas on Nematology, pp. 7–14. Society of Nematologists, Hyattsville, MD (1987).Google Scholar
  36. 36.
    Simpson CE, Nelson SC, Starr JL, Woodward KE, Smith OD: Registration of TxAG-6 and TxAG-7 peanut germplasm lines. Crop Sci 33: 1418 (1993).Google Scholar
  37. 37.
    Snedecor GW, Cochran WG: Statistical Methods, 7th ed., pp. 287–288. Iowa State University Press, Ames, IO (1980).Google Scholar
  38. 38.
    Stalker HT, Moss JP: Speciation, cytogenetics, and utilization of Arachis species. Adv Agron 41: 1–40 (1987).Google Scholar
  39. 39.
    Starr JL, Schuster GL, Simpson CE: Characterization of the resistance to Meloidogyne arenaria in an interspecific Arachis spp. hybrid. Peanut Sci 17: 106–108 (1990).Google Scholar
  40. 40.
    Starr JL, Simpson CE: Segregation of resistance of Meloidogyne arenaria in progeny of interspecific hybrids. Proc Am Peanut Res Educ Soc 23: 23 (1991).Google Scholar
  41. 41.
    Starr JL, Simpson CE, Lee TA Jr: Resistance to Meloidogyne arenaria in advanced generation breeding lines of peanut. Peanut Sci 22: 59–61 (1995).Google Scholar
  42. 42.
    Wang GL, Paterson AH: Assessment of DNA pooling strategies for mapping of QTLs. Theor Appl Genet 88: 355–361 (1994).Google Scholar
  43. 43.
    Wheeler TA, Starr JL: Incidence and economic importance of plant-parasitic nematodes on peanut in Texas. Peanut Sci 14: 94–96 (1987).Google Scholar
  44. 44.
    Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18: 6531–6535 (1991).Google Scholar
  45. 45.
    Williamson VM, Ho JY, Wu FF, Miller N, Kaloshian I: A PCR-based marker tightly-linked to the nematode resistance gene, Mi in tomato. Theor Appl Genet 87: 757–763 (1994).Google Scholar
  46. 46.
    Wynne JC, Halward T: Cytogenetics and genetics of Arachis. Crit Rev Plant Sci 8: 189–220 (1989).Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Mark D. Burow
    • 1
  • Charles E. Simpson
    • 2
  • Andrew H. Paterson
    • 1
  • James L. Starr
    • 3
  1. 1.Department of Soil and Crop ScienceTexas A&M UniversityCollege StationUSA
  2. 2.Texas Agricultural Experiment StationTexas A&M UniversityStephenvilleUSA
  3. 3.Department of Plant Pathology and MicrobiologyTexas A&M UniversityCollege StationUSA

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