Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Effect of antineoplastic agents and X-irradiation on the adherence of Candida spp. to human buccal epithelial cells in vitro

  • 8 Accesses

  • 6 Citations


The role of chemotherapy, X-irradiation and a combination of both on the phenomenon of adherence of yeast to buccal epithelial cells (BEC) was investigated in vitro. Growth of three Candida spp. in the presence of eight of eleven antineoplastic agents led to reduction of adherence of the isolates tested (reduction between 30% and 61% of the control value), and this effect was observed whether exponential or stationary phase Candida cells were used. Exposure of C. albicans to various doses of radiation also led to a reduction in adherence of this yeast to BEC between 31% and 53% of the control value. This reduction was shown to be dose related. Similar results were obtained when BEC were exposed to radiation, and the effect of radiation treatment was accentuated when both yeast and BEC were irradiated simultaneously. Furthermore, treating C albicans with a combination of chemotherapy and radiation led to the greatest reduction in adherence of yeast to BEC compared to when the yeast was treated with either chemotherapy or radiation alone (reduction between 63% to 74% as compared with control). The possible mechanism/s involved in reduction of adherence of yeast to BEC are discussed.

This is a preview of subscription content, log in to check access.


  1. 1.

    Albert A. The covalent bond in selective toxicity. In: Selective toxicity: the physico-chemical basis of therapy. London: Chapman and Hall, 1973:392–423.

  2. 2.

    Alkan ML, Beachy E. Excretion of lipoteichoic acid by group A Streptococci: Influence of penicillin on excretion and loss of ability to adhere to human oral epithelial cells. J Clin Inves 1978; 61:671–7.

  3. 3.

    Bases R. Enhancement of X-ray damage in Hela cells by exposure to lucanthone (Miracyl D) following radiation. Cancer Res 1970; 30:2007–11.

  4. 4.

    Campbell AC, Hersey P, Maclennan ICM, Kay HEM, Pike MC and the Medical Research Council's working party on Leukaemia in childhood. Immunosuppresssive consequences of radiotherapy and chemotherapy in patients with Acute Lymphoblastic Leukaemia. Brit Med J 1973; 2:385–8.

  5. 5.

    Cho SY, Choy HY. Opportunistic fungal infection among cancer patients. Am J Clin Pathol 1979; 72:617–21.

  6. 6.

    Dermoumi H. Differentiation of yeast-like fungi isolated from clinical specimen. Aerztt Lab 1979; 25:289–91.

  7. 7.

    Douglas LJ. Adhesion to surfaces. In: Rose AH, Harrison JS, eds. The yeasts, vol 2. London: Academic Press, 1987:239–80.

  8. 8.

    Elkind MM, Whitmore GF, Alescio T. Actinomycin D: suppression of recovery in X-irradiated mammalian cells. Science 1964; 143:1454–6.

  9. 9.

    Ghannoum MA, Al-Khars A. Effect of antineoplastic agents on the growth and ultrastructure of Candida albicans. Mykosen 1984; 27:452–64.

  10. 10.

    Ghannoum MA, Khattar MS, Kayed F, Shridar P. The influence of antineoplastic agents on experimental candidosis in mice. Mykosen 1985; 28:369–87.

  11. 11.

    Ghannoum MA, Motawy MS, Mubarak AL, Al-Awadhi HA. Incidence and susceptibility of yeasts isolated from cancer patients in Kuwait. Mykosen 1985; 28:619–24.

  12. 12.

    Ghannoum MA, Motawy MS, Mubarak AL, Al-Awadhi HA. Candida albicans strain differentiation in cancer patients undergoing therapy. Mykosen 1985; 28:388–93.

  13. 13.

    Gibbons RJ, Adhesion of bacteria to surfaces of the mouth. In: Berkely RCW, Lynch JM, Melling JM, Rutter PR, Vincent B, eds. Microbial adhesion to surfaces. Chichester: Horwood, 1980.

  14. 14.

    Gordee RS, Simpson PJ. Relationships of X-irradiation to the enhancement of Candida albicans infections. J Bacteriol 1967; 94:6–12.

  15. 15.

    Henderson SD, Kimler BF. Response to cultured 9L cells to bleomycin and X-rays. Abstract and Twenty-eight annual meeting of the Radiation Research Society, New Orleans, Louisiana. Radiation Research 1980; 83:372.

  16. 16.

    Hersh EM, Freireich EJ. Host defense mechanisms and their modifications by cancer chemotherapy. In: Busch H, ed. Methods in cancer, Vol 4. New York: New York Research Academic Press, 1968:355.

  17. 17.

    Kiehn TE, Edwards FF, Armstrong D. The prevalence of yeasts in clinical specimens from cancer patients. Am J Clin Pathol 1980; 73:518–21.

  18. 18.

    King RD, Lee JC, Morris AL. Adherence of Candida albicans and other Candida species to mucosal epithelial cells. Infect Immun 1980; 27:667–74.

  19. 19.

    Kimler BF. Interaction of radiation and DHAQ: effect on cell survival. Abstract at twenty-eighth annual meeting of the Radiation Research Society, New Orleans, Louisiana. Radiation Res 1980; 83:372.

  20. 20.

    Kimura L, Pearsall N. Adherence of Candida albicans to human buccal epithelial cells. Infect Immun 1978; 21:64–8.

  21. 21.

    Kondo S. Radiation genetics in microorganisms and evolutionary considerations. Genetics 1974; 78:149–61.

  22. 22.

    Lee JC, King RD. Characterization of Candida albicans adherence to human vaginal epithelial cells in vitro. Infect Immun 1983; 41:1024–30.

  23. 23.

    Lindberg RD. Present day role of radiation therapy in the treatment of head and neck cancer. In: Chambers RG, Janssen De Limpens AMP, Jaques DA, Routledge RT, eds. Cancer of the Head and Neck, Proceedings of an International Symposium, Montreux, Switzerland. New York: American Elsevier, 1975:7–13.

  24. 24.

    Maisch PA, Calderone RA. Role of surface mannan in the adherence of Candida albicans to fibrin-platelet clots formed in vitro. Infect Immun 1981; 32:92–7.

  25. 25.

    Moore CW. Responses of radiation-sensitive mutants of Saccharomyces cerevisiae to lethal effects of bleomycin. Mutation Res 1978; 51:165–80.

  26. 26.

    Newman K, Schimpff SC, Wade JC. Antibiotic prophylaxis of infection for patients with granulocytopenia. In: Verhoef J, Peterson PK, Quie PG, eds. Infection in the immunocompromised host-pathogenesis, prevention and therapy. Amsterdam: Elsevier/North-Holland Biomedical Press, 1980:187–206.

  27. 27.

    Paul J. Media for culturing cells and tissues. II Defined media. In: Cell and tissue culture. Edinburgh: Churchill Livingstone, 1975:90–123.

  28. 28.

    Radman M. SOS repair hypothesis: Phenomenology of an inducible DNA repair which is accompanied by mutagenesis. In: Hanawalt P, Setlow RB, eds. Molecular mechanisms for repair of DNA. New York: Plenum Press, 1975:355–67.

  29. 29.

    Richards GJ, Chambers RG. Combined therapy in the treatment of carcinomas of the head and neck. In: Chambers RG, Janssen De Limpens AMP, Jaques DA, Routledge RT, eds. Cancer of the head and neck. Proceedings of an International Symposium, Montreux, Switzerland. New York: American Elsevier, 1975:23–32.

  30. 30.

    Samaranayake L, Macfarlane T. Factors affecting the in vitro adherence of the fungal oral pathogen Candida albicans to epithelial cells of human origin. Arch Oral Biol 1982; 27:869–73.

  31. 31.

    Segal E, Lehrer N, Ofek I. Adherence of Candida albicans to human vaginal epithelial cells: inhibition by amino sugars. Exp Cell Biol 1982; 50:13–7.

  32. 32.

    Shanta V, Krishnamurthi S. The combined therapy of oral cancer. In: Carter S, Ichikawa T, Mathe G, Umezawa H, eds. Fundamental and clinical studies of bleomycin, GANN Monograph on Cancer Research No 19. Baltimore: University Park Press, 1976:159–70.

  33. 33.

    Silverman S, Luangjarmekorn L, Greenspan D. Occurrence of oral Candida in irradiated head and neck cancer patients. J Oral Med 1984; 39:194–6.

  34. 34.

    Tattersall MH. Aggressive cancer treatment and its role in predisposing to infection. Eur J Cancer 1975; 11:9–19.

  35. 35.

    United Nations. Ionizing radiation: Sources and biological effects. In: United Nations Scientific Committee on the effects of atomic radiation. Report to the general assembly, with annexes. New York: United Nations, 1982:727–73.

  36. 36.

    Witkin EM. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev 1976; 40:869–907.

Download references

Author information

Correspondence to M. A. Ghannoum.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ghannoum, M.A., Abu-Elteen, K.H. & Motawy, M.S. Effect of antineoplastic agents and X-irradiation on the adherence of Candida spp. to human buccal epithelial cells in vitro . Mycopathologia 104, 171–180 (1988). https://doi.org/10.1007/BF00437433

Download citation

Key words

  • adherence
  • antineoplastic agents
  • Candida species
  • X-irradiation