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Dot-immunobinding assay in the detection of IgG antibodies against farmer's lung antigens

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Circulating antibodies against Faenia rectivirgula, Thermoactinomyces candidus, T. vulgaris and Aspergillus fumigatus were studied in the sera of 14 clinically proven farmer's lung patients and 10 normal controls using three immunological methods. These methods were agar gel double diffusion (DD), biotin-avidin-linked immunosorbent assay (BALISA) and dot-immunobinding assay (DIBA). Agar gel diffusion, the least sensitive of the three methods, failed to detect antibodies in some of the patients, while BALISA detected antibodies even in the normal controls. However, the sensitivity of dot-immunobinding assay was in between DD and BALISA while the specificity was comparable to DD to all the antibodies except against A. fumigatus antigens. Dot-immunobinding assay gave faster results than DD and the blots can be stored as record for longer periods of time without fading.

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  1. 1.

    Barboriak JJ, Fink JN, Scribner GH. Immunological crossreaction of thermophilic actinomycetes isolated from home environments. J Allergy Clin Immunol 1972; 49:81–85.

  2. 2.

    Bardana EJ. The clinical spectrum of aspergillosis. CRC Crit Rev Clin Lab Sci 1980; 13:21–83.

  3. 3.

    Fink JN. Hypersensitivity pneumonitis due to organic dusts. Clin Notes Respir Dis 1974; 13:3–9.

  4. 4.

    Flaherty DK, Barboriak JJ, Emanuel DA, Fink JN, Marx JJ, Moore VL, Reed CE, Roberts RC. Multi-laboratory comparison of three immunodiffusion methods used in the detection of precipitating antibodies in hypersensitivity pneumonitis. J Lab Clin Med 1974; 84:298–306.

  5. 5.

    Fuller CJ. Farmer's lung review of present knowledge. Thorax 1953; 8:59–64.

  6. 6.

    Kurup VP. Enzyme-linked immunosorbent assay in the detection of specific antibodies against Aspergillus in patient sera. Zbl Bakt Hyg [A] 1986; 261:509–516.

  7. 7.

    Kurup VP, Fink JN. A scheme for the identification of thermophilic actinomycetes associated with hypersensitivity pneumonitis. J Clin Microbiol 1975; 2:55–61.

  8. 8.

    Kurup VP, Fink JN. Extracellular antigens of Micropolyspora faeni grown in synthetic medium. Infect Immun 1977; 15:608–613.

  9. 9.

    Kurup VP, Barboriak JJ, Fink JN. Hypersensitivity Pneumonitis. In: Al-Doory Y, Domson JE, eds. Mould Allergy. Philadelphia: Lea and Febiger, 1984: 216–243.

  10. 10.

    Kurup VP, Fink JN, Scribner GH, Falk MJ. Antigenic variability of Aspergillus fumigatus strains. Microbios 1978; 19:191–204.

  11. 11.

    Kurup VP, Resnick A, Scribner GH, Gunasekaran M, Fink JN. The enzyme profile and immunochemical characterization of Aspergillus fumigatus antigens. J Allergy Clin Immunol 1986; 78:1166–1173.

  12. 12.

    Kurup VP, Mäntyjärvi RA, Therho EO, Ojanen TH, Kalbfleisch JH. Circulating IgG antibodies against fungal and actinomycete antigens in the sera of farmer's lung from different countries. Mycopathologia 1987: 98:91–99.

  13. 13.

    Lacey J. Actinomycetes in soils, composts and fodders. In: Sykes G, Skinner FA, eds. Actinomycetales. New York: Academic Press, 1973: 231–246.

  14. 14.

    Lacey J. The microbiology of hay and straw. In: de Haller R, Suter F, eds. Aspergillosis and farmer's lung in man and animal. Bern: Hans Huber Publishers, 1974: 16–23.

  15. 15.

    Pepys J. Hypersensitivity diseases of the lung due to fungi and other organic dusts. In: Kallos P, Jasek M, Inderbitzin TM, Miesches PA, Waksman BH, eds. Monographs in Allergy 4. Basel: S. Karger Co., 1969: 1–111.

  16. 16.

    Ramasamy M, Khan ZU, Kurup VP. A partially purified antigen from Faenia rectivirgula in the diagnosis of farmer's lung disease. Microbios 1987; 49:171–182.

  17. 17.

    Treuhaft MW, Roberts RC, Hackbarth C, Marx JJ. Characterization of synthetic medium antigens of Micropolyspora faeni and Thermoactinomyces Candidus. J Allergy Clin Immunol 1981; 67:375–387.

  18. 18.

    Wadsworth C. A microtemplate technique employing a gel chamber compared with other micro and macroplate techniques for immunodiffusion. Int Arch Allergy Appl Immunol 1962; 31:131–137.

  19. 19.

    Wenzel FJ, Grey RL, Roberts RC, Emanuel DA. Serologic studies in farmer's lung. Precipitins to thermophilic actinomycetes. Am Rev Respir Dis 1974; 109:464–468.

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Correspondence to Rauno M. Mäntyjärvi.

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Mäntyjärvi, R.M., Kurup, V.P. Dot-immunobinding assay in the detection of IgG antibodies against farmer's lung antigens. Mycopathologia 103, 49–54 (1988).

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Key words

  • Aspergillus
  • biotin-avidin linked immunosorbent assay
  • dot-immunobinding assay
  • double immunodiffusion
  • farmer's lung
  • thermophilic actinomycetes