, Volume 106, Issue 2, pp 109–112 | Cite as

Immunodiffusion test for diagnosing human pythiosis

  • P. Imwidthaya
  • S. Srimuang


An immunodiffusion test was developed for diagnosing subcutaneous and systemic pythiosis in humans. When culture filtrate antigen (CFA) from P. insidiosum was reacted against patient and rabbit antisera, 1–5 precipitin bands occured both in patient and rabbit antisera, and a line of identity also occured between patient and rabbit sera. When control P. insidiosum CFA was reacted with 30 apparently normal persons, 20 Thalassemia patients, 2 candidosis and 5 aspergillosis patients, no precipitin bands were found. P. insidiosum CFA also tested with rabbit antibodies to B. dermatitidis, C. immitis, H. capsulatum, P. brasiliensis, C. albicans, M. furfur and A. fumigatus revealed no cross reactions. This test is practical, sensitive and specific.

Key words

human pythiosis immunodiffusion 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Mendoza L, Alfaro AA. Equine pythiosis in Costa Rica: Report of 39 cases. Mycopathologia 1986; 94: 123–9.Google Scholar
  2. 2.
    Smith F. The pathology of bursattee. Vet J 1884; 19: 16–17.Google Scholar
  3. 3.
    Haan J, Hoogkamer LJ. Hyphomycosis destruens equine. Archiv Wiss Prakt Tierheilk 1903; 29: 395–410.Google Scholar
  4. 4.
    Bridges CH, Emmons CW. A phycomycosis of horse caused by Hyphomyces destruens. J Am Vet Med Assoc 1968; 138: 579–89.Google Scholar
  5. 5.
    Hutchins DR, Johnston KG. Phycomycosis in the horse. Aust Vet J 1972; 48: 269–78.Google Scholar
  6. 6.
    Austwick PKC, Copland JW. Swamp cancer. Nature 1974; 250: 84.Google Scholar
  7. 7.
    Chandler FW, Kaplan W, Ajello L. A color atlas and textbook of the histopathology of mycotic diseases. Chicago: Year Book Medical Publishers Inc., 1980; 104–5.Google Scholar
  8. 8.
    Mendoza L, Kaufman L, Standard PG. Immunodiffusion test for Diagnosis and Monitoring Pythiosis in Horses. J Clin Microbiol 1986; 23: 813–6.Google Scholar
  9. 9.
    Thianprasit M. Cutaneous Pythiosis in Human-Being. Abstract in Siriraj 100th Academic Meeting, Mahidol University, Bangkok, Thailand, 18–22 April 1988: 94.Google Scholar
  10. 10.
    De Cock AWAM, Mendoza L, Padhye AA, Ajello L, Kaufman L. Pythium insidiosum sp. nov., the etiologic agent of pythiosis. J Clin Microbiol 1987; 25: 344–9.Google Scholar
  11. 11.
    Sathapatayawongs B. Human Pythiosis. Siriraj 100th Academic Meeting, Mahidol University, Bangkok, Thailand, 18–22 April 1988: 258–62.Google Scholar
  12. 12.
    Miller RI, Campbell RSF. Immunological studies on equine phycomycosis. Aust Vet J 1982; 58: 227–31.Google Scholar

Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • P. Imwidthaya
    • 1
  • S. Srimuang
    • 2
  1. 1.Department of Microbiology, Siriraj HospitalMahidol UniversityBangkokThailand
  2. 2.Division of Infectious Disease and Host Defence Unit, Research Center, Faculty of Medicine, Ramathibodi HospitalMahidol UniversityBangkokThailand

Personalised recommendations