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Mycopathologia

, Volume 84, Issue 1, pp 41–44 | Cite as

Effect of yeast growth conditions on yeast-mycelial transition in Candida albicans

  • William M. Bell
  • W. LaJean Chaffin
Article

Abstract

When grown and induced to form germ tubes in liquid defined media, yeast cells of Candida albicans must reach stationary phase before acquiring ability to carry out the yeast-mycelial transition. This study examined the effect of the carbon source utilized for yeast growth on the inducibility of stationary phase yeast. When grown to the same stationary phase cell density as glucose cultures, cultures grown on citrate were fully inducible while cultures grown on galactose and mannose showed a small reduction. Cultures grown on ethanol were reduced 80% in morphological conversion. When glucose grown cells were induced in the presence of these carbon sources, hexoses supported full induction while ethanol reduced induction 80%. Induction in the presence of carboxylic acids was similar to induction in the absence of added carbon source. When induced on the same source used in yeast growth, germ tube formation was reduced for all carbon sources except hexoses. When induced in the absence of added carbon source, yeasts grown on citrate and ethanol were inhibited 80–100%. Cultures starved for glucose were more inhibited than cultures starved for NH4Cl when induced without added carbon source. These observations suggest that the metabolic state of the stationary phase cell is an important factor in the ability to respond to conditions inducing germ tube formation.

Keywords

Carbon Source Galactose Mannose Candida Albicans NH4Cl 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Dr W. Junk Publishers 1983

Authors and Affiliations

  • William M. Bell
    • 1
  • W. LaJean Chaffin
    • 1
  1. 1.Department of BiologyTexas Christian UniversityFt. WorthUSA

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