Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

TGA-Termination codon in the apocytochrome b gene from Oenothera mitochondria

  • 32 Accesses

  • 63 Citations

Summary

The apocytochrome b gene in the mitochondrial genome of Oenothera contains 1,185 base pairs (bp) in uninterrupted sequence. Comparison with the maize sequence indicates firstly that at the 5′ end of the gene an additional triplet, coding for alanine, is inserted immediately behind the initiator ATG. Secondly the carboxy-terminus of the polypeptide is extended by five amino acids. The only in-frame termination codon for the Oenothera gene is a TGA triplet 15 nucleotides downstream from the maize TAG termination, which corresponds to a GAG codon in Oenothera. The plant mitochondria) tryptophan codon CGG and TGG appear to be interchangable although a bias for TGG is observed in this gene (12:1). At position 718–720 in the Oenothera nucleotide sequence TGG is found where a CGG occurs in maize, and nucleotide 361 in Oenothera gene is a T, generating a TGG codon where in maize a CGG codon is found.

This is a preview of subscription content, log in to check access.

References

  1. Anderson S, Bankier AT, Barrell BC, De Brujin MHL, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreier HP, Smith AJH, Staden R, Young IG (1981) Nature 290:457–464

  2. Benne R, Agostinelli M, de Vries B, van den Burg J, Klaver B, Borst P (1983) In: Wolf K, Schweyen R, Kaudewitz F (eds) Mitochondria 1983. De Gruyter, Berlin New York, pp 285–302

  3. Bibb MJ, Van Etten RA, Wright CT, Walberg MW, Clayton DA (1981) Cell 26:167–180

  4. Brennicke A, Blanz P (1982) Mol Gen Genet 187:461–466

  5. Chao S, Sederoff RR, Levings CS III (1983) Plant Physiol 71:190–193

  6. Clary D, Goddard J, Martin S, Fauron C, Wolstenholme D (1982) Nucl Acids Res 10:6619–6637

  7. Coruzzi G, Bonitz SG, Thalenfeld BE, Tzagoloff A (1981) J Biol Chem 256:12780–12787

  8. Dawson AJ (1984) PhD Thesis, Edinburgh

  9. Dawson AJ, Jones VP, Leaver CJ (1984) EMBO J 3:2107–2113

  10. de Brujin M (1983) Nature 304:234–240

  11. Fox TD (1979) J Mol Biol 130:63–82

  12. Fox TD, Leaver CJ (1981) Cell 26:315–323

  13. Gray M, Spencer D (1983) FEBS Lett 161:323–327

  14. Hiesel R, Brennicke A (1983) EMBO J 2:2173–2178

  15. Leaver CJ, Hack E, Dawson AJ, Isaac PG, Jones VP (1983) In: Wolf K, Schweyen RJ, Kaudewitz F (eds) Mitochondria 1983. De Gruyter, Berlin New York, pp 269–283

  16. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning. Cold Spring Harbour Laboratory Press, NY

  17. Maxam AM, Gilbert W (1980) Methods Enzymol 65:499–560

  18. Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) J Mol Biol 113:237–245

  19. Saraste M (1984) FEBS Lett 166:367–372

  20. Seed B (1982) Nucl Acids Res 10:1799–1810

  21. Spencer DF, Schnare MN, Gray MW (1984) Proc Natl Acad Sci USA 81:493–497

  22. Taniguchi T, Weissmann C (1978) J Mol Biol 118:533–565

  23. Waring RB, Davies RW, Lee S, Grisi E, McPhail-Berks M, Scazzocchio C (1981) Cell 27:4–11

  24. Widger WR, Cramer WA, Herrmann R, Trebst A (1984) Proc Natl Acad Sci USA 81:674–678

Download references

Author information

Correspondence to Axel Brennicke.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Schuster, W., Brennicke, A. TGA-Termination codon in the apocytochrome b gene from Oenothera mitochondria. Curr Genet 9, 157–163 (1985). https://doi.org/10.1007/BF00436964

Download citation

Key words

  • Genetic code
  • Higher plant
  • mtDNA