, Volume 85, Issue 1–2, pp 75–79 | Cite as

Lack of carcinogenicity of agaritine by subcutaneous administration in mice

  • B. Toth
  • H. Sornson


Agaritine (A), an ingredient of the cultivated mushroom of commerce Agaricus bisporus, was administered by subcutaneous injection to two groups of randomly bred Swiss mice. In the first group the animals of both sexes were treated at a 100 μg/g body weight basis five times at weekly intervals, while in the second group the mice received a single A treatment of 100 μg/g body weight for females and 50 μg/g body weight for males. The administration of the compound resulted in no detectable carcinogenic effect in the animals. Since some of the breakdown products of A were shown to be carcinogenic in mice and the mushroom itself was found to be mutagenic, the field is discussed in the light of the obtained results.


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  1. 1.
    Daniels, E. G., Kelly, R. B. & Hinman, J. W., 1961. Agaritine: an improved isolation procedure and confirmation of structure by synthesis. J. Am. Chem. Soc. 83: 3333–3334.Google Scholar
  2. 2.
    Lawson, T. & Toth, B., 1983. Metabolic conversion of arylhydrazines of the cultivated mushroom Agaricus bisporus. Proc. Am. Assoc. Cancer Res. 24: 77.Google Scholar
  3. 3.
    Levenberg, B., 1961. Structure and enzymatic cleavage of agaritine, a phenylhydrazide of L-glutamic acid isolated from Agaricaceae. J. Am. Chem. Soc. 83: 503–504.Google Scholar
  4. 4.
    Rogan, E. G., Walker, B. A., Gingell, R., Nagel, D. & Toth, B., 1982. Microbial mutagenicity of selected hydrazines. Mut. Res. 102: 413–419.Google Scholar
  5. 5.
    Ross, A. E., Nagel, D. & Toth, B., 1982. Evidence for the occurrence and formation of diazonium ions in the Agaricus bisporus mushroom and its extracts. J. Agr. Food Chem. 30: 521–525.Google Scholar
  6. 6.
    Ross, A., Nagel, D. & Toth, B., 1982. Occurrence, stability and decomposition of β-N[γ-L(+)glutamyl]-4-hydroxyme-thylphenylhydrazine (agaritine) of the mushroom Agaricus bisporus. Food Chem. Toxicol. 20: 903–907.Google Scholar
  7. 7.
    Sterner, O., Bergman, R., Kesler, E., Magnusson, G., Wickberg, B. & Zimerson, E., 1982. Mutagens in larger fungi. I. Forty-eight species screened for mutagenic activity in the Salmonella/microsome assay. Mut. Res. 101: 269–281.Google Scholar
  8. 8.
    Toth, B., Rappaport, H. & Shubik, P., 1963. The influence of dose and age on the induction of malignant lymphomas and other tumors by 7,12-dimethylbenz(a)anthracene in Swiss mice. J. Natl. Cancer Inst. 30: 723–741.Google Scholar
  9. 9.
    Toth, B. & Wilson, R. B., 1971. Blood vessel tumorigenesis by 1,2-dimethylhydrazine dihydrochloride (symmetrical). Gross, light and electron microscopic descriptions. I. Am. J. Pathol. 64: 585–600.Google Scholar
  10. 10.
    Toth, B., 1972. A toxicity method with calcium cyclamate for chronic carcinogenesis experiments. Tumori 58: 137–142.Google Scholar
  11. 11.
    Toth, B. & Shimizu, H., 1974. 1-Carbamyl-2-phenylhydrazine tumorigenesis in Swiss mice. Morphology of lung adenomas. J. Natl. Cancer Inst. 52: 241–251.Google Scholar
  12. 12.
    Toth, B., Nagel, D., Patil, K., Erickson, J. & Antonson, K., 1978. Tumor induction with the N'-acetyl derivative of 4-hydroxymethylphenylhydrazine, a metabolite of agaritine of Agaricus bisporus. Cancer Res. 38: 177–180.Google Scholar
  13. 13.
    Toth, B., Patil, K., Erickson, J. & Kupper, R., 1979. False morel mushroom Gyromitra esculenta toxin: N-methyl-N-formylhydrazine carcinogenesis in mice. Mycopathologia 68: 121–128.Google Scholar
  14. 14.
    Toth, B., Patil, K. & Jae, H. S., 1981. Carcinogenesis of 4-(hydroxymethyl)benzenediazonium ion (tetrafluoroborate) of Agaricus bisporus. Cancer Res. 41: 2444–2449.Google Scholar
  15. 15.
    Toth, B., Raha, C. R., Wallcave, L. & Nagel, D., 1981. Attempted tumor induction with agaritine in mice. Anticancer Res. 1: 255–258.Google Scholar
  16. 16.
    Toth, B., Nagel, D. & Ross, A., 1982. Gastric tumorigenesis by a single dose of 4-(hydroxymethyl)benzenediazonium ion of Agaricus bisporus. Brit. J. Cancer 46: 417–422.Google Scholar
  17. 17.
    U.S. Department of Agriculture, Statistical Reporting Service, 1982. Crop Reporting Board. Mushrooms, Washington, D.C., Vg 2-1-2:1–4.Google Scholar
  18. 18.
    U.S. Department of Agriculture, Foreign Agricultural Service, 1982. Fresh and processed vegetables, Washington, D.C., FVEG 1–82.Google Scholar
  19. 19.
    Wallcave, L., Nagel, D., Raha, C. R., Jae, H. S., Bronczyk, S., Kupper, R. & Toth, B., 1979. An improved synthesis of agaritine. J. Org. Chem. 44: 3752–3755.Google Scholar

Copyright information

© Dr W. Junk Publishers 1984

Authors and Affiliations

  • B. Toth
    • 1
  • H. Sornson
    • 1
  1. 1.The Eppley Institute for Research in Cancer and Allied DiseasesUniversity of Nebraska Medical CenterOmahaUSA

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