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Mycopathologia

, Volume 84, Issue 2–3, pp 121–131 | Cite as

Biochemical variation of Cryptococcus neoformans

  • Kathryn A. Schmeding
  • Shung C. Jong
  • Rudolph Hugh
Article

Abstract

Ninety-seven strains of Cryptococcus neoformans and C. bacillisporus were examined for 44 biochemical characters and the results were analyzed numerically. One phenon emerged at the 86% level of similarity when strains were clustered according to their M-similarity values. All strains grew in ten carbon sources (D-glucose, D-galactose, arbutin, maltose, sucrose, D-melezitose, D-xylose, D-mannitol, D-glucitol, and meso-inositol), and also grew at 37 ° C and produced urease and phenoloxidase. None of them grew in melibiose, lactose, nor valine, and none reduced nitrate to nitrite. Comparison of selected biochemical characters, creatinine utilization, and serotypes of 49 aberrant strains is presented. Forty-eight of the 97 strains produced the Filobasidiella state either alone or when paired with a strain of compatible mating-type. Filobasidiella neoformans serotypes A and D were interfertile with compatible mating-types of F. bacillispora serotypes B and C. The 44 biochemical characters and 4 serotypes did not predict barriers to mating competence. The present study further substantiates that Filobasidiella neoformans and F. bacillispora are one species.

Keywords

Nitrite Lactose Maltose Cryptococcus Neoformans Arbutin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Ankel, H., Ankel, E. & Feingold, D. S., 1966. Biosynthesis of uridine diphosphate D-xylose. III. Uridine diphosphate D-glucose dehydrogenase of Cryptococcus laurentii. Biochemistry 5: 1864–1869.Google Scholar
  2. 2.
    Ankel, H. & Feingold, D. S., 1966. Biosynthesis of uridine diphosphate D-xylose. II. Uridine diphosphate D-glucuronate carboxy-lyase of Cryptococcus laurentii. Biochemistry 5: 182–189.Google Scholar
  3. 3.
    Bennett, J. E., Kwon-Chung, K. J. & Howard, D. H., 1977. Epidemiologic differences among serotypes of Cryptococcus neoformans. Am. J. Epidemiol. 105: 582–586.Google Scholar
  4. 4.
    Bennett, J. E., Kwon-Chung, K. J. & Theodore, T. S., 1978. Biochemical differences between serotypes of Cryptococcus neoformans. Sabouraudia 16: 167–174.Google Scholar
  5. 5.
    Blandamer, A. & Danishefsky, I., 1966. Investigations on the structure of the capsular polysaccharide from Cryptococcus neoformans type B. Biochim. Biophys. Acta 117: 305–313.Google Scholar
  6. 6.
    Bowman, P. I. & Ahearn, D. G., 1978. Ecology of Cryptococcus neoformans in Georgia. In: Proceedings of the IV International Conference on the Mycoses. PAHO Scientific Publication No. 356, pp. 258–268. Washington, D.C.Google Scholar
  7. 7.
    Cohen, A. & Feingold, D. S., 1967. Uridine diphosphate D-xylose. Acceptor xylosyltransferase of Cryptococcus laurentii. Biochemistry 6: 2933–2939.Google Scholar
  8. 8.
    Denton, J. F. & Di Salvo, A. F., 1968. The prevalence of Cryptococcus neoformans in various natural habitats. Sabouraudia 6: 213–217.Google Scholar
  9. 9.
    Evans, E. E., 1949. An immunologic comparison of twelve strains of Cryptococcus neoformans (Torula histolytica). Proc. Soc. Exp. Biol. Med. 71: 644–646.Google Scholar
  10. 10.
    Evans, E. E., 1950. The antigenic composition of Cryptococcus neoformans. I. A serologic classification by means of the capsular and agglutination reactions, J. Immunol. 64: 423–430.Google Scholar
  11. 11.
    Freeman, W. & Weidman, F. D., 1923. Cystic blastomycosis of the cerebral gray matter caused by Torula histolytica Stoddard and Cutler. Arch. Neurol. Psychiatry 9: 589–603.Google Scholar
  12. 12.
    Hopfer, R. L. & Blank, F., 1975. Caffeic acid containing medium for identification of Cryptococcus neoformans. J. Clin. Microbiol. 2: 115–120.Google Scholar
  13. 13.
    Hopkins, J. M. & Land, G. A., 1977. Rapid method for determining nitrate utilization by yeasts. J. Clin. Microbiol. 5: 497–500.Google Scholar
  14. 14.
    Jong, S. C., 1982. Collection of fungi. In: Catalogue of Strains I, 15th ed., pp. 261–540. American Type Culture Collection, Rockville, Maryland.Google Scholar
  15. 15.
    Kocková-Kratochvilová, A., 1973. Comparative taxonomy of the genus Torulopsis Berlese. J. Gen. Microbiol. 79: 239–256.Google Scholar
  16. 16.
    Kocková-Kratochvílová, A., Slavikova, E. & Jensen, V., 1978. Numerical taxonomy of the yeast genus Debaryomyces Lodder and Kreger-van Rij. J. Gen. Microbiol. 104: 257–268.Google Scholar
  17. 17.
    Kwon-Chung, K. J., 1975. A new genus, Filobasidiella, the perfect state of Cryptococcus neoformans. Mycologia 67: 1197–1200.Google Scholar
  18. 18.
    Kwon-Chung, K. J., 1976. A new species of Filobasidiella, the sexual state of Cryptococcus neoformans B and C serotypes. Mycologia 68: 942–946.Google Scholar
  19. 19.
    Kwon-Chung, K. J., Bennett, J. E. & Theodore, T. S., 1978. Cryptococcus bacillisporus sp. nov.: Serotype B-C of Cryptococcus neoformans. Int. J. Syst. Bacteriol. 28: 616–620.Google Scholar
  20. 20.
    Lodder, J., 1970. General Classification of the yeasts. In: The Yeasts, a Taxonomic Study (Lodder, J., ed.), pp. 1–33. North-Holland Publishing Company, Amsterdam.Google Scholar
  21. 21.
    Miyazaki, T., 1961a. Studies on fungal polysaccharides. I. On the isolation and chemical properties of capsular polysaccharide from Cryptococcus neoformans. Chem. Pharm. Bull., Tokyo 9: 715–718.Google Scholar
  22. 22.
    Miyazaki, T., 1961b. Studies on fungal polysaccharides. II. On the componental sugars and partial hydrolysis of the capsular polysaccharide from Cryptococcus neoformans. Chem. Pharm. Bull., Tokyo 9: 826–829.Google Scholar
  23. 23.
    Miyazaki, T., 1961c. Studies on fungal polysaccharides. III. Chemical structure of the capsular polysaccharide from Cryptococcus neoformans. Chem. Pharm. Bull., Tokyo 9: 829–833.Google Scholar
  24. 24.
    Neill, J. M., Catillo, C. G., Smith, R. H. & Kapros, C. E., 1949. Capsular reactions and soluble antigens of Torula histolytica and of Sporotrichum schenckii. J. Exp. Med. 89: 93–106.Google Scholar
  25. 25.
    Phaff, R. H. & Fell, J. W., 1970. Cryptococcus Kutzing emend. Phaff et Spencer. In: The Yeasts, a Taxonomic Study pp. 1088–1145. (Lodder, J., ed.), North-Holland Publishing Company, Amsterdam.Google Scholar
  26. 26.
    Schmeding, K. A., Jong, S. C. & Hugh, R., 1981. Sexual compatibility between serotypes of Filobasidiella neoformans (Cryptococcus neoformans). Current Microbiol. 5: 133–138.Google Scholar
  27. 27.
    Seeliger, H. P. R., 1956. Use of a urease test for the screening and identification of cryptococci. J. Bacteriol. 72: 127–131.Google Scholar
  28. 28.
    Sneath, P. H. A. & Sokal, R. R., 1973. Numerical Taxonomy. W. H. Freeman and Company, San Francisco. 573 pp.Google Scholar
  29. 29.
    Sokal, R. R. & Sneath, P. H. A., 1963. Principles of Numerical Taxonomy. W. H. Freemand and Company, San Francisco. 359 pp.Google Scholar
  30. 30.
    Staib, F., 1962. Cryptococcus neoformans, Guizotia abyssinica (syn. G. oleifera D.C.). Z. Hyg. Infektionsk. 148: 466–475.Google Scholar
  31. 31.
    Staib, F. & Mishra, S. K., 1975. Zur stammspezifischen Virulenz von Cryptococcus neoformans Tierexperimentelle Beobachtungen über zwei C. neoformans —Stämme aus Vogelfäkalien. Zentralbl. Bakteriol. Parasitent. Infektionskr. Hyg., Abt. 1: Orig. Reihe A. 230: 81–85.Google Scholar
  32. 32.
    Swinne-Desgain, D., 1976. Cryptococcus neoformans in the crops of pigeons following its experimental administration. Sabouraudia 14: 313–317.Google Scholar
  33. 33.
    Vogel, R. A., 1966. The indirect fluorescent antibody test for the detection of the antibody in human cryptococcal disease. J. Infect. Dis. 116: 573–580.Google Scholar
  34. 34.
    Walczek, C., 1979. Complex data analysis. FDA-Bylines 9: 251–253.Google Scholar
  35. 35.
    Walter, J. E. & Coffee, E. G., 1968. Distribution and epidemiologic significance of the serotypes of Cryptococcus neoformans. Am. J. Epidemiol. 87: 167–172.Google Scholar
  36. 36.
    Wickerham, L. J., 1951. Taxonomy of Yeasts. U.S. Dep. Agric. Tech. Bull. No. 1029, Washington, D.C. 56 pp.Google Scholar
  37. 37.
    Wickerham, L. J. & Burton, K. A., 1948. Carbon assimilation tests for the classification of yeasts. J. Bacteriol. 56: 363–371.Google Scholar
  38. 38.
    Wilson, D. E., Bennett, J. E. & Bailey, J. W., 1968. Serologic grouping of Cryptococcus neoformans. Proc. Soc. Exp. Biol. Med. 127: 820–823.Google Scholar

Copyright information

© Dr W. Junk Publishers 1984

Authors and Affiliations

  • Kathryn A. Schmeding
    • 1
  • Shung C. Jong
    • 1
  • Rudolph Hugh
    • 2
  1. 1.Mycology DepartmentAmerican Type Culture CollectionRockvilleUSA
  2. 2.Department of MicrobiologyGeorge Washington University School of MedicineWashington, D.C.USA

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