Release of lysosomal enzymes in Candida albicans phagocytosis by rat peritoneal macrophages
- 30 Downloads
Abstract
The present paper reports the in vitro release of lysosomal enzymes in the supernatant of cultures of rat peritoneal macrophages, with the addition of Candida albicans cells. Macrophages were taken from the rat peritoneal cavity 72 hr after non-specific activation with Brain-Heart-Infusion (B.H.I.) broth containing 10% proteose-peptone No. 3. They were then cultured in Parker medium No. 199 (TC 199). After 24 hr a suspension of Candida albicans cells, in a determined concentration, was added to the peritoneal macrophage cultures. At that time, and during pre-determined periods, the following enzymes in the culture supernatants were studied using colorimetric methods: β-glucuronidase, β-galactosidase and acid phosphatase. It is concluded that, under identical conditions, the release of β-galactosidase and acid phosphatase is higher than for β-glucuronidase. The release rate of all three enzymes is the highest at a 6 hr incubation period, after which, a gradual decrease leads the rate down to 50% at 24 hr.
Keywords
Enzyme Release Rate Candida Culture Supernatant Acid PhosphatasePreview
Unable to display preview. Download preview PDF.
References
- 1.Ajello, L., Georg, L. K., Kaplan, W. & Kaufman, L., 1966. Laboratory manual for medical mycology. Section E. Public Health Service Publication No. 994, U.S., pp. 1–24. Government Printing Office, Washington, D.C.Google Scholar
- 2.Axline, Zanvil & Cohn, Z. A., 1970. In vitro induction of lysosomal enzymes by phagocytosis. J. Exp. Med. 131: 1239–1260.Google Scholar
- 3.Cardella, C. J., Davies, P. & Allison, A. C., 1974. Immune complexes induce selective release of lysosomal hydrolases from macrophages. Nature (Lond.) 247: 47–48.Google Scholar
- 4.Cohn, Z. A. & Wiener, E., 1963. Particulate hydrolases of macrophages. J. Exp. Med. 118: 991–1008.Google Scholar
- 5.Conchie, J., Findlay, J. & Levvy, 1959. Biochem. J. 71: 318–319.Google Scholar
- 6.Davies, P., Allison, A.et al., 1974. Asbestos induces selective release of lysosomal enzymes from mononuclear phagocytes. Nature 251: 423–425.Google Scholar
- 7.Davies, P. & Allison, A. C., 1976. The selective release of acid hydrolases. In: Immunobiology of the Macrophage (Nelson, D. S., ed., pp. 435–438. Academic Press, New York.Google Scholar
- 8.Fishman, W. H.et al., 1953. J. Biol. Chem. 200: 89.Google Scholar
- 9.Joseph, M., Dessaint, J. P. & Capron, A., 1978. Characteristics of macrophage cytotoxicity induced by IgE immune complexes. Cell. Immunol. 34: 247–258.Google Scholar
- 10.Mackenzie, D. W. R., 1966. Laboratory investigation of Candida infections. In: Symposium on Candida infections (Wimer, H. I. & Hurley, R.eds.) pp. 26–43. E. & S. Livingstone Ltd., London.Google Scholar
- 11.Metchnikoff, E., 1905. Immunity in Infective Diseases. Cambridge University Press, London.Google Scholar
- 12.Morgan, J. F., Morton, H. J. & Parker, R. C., 1950. Nutrition of animal cells in tissue culture. I. Initial studies on a synthetic medium. Proc. Soc. Exp. Biol. Med. 73: 1–00.Google Scholar
- 13.Pestel, J., Joseph, M. et al., 1979. The β-glucuronidase release from macrophages activated by immune complexes of varying antigen/antibody ratio. Ann. Immunol. (Inst. Pasteur) 130 C: 507–516.Google Scholar
- 14.Suter, E., 1953. Multiplication of tubercle bacilli within mononuclear phagocytes in tissue cultures derived from normal animals vaccinated with BCG. J. Exp. Med. 97: 235–000.Google Scholar
- 15.Szasz, G., 1967. Die Bestimmung der β-glucuronidas. Aktivität in Serum mit p-nitrophenyl-β-glucuronic. Clin. Chim. Acta 15: 275–280.Google Scholar
- 16.Taschdjian, C. L., Burchall, J. & Kozim, P. J., 1960. Rapid identification of Candida albicans by filamentation on serum and serum substitutes. Am. J. Dis Child 99: 212–215.Google Scholar