Thirty-two isolates of Aspergillus flavus were obtained from various sources in Hungary. All isolates were morphologically identified as A. flavus and three atypical variants were confirmed as A. flavus by comparing their DNA with an ex type culture of A. flavus. None of these isolates produced aflatoxins when tested on coconut agar or grown on rice medium and culture extracts examined by thin layer chromatography. Also, none of the isolates converted sterigmatocystin, O-methyl sterigmatocystin, norsolorinic acid, or sodium acetate to aflatoxin. However, 59% of the isolates produced cyclopiazonic acid based on thin layer chromatographic analysis of culture extracts. The isolates that lack the ability to produce both aflatoxin and cyclopiazonic acid are potential candidates for use in bicontrol studies.
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Richard, J.L., Bhatnagar, D., Peterson, S. et al. Assessment of aflatoxin and cyclopiazonic acid production by Aspergillus flavus isolates from Hungary. Mycopathologia 120, 183–188 (1992). https://doi.org/10.1007/BF00436397
- Aspergillus flavus
- cyclopiazonic acid