Molecular and General Genetics MGG

, Volume 196, Issue 3, pp 482–487 | Cite as

Cloning and expression of the activity and immunity genes of colicins B and M on ColBM plasmids

  • Tobias Ölschläger
  • Edgar Schramm
  • Volkmar Braun


The activity and immunity genes for colicins B and M on two conjugative ColBM plasmids, pCl139 and pF166, were cloned into pBR322 and pACYC184, respectively. The colicin regions on both recombinant plasmids were identical with regard to restriction endonuclease sites and the arrangement of the genes. They map close to each other in the order cmi cma cbi cba, where cmi denotes the locus that determines immunity to colicin M, cma the structural gene for colicin M, cbi immunity to colicin B, and cba the structural gene for colicin B. With the use of mutants obtained by insertion of the transposon Tn5, and by translation in minicells, the transcriptional polarity of cma and cba was found to be from right to left. cma and cba code for polypeptides with molecular weights of 27,000 and 58,000, respectively. No evidence of biosynthetic precursors was obtained.


Molecular Weight Polypeptide Restriction Endonuclease Structural Gene Recombinant Plasmid 
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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Tobias Ölschläger
    • 1
  • Edgar Schramm
    • 1
  • Volkmar Braun
    • 1
  1. 1.Mikrobiologie IIUniversität TübingenTubingenFederal Republic of Germany

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