Molecular and General Genetics MGG

, Volume 196, Issue 3, pp 413–420

High frequency mobilization of gram-negative bacterial replicons by the in vitro constructed Tn5-Mob transposon

  • Reinhard Simon
Article
  • 551 Downloads

Summary

A DNA fragment of the broad host range plasmid RP4 carrying the cis-acting DNA recognition site for conjugative DNA transfer between bacterial cells (Mobsite) was cloned into the kanamycin-neomycin resistance transposon Tn5. Using conventrional transposon mutagenesis techniques the new transposon, called Tn5-Mob, can easily be inserted into the host DNA of gram-negative bacteria. A host replicon carrying Tn5-Mob is then mobilizable into any other gram-negative species if the transfer functions of plasmid RP4 are provided in trans. The potential of Tn5-Mob was demonstrated by mobilizing Rhizobium meliloti plasmids as well as the E. coli chromosome at high frequencies.

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Reinhard Simon
    • 1
  1. 1.Fakultät für BiologieUniversität BielefeldBielefeld 1Federal Republic of Germany

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