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Mycopathologia

, Volume 108, Issue 2, pp 117–123 | Cite as

The efficiency of 1M NH4Cl and 2 M NaCl for the isolation of pathogenic Nocardia from soil

  • Aida van Gelderen de Komaid
  • Estela Liliana Durán
Article

Abstract

The present paper reports an improvement to the classical method of the paraffin bait, by the usage of 1 M NH4Cl or 2 M NaCl to eliminate contaminant microflora of soil.

The purpose is to introduce a change in the paraffin bait method in order to reduce time required to isolate pathogenic strains of Nocardia from their natural sources.

For this study three main criteria were used: a) Determination of the inhibitory effect of different concentrations of salts on soil microflora; b) The tolerance of Nocardia brasiliensis, Nocardia asteroides and Nocardia caviae (Nocardia otitidis caviarum) strains to these chemical inhibitors; c) Determination of the efficiency of salts in the isolation of Nocardia from soil when strains are grown on paraffin baits.

Keywords

Paraffin NH4Cl Natural Source Classical Method Main Criterion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Gordon R, Hagan M. A study of some acid-fast actinomycetes from soil with special reference to pathogenicity to animals. Journal of Infectious Diseases 1936; 59: 200–6.Google Scholar
  2. 2.
    Gonzalez Ochoa A, Sandoval M de los A. Aislamiento de Nocardia brasiliensis y asteroides a partir de suelos. Rev Inst Salubr Enferm Trop 1960; 20: 147–151.Google Scholar
  3. 3.
    Kurup P, Sandhu R. Isolation of Nocardia caviae from soil and its pathogenicity for laboratory animals. J Bact 1965; 90: 822–3.Google Scholar
  4. 4.
    Kurup P, Randhawa H, Sandhu R. A survey of Nocardia asteroides, N. caviae and N. brasiliensis occurring in soil in India. Sabouraudia 1968; 6: 260–6.Google Scholar
  5. 5.
    Mc Clung N. Isolation of Nocardia asteroides from soils. Mycologia 1961; 52: 154–6.Google Scholar
  6. 6.
    Mishra S, Randhawa H. Application of paraffin bait technique to the isolation of Nocardia asteroides from clinical specimens. Appl Microb 1969; 18: 686–7.Google Scholar
  7. 7.
    Mishra S, Randhawa H, Sandhu R. 1973. Observations on paraffin baiting as a laboratory diagnostic procedure in nocardiosis. Mycopathol Mycol Appl 1973; 51: 147–57.Google Scholar
  8. 8.
    Singh M, Sandhu R, Randhawa H. Comparison of paraffin baiting and conventional culture techniques for isolation of Nocardia asteroides from sputum. J Clin Microbiol 1978; 25: 176–7.Google Scholar
  9. 9.
    Valero Guillen P, Martin Luengo F. Nocardia in soils of southeastern Spain: abundance, distribution, and chemical characterization. Can J Microbiol 1984; 30: 1088–92.Google Scholar
  10. 10.
    van Gelderen de Komaid A, R de Laborda R, Salim R. Natural occurrence of Nocardia in soil of Tucumán: Physiological characteristics. Mycopathologia 1987; 99: 15–9.Google Scholar
  11. 11.
    van Gelderen de Komaid A. Physiological and pathogenic characteristics of Nocardia brasiliensis isolated from human mycetomas. Mycopathologia. In Press.Google Scholar
  12. 12.
    van Gelderen de Komaid A, R de Laborda R. Nocardia en suelo de Tucumán. Fisiología y Patogenicidad de las cepas. II Parte. In Press.Google Scholar

Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Aida van Gelderen de Komaid
    • 1
  • Estela Liliana Durán
    • 1
  1. 1.Cátedra de Micología, Instituto de Microbiología ‘Dr. Luis C. Verna’, Facultad de Bioquimica, Quimica y FarmaciaUniversidad National de TucumánTucumánRepública Argentina

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