The efficiency of 1M NH4Cl and 2 M NaCl for the isolation of pathogenic Nocardia from soil
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The present paper reports an improvement to the classical method of the paraffin bait, by the usage of 1 M NH4Cl or 2 M NaCl to eliminate contaminant microflora of soil.
The purpose is to introduce a change in the paraffin bait method in order to reduce time required to isolate pathogenic strains of Nocardia from their natural sources.
For this study three main criteria were used: a) Determination of the inhibitory effect of different concentrations of salts on soil microflora; b) The tolerance of Nocardia brasiliensis, Nocardia asteroides and Nocardia caviae (Nocardia otitidis caviarum) strains to these chemical inhibitors; c) Determination of the efficiency of salts in the isolation of Nocardia from soil when strains are grown on paraffin baits.
KeywordsParaffin NH4Cl Natural Source Classical Method Main Criterion
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