Current Genetics

, Volume 15, Issue 2, pp 75–81

A deletion of the PDC1 gene for pyruvate decarboxylase of yeast causes a different phenotype than previously isolated point mutations

  • Ine Schaaff
  • Jeremy B. A. Green
  • Daniel Gozalbo
  • Stefan Hohmann
Original Articles


We deleted most of the pyruvate decarboxylase structural gene PDC1 from the genome of Saccharomyces cerevisiae. Surprisingly, mutants carrying this deletion allele showed a completely different phenotype than previously described point mutations. They were able to ferment glucose and their specific pyruvate decarboxylase activity was only reduced to 45% of the wild type level. Northern blot analysis revealed that a sequence in the yeast genome homologous to PDC1 and formerly designated as a possible pseudogene is expressed and may code for a different but closely related pyruvate decarboxylase. The products of the two PDC genes seem to form hybrid oligomers, however both homooligomers have enzyme activity. Thus, the product of the PDC1 gene is not absolutely neccessary for glucose fermentation in yeast.

Key words

Alcoholic fermentation Deletion mutant Pyruvate decarboxylase Yeast 


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Ine Schaaff
    • 1
  • Jeremy B. A. Green
    • 1
  • Daniel Gozalbo
    • 1
  • Stefan Hohmann
    • 1
  1. 1.Institut für Mikrobiologie, Technische Hochschule DarmstadtDarmstadtGermany
  2. 2.National Institute for Medical ResearchMill HillLondonUK

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