Mechanism of gene conversion in Ascobolus immersus
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Two two-point crosses in coupling alignement were made between intragenic suppressors at the locus b2. The phenotype of the double mutant is distinguishable both from that of the wild type and of the single mutant. Thus one can select all kinds of recombination events.
The two crosses involve either two mutants which do not give postmeiotic segregation but which show opposite dissymetries in the direction of the conversion, or two mutants only one of which gives postmeiotic segregations.
The basic conversion frequencies of either site are not modified by the presence of the other heterozygote site.
Mutant sites in the heterozygous state may have a reciprocal influence on each other's conversion spectrum.
When a mutant does not produce postmeiotic segregations, these are never induced by the presence of another heterozygous site. On the contrary, one heterozygous site may cause a decrease in the frequency of postmeiotic segregations for another site.
Aberrant 4m:4wt segregations were observed only for the site which otherwise gives 5m:3wt and 3m:5wt segregations.
In the cross which associates two mutants only one of which gives postmeiotic segregation, the modification of this dissymetry is effected in the same way regardless of the type of segregation. Among the double site conversion the extent of the modification of the dissymetry of the meiotic segregations is the same as that of the postmeiotic segregations.
The existence of a process which acts equally on meiotic and postmeiotic segregations implies that there is a common initial phase on which the process regulating segregation acts directly.
These data are discussed both in the hypothesis which involves the formation of a 3-stranded region of D.N.A. as the preliminary step in gene conversion and in the hypothesis involving the formation of an heteroduplex D.N.A. region followed by correction. Only the hypothesis implicating an heteroduplex D.N.A. region as the origin of conversion accounts for all the data.
Hybrid D.N.A. can not be regularly formed at a mutant site in one chromatid only.
The correction is induced by the heterozygous site and not inhibited by it.
The correction started at one site may frequently include the other.
In the majority of cases, excision starts at either site but not at both.
The facts are neither compatible with the idea of a polarized process of recognition of the mispairing along the heteroduplex nor with that of polarized excision.
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