Advertisement

Archives of Orthopaedic and Trauma Surgery

, Volume 116, Issue 1–2, pp 37–40 | Cite as

Effects of single high-dose vitamin D3 on fracture healing

An ultrastructural study in healthy guinea pigs
  • S. Ömeroğlu
  • D. Erdogan
  • H. Ömeroğlu
Original Article

Abstract

The benefits of vitamin D3 on fracture healing have been commonly demonstrated in vitamin-D3-depleted animal models. The aim of this study was to examine the effects of a single high dose of vitamin D3 on fracture healing in a healthy animal model, which has not been previously reported. Twenty healthy young adult guinea pigs were randomly divided into two groups as ‘control’ and ‘vitamin D’, and their right tibias were fractured with digital manipulation. Guinea pigs in vitamin D group were injected intramuscularly with 50000 IU/kg of vitamin D3. The animals were killed at 7, 14, 21 and 28 days following fracture. Ultrastructural analysis of the harvested tibias revealed that a single high dose of vitamin D3 stimulated fracture healing. The observed effects at the fracture zone in a healthy animal model included advancement of blood supply, acceleration of synthesis and organization of collagen fibres, acceleration of the proliferation and differentiation of osteoprogenitor cells, and activation of the mineralization of the matrix.

Keywords

Public Health Collagen Animal Model Blood Supply Collagen Fibre 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Alkalay D, Shany S, Dekel S (1989) Serum and bone vitamin D metabolites in elective patients and patients after fracture. J Bone Joint Surg [Br] 71b:85–87Google Scholar
  2. 2.
    Behrman RE, Kliegman RM, Nelson WE, Vaughan VC III (1992) Nelson textbook of pediatrics. W.B. Saunders, PhiladelphiaGoogle Scholar
  3. 3.
    Beresford JN, Gallagher JA, Russell RGG (1986) 1,25-Dihydroxyvitamin D3 and human bone-derived cells in vitro: effects on alkaline phosphatase, type I collagen and proliferation. Endocrinology 119:1776–1785Google Scholar
  4. 4.
    Brighton CT, Hunt RM (1991) Early histological and ultrastructural changes in medullary fracture callus. J Bone Joint Surg [Am] 73a:832–847Google Scholar
  5. 5.
    Brumbaugh PF, Speer DP, Pitt MJ (1982) 1α,25-Dihydroxyvitamin D3; a metabolite of vitamin D that promotes bone repair. Am J Pathol 106:171–179Google Scholar
  6. 6.
    Buckwalter JA, Cruess RL (1991) Healing of the musculoskeletal tissues. In: Rockwood CA Jr, Green DP, Bucholz RW (eds) Rockwood and Green's fractures in adults. J.B. Lippincott, New York, pp 181–222Google Scholar
  7. 7.
    Dekel S, Salama R, Edelstein S (1983) The effect of vitamin D and its metabolites on fracture repair in chicks. Clin Sci 65:429–436Google Scholar
  8. 8.
    Erdoğan D, Çalgüner E, Kadioğlu D, Aky1ldiz N, Atalay N (1991) Ultrastructural study of the bone lesion in chronic otitis media. J Neurol Orthop Med Surg 12:195–198Google Scholar
  9. 9.
    Hiltunen A, Vuorio E, Aro HT (1993) A standardized experimental fracture in the mouse tibia. J Orthop Res 11:305–312Google Scholar
  10. 10.
    Hulth A (1989) Current concepts of fracture healing. Clin Orthop 249:265–284Google Scholar
  11. 11.
    Lidor C, Dekel S, Edelstein S (1987) The metabolism of vitamin D3 during fracture healing in chicks. Endocrinology 120:389–393Google Scholar
  12. 12.
    Lidor C, Dekel S, Hallel T, Edelstein S (1987) Levels of active metabolites of vitamin D3 in the callus of fracture repair in chicks. J Bone Joint Surg [Br] 69b:132–136Google Scholar
  13. 13.
    Lindgren JU, Narechania RG, McBeath AA, Lange TA, DeLuca HF (1981) Effects of 1.25 dihydroxyvitamin D3 and calcitonin on fracture healing in adult rats. Clin Orthop 160:304–308Google Scholar
  14. 14.
    Meller Y, Kestenbaum RS, Mozes M, Mozes G, Yagil R, Shany S (1984) Mineral and endocrine metabolism during fracture healing in dogs. Clin Orthop 187:289–295Google Scholar
  15. 15.
    Meller Y, Kestenbaum RS, Shany S, Zuili I, Yankowitz N, Giat J, Konforti A, Torok G (1984) Parathyroid hormone, calcitonin, and vitamin D metabolites during normal fracture healing in humans; a preliminary report. Clin Orthop 183:238–245Google Scholar
  16. 16.
    Meller Y, Kestenbaum RS, Shany S, Galinsky D, Zuili I, Yankovitch N, Giat J, Conforti A, Torok G (1985) Parathormone, calcitonin, and vitamin D metabolites during normal fracture healing in geriatric patients. Clin Orthop 199:272–279Google Scholar
  17. 17.
    Pike JW (1987) Emerging concepts on the biologic role and mechanism of action of 1,25-dihydroxyvitamin D3. Steroids 49:3–27Google Scholar
  18. 18.
    Raisz LG, Kream BE (1983) Regulation of bone formation. N Engl J Med 309:29–35Google Scholar
  19. 19.
    Stern PH (1990) Vitamin D and bone. Kidney Int 38 (Suppl 29):517–521Google Scholar
  20. 20.
    Walters MR (1992) Newly identified actions of the vitamin D endocrine system. Endocr Rev 13:719–764Google Scholar

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • S. Ömeroğlu
    • 1
    • 3
  • D. Erdogan
    • 1
  • H. Ömeroğlu
    • 2
  1. 1.Faculty of Medicine, Department of Histology and EmbryologyGazi UniversityAnkaraTurkey
  2. 2.Department of Orthopaedic Surgery and TraumatologyAnkara Numune HospitalAnkaraTurkey
  3. 3.AnkaraTurkey

Personalised recommendations