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Formation and stability of linear plasmids in a recombination deficient strain of yeast

Summary

Natural termini from macronuclear DNA of the ciliated protozoans Tetrahymena thermophila and Oxytricha fallax can support telomere formation in yeast. However, plasmids carrying these ciliate termini are modified by the addition of DNA which hybridizes to the synthetic oligonucleotide poly [d(C-A)], a sequence which also hybridizes to terminal restriction fragments from yeast chromosomes but not to Tetrahymena or Oxytricha macronuclear DNAs. Thus, in yeast, the creation of new telomeres on ciliate termini involves the acquisition of yeast-specific terminal sequences presumably by either recombination or non-templated DNA synthesis. The RAD52 gene is required for the majority of yeast mitotic and meiotic recombination events. Moreover, the absence of an active RAD52 gene product results in high rates of chromosome loss. Here we demonstrate that terminal restriction fragments from Tetrahymena macronuclear ribosomal DNA (rDNA) support the formation of modified telomeres in a yeast strain carrying a defect in the RAD52 gene. Moreover, linear plasmids bearing these modified ciliate termini are stably propagated in rad52 cells.

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References

  1. Bergold PJ, Campbell GR, Littau VC, Johnson EM (1983) Cell 32:1287–1299

  2. Blackburn EH, Gall JG (1978) J Mol Biol 120:33–53

  3. Boswell RE, Klobutcher LA, Prescott DM (1982) Proc Natl Acad Sci USA 79:3255–3259

  4. Dani GM, Zakian VA (1983) Proc Natl Acad Sci USA 80:3406–3410

  5. Davis RW, Thomas M, Cameron J, St John TP, Scherer S, Padget RA (1980) Methods Enzymol 65:404–411

  6. Dunn B, Szauter P, Pardue ML, Szostak JW (1984) Cell 39:191–201

  7. Game JC, Zamb TJ, Braun RJ, Resnick M, Roth RM (1980) Genetics 94:51–68

  8. Heumann JM (1976) Nucleic Acids Res 3:3167–3171

  9. Ho KSY (1975) Mutat Res 30:327–334

  10. Holmquist GP, Dancis B (1979) Proc Natl Acad Sci USA 76:4566–4570

  11. Jackson JA, Fink GR (1981) Nature 292:306–311

  12. King BO, Yao MC (1982) Cell 31:177–182

  13. Kiss GB, Amin AA, Pearlman RE (1981) Mol Cell Biol 1:535–543

  14. Klobutcher LA, Swanton MT, Donini P, Prescott DM (1981) Proc Natl Acad Sci USA 78:3015–3019

  15. Lawn RM, Heumann JM, Herrick G, Prescott DM (1978) Cold Spring Harbor Symp Quant Biol 42:483–492

  16. Malone RE, Esposito RE (1980) Proc Natl Acad Sci USA 77:503–507

  17. Mortimer RK, Contopoulou R, Schild D (1981) Proc Natl Acad Sci USA 78:5778–5782

  18. Oka Y, Honjo T (1983) Nucleic Acids Res 11:4325–4333

  19. Oka Y, Shiota S, Nakai S, Nishida Y, Okubo S (1980) Gene 10:301–306

  20. Pluta AF, Dani GM, Spear BB, Zakian VA (1984) Proc Natl Acad Sci USA 81:1475–1479

  21. Pluta AF, Kaine BP, Spear BB (1982) Nucleic Acids Res 10:8145–8154

  22. Prakash S, Prakash L, Burke W, Montelone BA (1980) Genetics 94:31–50

  23. Preer JR Jr, Preer LB (1979) J Protozool 26:14–18

  24. Resnick MA, Martin P (1976) Mol Gen Genet 143:119–129

  25. Shampay J, Szostak JW, Blackburn EH (1984) Nature 310:154–157

  26. Stinchcomb DT, Mann C, Davis RW (1982) J Mol Biol 158:157–179

  27. Szostak JW, Blackburn EH (1982) Cell 29:245–255

  28. Walmsley RM, Szostak JW, Petes TD (1983) Nature 302:84–86

  29. Walmsley RW, Chan CSM, Tye B-K, Petes TD (1984) Nature 310:157–160

  30. Weiffenbach B, Haber JE (1981) Mol Cell Biol 1:522–534

  31. Wild MA, Gall JG (1979) Cell 16:565–573

  32. Zakian VA, Scott JF (1982) Mol Cell Biol 2:221–232

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Correspondence to Virginia A. Zakian.

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Zakian, V.A., Blanton, H.M. & Dani, G.M. Formation and stability of linear plasmids in a recombination deficient strain of yeast. Curr Genet 9, 441–445 (1985). https://doi.org/10.1007/BF00434048

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Key words

  • Telomeres
  • Recombination
  • Yeast