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Nuclear suppressors of the mitochondrial mutation oxi1-V25 in Saccharomyces cerevisiae

I. The phenotypes of some suppressors

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Summary

Ten nuclear suppressors (nam mutations) of the mitochondrial oxi1-V25 ochre mutation are characterized. They restore to some extent the functional form of cytochrome oxidase, as judged by the results of growth tests, cytochrome spectra, cytochrome oxidase activities, and electrophoresis of the products of mitochondrial translation. The nam mutants can suppress some mit mutations mapping in four mitochondrial genes. They act on a number of chain-terminating mit mutations. When grown on glycerol medium some double mutants nam x-V25 show an increased sensitivity to paromomycin, while the growth of others is stimulated by the drug. The nam mutants are probably omnipotent suppressors resulting from mutations in nuclear gene(s) specifying mitoribosomal protein(s).

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References

  1. Chattoo BB, Palmer E, Ono BJ, Sherman F (1979) Genetics 93: 67–79

  2. Claisse ML, Perre-Aubert G, Clavilier L, Slonimski PP (1970) Eur J Biochem 16:430–438

  3. Claisse ML, Spyridakis A, Slonimski PP (1977) In: Bandlow W, Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1977. Genetics and biogenesis of mitochondria. de Gruyter, Berlin, pp 337–344

  4. Claisse ML, Slonimski PP, Johnson J, Mahler HR (1980) Mol Gen Genet 177:375–387

  5. Conde J, Fink G (1976) Proc Natl Acad Sci USA 73:3651–3655

  6. Corruzi G, Tzagaloff A (1979) J Biol Chem 254:9324–9330

  7. Davey PJ, Haslam JM, Linnane AW (1970) Arch Biochem Biophys 136:54–64

  8. Dujardin G, Pajot P, Groudinsky O, Slonimski PP (1980) Mol Gen Genet 179:469–482

  9. Dujardin G, Kruszewska A, Groudinsky O, Pajot P, Slonimski PP (1980a) In: Kroon AM, Saccone C (eds) The organization and expression of mitochondria) genome. North Holland, Amsterdam, pp 157–164

  10. Fox TD (1979) Proc Natl Acad Sci USA 76:6534–6538

  11. Fox TD, Staempfli S (1982) Proc Natl Acad Sci USA 79:1583–1587

  12. Gorini L (1974) In: Nomura M, Tissieres A, Lengyel P (eds) Ribosomes. Cold Spring Harbor Laboratory, New York, pp 791–803

  13. Groudinsky O, Dujardin G, Slonimski PP (1981) Mol Gen Genet 184:493–503

  14. Hawthorne DC, Leupold U (1974) Curr Top Microbiol Immunol 64:1–47

  15. Ishiguro J, Ono B, Masurekar M, McLaughin CS, Sherman F (1981) J Mol Biol 147:391–397

  16. Jacq C, Pajot P, Lazowska J, Dujardin G, Claisse ML, Groudinsky O, de la Salle H, Grandchamp C, Labuesse M, Gargouri A, Guiard B, Spyridakis A, Dreyfus M, Slonimski PP (1982) In: Attardi G, Borst P, Slonimski PP (eds) Mitochondrial genes. Cold Spring Harbor Laboratory, New York, pp 155–183

  17. Kotylak Z, Slonimski PP (1977) In: Bandlow W, Schweyen RJ, Wolf K, Kaudewitz F (eds) Genetics and biogenesis of mitochondria. de Gruyter, Berlin, pp 83–89

  18. Kruszewska A (1982) In: Attardi G, Borst P, Slonimski PP (eds) Mitochondrial genes. Cold Spring Harbor Laboratory, New York, pp 223–226

  19. Kruszewska A, Sionimski PP (1984a) Curr Genet 9:1–10

  20. Kruszewska A, Slonimski PP (1984b) Curr Genet 9:11–29

  21. Kruszewska A, Szcześniak B (1980) Gen Res Camb 35:225–229

  22. Kruszewska A, Szcześniak B, Claisse ML (1980) Curr Genet 2:45–51

  23. Li M, Tzagaloff A, Underbrink-Lyon K, Martin NC (1982) J Biol Chem 257:5921–5928

  24. Masurekar M, Palmer E, Ono B, Wilhelm JM, Sherman F (1981) J Mol Biol 147:381–390

  25. Mironova LN, Provorov NA, Ter-Avanesyan MD, Inge-Vechtomov SG, Smirnov NV, Surguchov AP (1982) Curr Genet 5:149–152

  26. Pajot P, Wambier-Kluppel ML, Kotylak Z, Slonimski PP (1976) In: Bucher T, Neupert W, Sebald W, Werner S (eds) Genetics and biogenesis of chloroplast and mitochondria. North Holland, Amsterdam, pp 443–451

  27. Palmer E, Wilhelm JM, Sherman F (1979) Nature 277:148–149

  28. Pestka S (1971) Annu Rev Microbiol 25:487–562

  29. Polakowska R, Putrament A (1979) Mutat Res 61:207–213

  30. Singh A, Ursic D, Davies J (1979) Nature 277:146–148

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Correspondence to A. Putrament.

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Żolądek, T., Boguta, M. & Putrament, A. Nuclear suppressors of the mitochondrial mutation oxi1-V25 in Saccharomyces cerevisiae . Curr Genet 9, 427–433 (1985). https://doi.org/10.1007/BF00434046

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Key words

  • Yeast
  • Mitochondrial mutations
  • Informational suppressors