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Current Genetics

, Volume 8, Issue 6, pp 467–470 | Cite as

Cloning and identification of a DNA fragment coding for the sup1 gene of Saccharomyces cerevisiae

  • Peter Breining
  • Andrei P. Surguchov
  • Wolfgang Piepersberg
Short Communication

Summary

A plasmid, pYsup1-1, containing a DNA fragment able to suppress the recessive mutant phenotype of the suppressor locus sup1 (allele sup1-ts36) of Saccharomyces cerevisiae was isolated from a bank of yeast chromosomal DNA cloned in cosmid p3030. The complementing gene was localized on a 2.6 kb DNA fragment by further subcloning. Evidence is presented that the cloned DNA segment codes for the sup1 structural gene (chromosome IIR).

Key words

Saccharomyces cerevisiae Cloning Suppressor 

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Peter Breining
    • 1
  • Andrei P. Surguchov
    • 2
  • Wolfgang Piepersberg
    • 1
  1. 1.Lehrstuhl für Mikrobiologie der Universität MünchenMünchen 19Germany
  2. 2.Institute of Experimental Cardiology, USSR Research Centre of CardiologyAcademy of Medical SciencesMoscowUSSR

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