E. coli x S.typhimurium partially diploid hybrids were constructed to investigate the possibility of genetic complementation between the SA and the SB restriction and modification systems of S. typhimurium and the K and B systems of E. coli.
An hsdR K - mutation was complemented in a stable hybrid in which the hsd SA + -hsd SB + -serB + portion of the S. typhimurium chromosome was integrated at a non-homologous locus. By isolating hsd - mutants in that hybrid, it was shown that complementation occured between K and SB, but not between K and SA.
Similarly, in a set of F-prime merodiploids bearing the SA, SB and B systems, complementation was observed between B and SB, but not between B and SA.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Arber, W.: DNA modification and restriction. Progr. Nucl. Ac. Res. Mol. Biol. 14, 1–38 (1974)
Arber, W., Linn, S.: DNA modification and restriction. Ann. Rev. Biochem. 38, 467–500 (1969)
Arber, W., Wauters-Willems, D.: Host specificity of DNA produced by Escherichia coli. XII. The two restriction and modification systems of strain 15T-. Molec. gen. Genet. 108, 203–217 (1970)
Boyer, H. W., Roulland-Dussoix, D.: A complementation analysis of the restriction and modification of DNA in Escherichia coli. J. molec. Biol. 41, 459–472 (1969)
Bullas, L. R., Colson, C.: New host specificity systems in group C Salmonella recognized by P3 a phage with an unusually wide host range. Abstr. Ann. Meet. A.S.M., p. 58 (1974)
Colson, A. M., Colson, C.: Expression of the Escherichia coli K, B and phage P1 DNA host specificities in Salmonella typhimurium. J. gen. Microbiol. 70, 123–128 (1972)
Colson, C., Colson, A. M.: A new Salmonella typhimurium DNA host specificity. J. gen. Microbiol. 69, 345–351 (1971)
Colson, C., Van Pel, A.: DNA restriction and modification systems in Salmonella. I. SA and SB, two Salmonella typhimurium systems determined by genes with a chromosomal location comparable to that of the Escherichia coli hsd genes. Molec. gen. Genet. 129, 325–337 (1974)
Glover, S. W.: Functional analysis of host-specificity mutants in Escherichia coli. Genet. Res. 15, 237–250 (1970)
Guerola, N., Ingraham, J. L., Cerda-Olmedo, E.: Induction of closely linked multiple mutations by nitrosoguanidine. Nature (Lond.) New Biol. 230, 122–125 (1971)
Karunakaran, V., Middleton, R. B.: Transduction of Salmonella typhimurium genetic material by coliphage P1 in S. typhimurium Hfr x Escherichia coli hybrids. Canad. J. Microbiol. 18, 373–374 (1972)
Meselson, M., Yuan, R., Heywood, J.: Restriction and modification of DNA. Ann. Rev. Biochem. 41, 447–466 (1972)
Piekarowicz, A., Glover, S.W.: Host specificity of DNA in Haemophilus influenzae: The two restriction and modification systems in strain Ra. Molec. gen. Genet. 116, 11–25 (1972)
Piekarowicz, A., Kalinowska, J.: Host specificity of DNA in Haemophilus influenzae: Similarity between host-specificity types of Haemophilus influenzae Re and Rf. J. gen. Microbiol. 81, 405–411 (1974)
Piekarowicz, A., Kauc, L., Glover, S. W.: Host specificity of DNA in Haemophilus influenzae: The restriction and modification systems in strains Rb and Rf. J. gen. Microbiol. 81, 391–403 (1974)
Sanderson, K.E.: Linkage map of Salmonella typhimurium, edition IV. Bact. Rev. 36, 558–586 (1972)
Van Pel, A., Colson, C.: Genetic complementation for DNA restriction and modification between Escherichia coli and Salmonella typhimurium. Abstr. Ann. Meet. A.S.M., p. 23 (1974)
Communicated by W. Arber
About this article
Cite this article
Van Pel, A., Colson, C. DNA restriction and modification systems in Salmonella . Molec. Gen. Genet. 135, 51–60 (1974). https://doi.org/10.1007/BF00433901
- Modification System
- Diploid Hybrid
- Genetic Complementation
- Stable Hybrid