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DNA restriction and modification systems in Salmonella

II. Genetic complementation between the K and B systems of Escherichia coli and the Salmonella typhimurium system SB, with the same chromosomal location

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Summary

E. coli x S.typhimurium partially diploid hybrids were constructed to investigate the possibility of genetic complementation between the SA and the SB restriction and modification systems of S. typhimurium and the K and B systems of E. coli.

An hsdR K - mutation was complemented in a stable hybrid in which the hsd SA + -hsd SB + -serB + portion of the S. typhimurium chromosome was integrated at a non-homologous locus. By isolating hsd - mutants in that hybrid, it was shown that complementation occured between K and SB, but not between K and SA.

Similarly, in a set of F-prime merodiploids bearing the SA, SB and B systems, complementation was observed between B and SB, but not between B and SA.

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References

  1. Arber, W.: DNA modification and restriction. Progr. Nucl. Ac. Res. Mol. Biol. 14, 1–38 (1974)

  2. Arber, W., Linn, S.: DNA modification and restriction. Ann. Rev. Biochem. 38, 467–500 (1969)

  3. Arber, W., Wauters-Willems, D.: Host specificity of DNA produced by Escherichia coli. XII. The two restriction and modification systems of strain 15T-. Molec. gen. Genet. 108, 203–217 (1970)

  4. Boyer, H. W., Roulland-Dussoix, D.: A complementation analysis of the restriction and modification of DNA in Escherichia coli. J. molec. Biol. 41, 459–472 (1969)

  5. Bullas, L. R., Colson, C.: New host specificity systems in group C Salmonella recognized by P3 a phage with an unusually wide host range. Abstr. Ann. Meet. A.S.M., p. 58 (1974)

  6. Colson, A. M., Colson, C.: Expression of the Escherichia coli K, B and phage P1 DNA host specificities in Salmonella typhimurium. J. gen. Microbiol. 70, 123–128 (1972)

  7. Colson, C., Colson, A. M.: A new Salmonella typhimurium DNA host specificity. J. gen. Microbiol. 69, 345–351 (1971)

  8. Colson, C., Van Pel, A.: DNA restriction and modification systems in Salmonella. I. SA and SB, two Salmonella typhimurium systems determined by genes with a chromosomal location comparable to that of the Escherichia coli hsd genes. Molec. gen. Genet. 129, 325–337 (1974)

  9. Glover, S. W.: Functional analysis of host-specificity mutants in Escherichia coli. Genet. Res. 15, 237–250 (1970)

  10. Guerola, N., Ingraham, J. L., Cerda-Olmedo, E.: Induction of closely linked multiple mutations by nitrosoguanidine. Nature (Lond.) New Biol. 230, 122–125 (1971)

  11. Karunakaran, V., Middleton, R. B.: Transduction of Salmonella typhimurium genetic material by coliphage P1 in S. typhimurium Hfr x Escherichia coli hybrids. Canad. J. Microbiol. 18, 373–374 (1972)

  12. Meselson, M., Yuan, R., Heywood, J.: Restriction and modification of DNA. Ann. Rev. Biochem. 41, 447–466 (1972)

  13. Piekarowicz, A., Glover, S.W.: Host specificity of DNA in Haemophilus influenzae: The two restriction and modification systems in strain Ra. Molec. gen. Genet. 116, 11–25 (1972)

  14. Piekarowicz, A., Kalinowska, J.: Host specificity of DNA in Haemophilus influenzae: Similarity between host-specificity types of Haemophilus influenzae Re and Rf. J. gen. Microbiol. 81, 405–411 (1974)

  15. Piekarowicz, A., Kauc, L., Glover, S. W.: Host specificity of DNA in Haemophilus influenzae: The restriction and modification systems in strains Rb and Rf. J. gen. Microbiol. 81, 391–403 (1974)

  16. Sanderson, K.E.: Linkage map of Salmonella typhimurium, edition IV. Bact. Rev. 36, 558–586 (1972)

  17. Van Pel, A., Colson, C.: Genetic complementation for DNA restriction and modification between Escherichia coli and Salmonella typhimurium. Abstr. Ann. Meet. A.S.M., p. 23 (1974)

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Communicated by W. Arber

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Van Pel, A., Colson, C. DNA restriction and modification systems in Salmonella . Molec. Gen. Genet. 135, 51–60 (1974). https://doi.org/10.1007/BF00433901

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Keywords

  • Modification System
  • Diploid Hybrid
  • Genetic Complementation
  • Stable Hybrid