Quantification of the carcinogenic effect of polycyclic aromatic hydrocarbons in used engine oil by topical application onto the skin of mice
The purpose of this investigation was to identify the substances mainly responsible for the carcinogenic effect of used engine oil from gasoline engines using topical application as a carcinogen-specific bioassay. This was performed by comparison of the tumorigenic effect of single fractions with that of an unseparated sample of the lubricating oil.
The used engine oil, from gasoline-driven automobiles, investigated provoked local tumors after long-term application to the dorsal skin of mice. The incidence of carcinoma depended on the dose of the oil.
The fraction of the polycyclic aromatic hydrocarbons (PAH) containing more than three rings accounts for about 70% of the total carcinogenicity in the case of crankcase oil. This fraction constitutes only up to 1.14% by weight of the total oil sample.
The content of benzo(a)pyrene (216.8 mg/kg) accounts for 18% of the total carcinogenicity of the used oil.
Regarding the reduced carcinogenicity of the oil sample, which was reconstituted from all fractions, it seems possible that some of the carcinogenic substances were lost due to volatility, with evaporation of the solvents from the oil-fractionation processes.
Regarding the small effect of the PAH-free fraction, as well as the equal carcinogenic effects of the PAH-fraction (containing more than three rings) and the reconstituted oil sample, no hints for a co-carcinogenic activity were obtained.
Key wordsComparison Carcinogenicity Engine oil Fractions
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- Behn U, Meyer J-P, Grimmer G (1980) PAH-Kumulierung im Motorenschmieröl PAH-Emission aus Ottomotoren. Erdöl and Kohle — Erdgas 33:Google Scholar
- Berichte 1/79 des Umweltbundesamtes (1979) Luftqualitätskriterien für ausgewählte polycyclische aromatische Kohlenwasserstoffe. E. Schmidt, Berlin, pp 245–247Google Scholar
- Breslow N (1979) Statistical methods for censored survival data. Environ Health Perspect 32:181–192Google Scholar
- Brune H (1977) Experimental results with percutaneous applications of automobile exhaust condensates in mice. IARC Sci Publ 15:41–47Google Scholar
- Brune H, Habs M, Schmähl D (1978) The tumor-producing effect of automobile exhaust and fractions thereof, II. J Environ Pathol Toxicol 1:737–746Google Scholar
- Grimmer G, Jacob J, Naujack KW (1981a) Profile of the polycyclic aromatic hydrocarbons from lubricating oils — inventory be GCGC/MS — PAH in environmental material, part 1. Z Anal Chem 306:347–355Google Scholar
- Grimmer G, Jacob J, Naujack KW, Dettbarn G (1981b) Profile of the polycyclic aromatic hydrocarbons from used engine oil — inventory by GCGC/MS — PAH in environmental materials, part 2 Z Anal Chem 309:13–19Google Scholar
- Meyer JP, Grimmer G, Misfeld J, Miller K, Heidemeyer P, Janssen O (1977) BMI-DGMK-Projekt 110. Einflüsse der Betriebsart and Betriebszeit des Motors sowie des Motorenschmieröls auf die Emission von polycyclischen aromatischen Kohlenwasserstoffen (PAH) aus Kraftfahrzeugen mit Ottomotoren. Deutsche Gesellschaft fur Mineralölwissenschaft und Kohlechemie eV, HamburgGoogle Scholar
- Misfeld J, Timm J (1978) The tumor-producing effect of automobile exhaust condensate and fractions thereof, III. J Environ Pathol Toxicol 1:747–772Google Scholar
- Kotin P, Falk HL, Thomas M (1954) Aromatic hydrocarbons, II. Presence in particulate phase of gasoline engine exhaust and carcinogenicity. Arch Ind Hyg 9:164–177Google Scholar
- Wynder EL, Hoffmann D (1962) A study of air pollution carcinogenesis, III. Carcinogenic activity of gasoline engine exhaust condensate. Cancer 15:103–108Google Scholar