This paper describes relationships between osmotic fragility (OF) of red blood cells and various hematologic data in 32 male workers exposed to lead and 30 controls. The examination included measurements of OF determined by the coil planet centrifuge system, red blood cell (RBC) count, hematocrit, hemoglobin, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), water of RBC, sodium and potassium of RBC, blood and urine lead, urine coproporphyrin, and urine δ-aminolevulinic acid. The results were:
OF was lower in lead workers at all three hemolytic points compared with the controls (P < 0.05).
A difference in hemolysis pattern between the two groups was observed (P < 0.05).
A close relationship was observed in both groups between OF and MCV (P < 0.05), although the results were contrasting. Also a close relationship was observed in lead workers between OF and hematocrit, OF and hemoglobin, OF and MCH, and OF and intracellular potassium (P < 0.05) but not in the controls. In the controls a close relationship was found between OF and RBC (P < 0.05) but this was not observed in the lead workers. No significant relationship was observed in either group between OF and MCHC, OF and intracellular sodium, and OF and intracellular water.
No difference in RBC, hematocrit, hemoglobin, MCV, MCH, MCHC, and intracellular water, sodium and potassium was observed between both groups.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Cooper RA, Jandl JH (1968) Bile salts and cholesterol in the pathogenesis of target cells in obstructive jaundice. J Clin Invest 47:809–822
Griggs RC (1964) Lead poisoning: Hematologic aspects. Prog Hematol 4:117–137
Horiguchi S, Teramoto K, Nakano H, Shinagawa K, Endo G, Kiyota I (1974) Osmotic fragility test of red blood cells of lead workers by coil planet centrifuge. Osaka City Med J 20:51–53
Horiuchi K, Horiguchi S, Shinagawa K, Takada F, Teramoto K (1968) A polarographic method for the determination of a small amount of lead in biological materials. Osaka City Med J 14:113–118
Ito Y, Weistein MA, Aoki I, Harada R, Kimura E, Nunogaki K (1966) The coil planet centrifuge. Nature 212:985–987
Kageyama K (1979) Effect of incubation on red cell shape and water content as measured by gas-liquid chromatography. Nouv Rev Fr Hématol 21:161–169
Karai I, Fukumoto K, Horiguchi S (1979) Effects of heavy metal ions on osmotic resistance of human red blood cells. Osaka City Med J 25:153–157
Karai I, Fukumoto K, Horiguchi S (1981a) Studies on osmotic fragility of red blood cells determined with coil planet centrifuge for workers occupationally exposed to lead. Int Arch Occup Environ Health 48:273–281
Karai I, Fukumoto K, Horiguchi S (1981b) The effect of lead on intracellular water content of human red blood cells. 1. An experiment in vitro (in Japanese). Jpn J Ind Health 23:305–307
Karai I, Fukumoto K, Horiguchi S (1982a) Alterations of lipids of the erythrocyte membranes in workers exposed to lead. Int Arch Occup Environ Health (in press)
Karai I, Fukumoto K, Horiguchi S (1982b) The mechanism of an increased osmotic resistance of red blood cells in workers exposed to lead. Br J Ind Med (in press)
Sano S, Granick S (1961) Mitochondrial coproporphyrinogen oxidase and protoporphyrin formation. J Biol Chem 236:1173–1180
Tomokuni K, Ogata M (1972) Simple method for determination of urinary δ-aminolevulinic acid as index of lead exposure. Clin Chem 18:1534–1536
Yamada O (1977) Altered patterns of hemolysis band in hepatobiliary disorders, and their diagnostic significance. Kawasaki Med J 3:111–121
This work was supported in part by Grant-in-Aid for Encouragement of Young Scientists (1981) from the Ministry of Education, Science and Culture of Japan
About this article
Cite this article
Karai, I., Fukumoto, K. & Horiguchi, S. Relationships between osmotic fragility of red blood cells and various hematologic data in workers exposed to lead. Int. Arch Occup Environ Heath 50, 17–24 (1982). https://doi.org/10.1007/BF00432490
- Erythrocyte osmotic fragility
- Lead poisoning
- Erythrocyte water