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Effects of chronic administration of tobacco smoke to mice: Behavioral and metabolic measures

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Abstract

Tobacco smoke was administered to male and female mice of four inbred strains and two lines selectively bred for high activity (HA) or low activity (LA) in an open field. Administration occurred during 14 daily 10-min pretreatment sessions in a box filled with smoke from a nonfiltered cigarette (2 mg nicotine/ cigarette; average density, 750 ppm carbon monoxide). When open-field activity was subsequently measured in the absence or presence of smoke (average density, 150 ppm carbon monoxide), pretreated mice had significantly lower activity scores than controls. Comparisons of open-field activity scores under smoke-present and smoke-absent conditions revealed that effects of this acute exposure were dependent upon genotype: C3H/2Ibg activity was almost tripled in the presence of smoke; DBA/2Ibg activity was increased; but HA, LA, and C57BL/6Ibg activity scores were depressed. As measured by open-field activity, development of tolerance to the effects of acute exposure to tobacco smoke after chronic pretreatment was also genotype-dependent.

Significant genotypic differences were found for nicotine remaining in liver, brain, and blood samples when mice were sacrificed 2.5 or 5 min after a weight-specific injection of radiolabeled nicotine. Tissue nicotine levels were also related to sex, time after injection, and pretreatment interactions with genotype. Strong positive correlations were found between the measures of brain and liver levels at 5 min after injection and the behavioral measure of open-field activity under the smoke-present condition.

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References

  1. Bättig, K.: Differential effects of nicotine and tobacco smoke alkaloids on swimming endurance in the rat. Psychopharmacologia (Berl.) 18, 300–304 (1970)

  2. Beven, J. L.: Inhalation toxicity studies on cigarette smoke. I. A versatile exposure system for inhalation toxicity studies on cigarette smoke. Toxicology 6, 189–196 (1976)

  3. Binns, R.: Inhalation toxicity studies on cigarette smoke. IV. Expression of the dose of smoke particulate material applied to the lungs of experimental animals. Toxicology 7, 189–195 (1977)

  4. Binns, R., Beven, J. L., Wilton, L. V., Lugton, W. G. D.: Inhalation toxicity studies on cigarette smoke. II. Tobacco smoke inhalation dosimetry studies on small laboratory animals. Toxicology 6, 197–206 (1976a)

  5. Binns, R., Beven, J. L., Wilton, L. V., Lugton, W. G. D.: Inhalation toxicity studies on cigarette smoke. III. Tobacco smoke inhalation dosimetry study on rats. Toxicology 6, 207–217 (1976b)

  6. Bovet, D., Bovet-Nitti, F., Oliverio, A.: Action of nicotine on spontaneous and acquired behavior in rats and mice. III. Behavioral and genetic aspects of nicotine and smoke. Ann. N. Y. Acad. Sci. 142, 261–267 (1967)

  7. DeFries, J. C., Wilson, J. R., McClearn, G. E.: Open-field behavior in mice: Selection response and situational generality. Behav. Genet. 1, 195–211 (1970)

  8. Garg, M.: The effect of nicotine on rearing in two strains of rats. Life Sci. 7, 421–429 (1968)

  9. Garg, M.: Variation in effects of nicotine in four strains of rats. Psychopharmacologia (Berl.) 14, 432–438 (1969a)

  10. Garg, M.: The effects of some central nervous system stimulant and depressant drugs on rearing activity in rats. Psychopharmacologia (Berl.) 14, 150–156 (1969b)

  11. Hatchell, P. C., Collins, A. C.: Influences of genotype and sex on behavioral tolerance to nicotine in mice. Pharmacol. Biochem. Behav. 6, 25–30 (1977)

  12. Hatchell, P. C., Collins, A. C.: The influence of genotype and sex on nicotine metabolism, neural concentration of nicotine, and behavioral sensitivity to nicotine. Psychopharmacology (in press, 1980)

  13. Henry, C. T.: Personal communication (1978)

  14. Hrubes, V., Bättig, K.: Effect of inhaled cigarette smoke on swimming endurance in the rat. Arch. Environ. Health 21, 20–24 (1970)

  15. Hubbard, J. E., Gord, R. S.: Tolerance development to the arousal effects of nicotine. Pharmacol. Biochem. Behav. 3, 471–476 (1975)

  16. Larson, P. S., Silvette, H.: Tobacco: Experimental and clinical studies. Supplement III. Baltimore: Williams and Wilkins 1975

  17. Morrison, C. F., Stephenson, J. A.: The occurrence of tolerance to a central depressant effect of nicotine. Br. J. Pharmacol. 45, 151–156 (1972)

  18. Rosecrans, J. A.: Effects of nicotine on behavioral arousal and brain 5-hydroxytryptamine function in female rats selected for differences in activity. Europ. J. Pharmacol. 14, 29–37 (1971)

  19. Rosecrans, J. A., Schechter, M. D.: Brain area nicotine levels in male and female rats of two strains. Arch. int. Pharmacodyn. 196, 46–54 (1972)

  20. Schechter, M. D., Rosecrans, J. A.: Behavioral tolerance to an effect of nicotine in the rat. Arch. Int. Pharmacodyn. 195, 52–56 (1972)

  21. Silverman, A. P.: Behavior of rats given a “smoking dose” of nicotine. Anim. Behav. 19, 67–74 (1971)

  22. Stålhandske, T.: Effects of increased liver metabolism of nicotine on its uptake, elimination and toxicity in mice. Acta Physiol. Scand. 80, 222–234 (1970)

  23. Stålhandske, T., Slanina, P.: Effect of nicotine treatment on the metabolism of nicotine in the mouse liver in vitro. Acta Pharmacol. Toxicol. 28, 75–80 (1970)

  24. Stolerman, I. P., Bunder, P., Jarvik, M. E.: Nicotine tolerance in rats; role of dose and dose interval. Psychopharmacologia (Berl.) 34, 317–324 (1974)

  25. Stolerman, I. P., Fink, R., Jarvik, M. E.: Acute and chronic tolerance to nicotine measured by activity in rats. Psychopharmacologia (Berl.) 30, 329–342 (1973)

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Baer, D.S., McClearn, G.E. & Wilson, J.R. Effects of chronic administration of tobacco smoke to mice: Behavioral and metabolic measures. Psychopharmacology 67, 131–137 (1980). https://doi.org/10.1007/BF00431967

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Key words

  • Acute exposure
  • Chronic exposure
  • Mouse behavior
  • Nicotine metabolism
  • Open-field activity
  • Strains
  • Tobacco smoke