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Archives of Dermatological Research

, Volume 281, Issue 4, pp 267–272 | Cite as

Study of basement membrane formation in dermal-epidermal recombinants in vitro

  • A. Chamson
  • N. Germain
  • A. Claudy
  • C. Perier
  • J. Frey
Original Contributions

Summary

Two different dermal-epidermal recombinants were prepared in vitro and used to study the synthesis and formation of basement membrane. The first was obtained by culturing keratinocytes on the surface of a collagen lattice populated by fibroblasts. The second was prepared by coculture of both keratinocytes and fibroblasts in a collagen lattice. After 6 weeks of culture, the basal lamina was observed with electron microscopy only if keratinocytes were cultivated on top of the collagen lattice populated by fibroblasts. In the second model, however, type IV collagen, laminin, and pemphigoid bullosa antigen could be detected by immunofluorescence as well as synthesis of type IV collagen in the culture, but no basement membrane was observed by electron microscopy. These data demonstrate that, in vitro, basement membrane formation depends not only on the presence of the macromolecular components but also on the culture conditions.

Key words

Basement membrane Dermal-epidermal recombinants Keratinocyte culture Collagen Dermal equivalent 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • A. Chamson
    • 1
  • N. Germain
    • 1
  • A. Claudy
    • 2
  • C. Perier
    • 1
  • J. Frey
    • 1
  1. 1.Biochemistry DepartmentMedical SchoolSaint-Etienne Cedex 2France
  2. 2.Dermatology DepartmentMedical SchoolSaint-Etienne Cedex 2France

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