Immunogenetics

, Volume 22, Issue 4, pp 377–390 | Cite as

DNA polymorphism of the C2 locus

  • D. R. Bentley
  • R. D. Campbell
  • S. J. Cross
Article

Abstract

The extent of the C2 locus in the HLA class III region has been determined by Southern blotting techniques and by DNA sequence analysis. The gene is 18 kb in length and therefore provides a marked contrast to the adjacent factor B gene of 6 kb. A novel restriction fragment length polymorphism (RFLP) has been identified using the endonuclease Sst I and a genomic probe derived from the 5′ region of the C2 gene. Four variants have been detected in a sample of unrelated individuals with haplotypes carrying the C2C allele. Further analysis using C2 and factor B cDNA probes has determined the relationship between this and the other RFLPs previously identified in this region of the genome. Together, the three polymorphisms identified so far make the subdivision of previously indistinguishable haplotypes possible. They therefore constitute a series of markers which increase the resolution of genetic variation in the C2 locus and they may be important in studies of diseases associated with this region of the major histocompatibility complex.

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • D. R. Bentley
    • 1
  • R. D. Campbell
    • 1
  • S. J. Cross
    • 2
  1. 1.Department of BiochemistryMRC Immunochemistry UnitOxfordUK
  2. 2.Genetics LaboratoryDepartment of BiochemistryOxfordUK

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