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Virchows Archiv A

, Volume 392, Issue 2, pp 199–216 | Cite as

Ultrastructure of the normal human aortic media

  • Koert P. Dingemans
  • Norien Jansen
  • Anton E. Becker
Original Articles

Summary

The ultrastructural organization of the adult human aortic media was studied utilizing aortic biopsies from 14 patients, ranging in age from 28 to 67, who underwent cardiac surgery. Apart from solid elastic elements the tissue spaces contained a vast amount of ill-defined thin streaks of elasin, an observation much facilitated by utilizing a selective elastin staining technique. In favorable sections, these streaks were found to be continuous with the solid elastic laminae. Furthermore, most medial smooth muscle cells were in close contact with the thin streaks, but almost none directly with the elastic laminae. The smooth muscle cells had also virtually no connection with collagen fibers. These observations are in contrast with the organization of elastin and with cell-to-stroma connections in the more extensively studied rodent and porcine aortas; they bring into question the role of the smooth muscle cells in the regulation of the viscoelastic properties of the human aortic wall. Other findings were: large number of nexuses connecting the smooth muscle cells, a very small degree of smooth muscle cell degeneration, and the presence of flocculent, fine-granular material investing all formed elements, but especially associated with the thin streaks of elastin.

Key words

Aortic media Human Ultrastructure Elastin Smooth muscle cell 

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References

  1. Bierring F, Kobayasi T (1963) Electron microscopy of the normal rabbit aorta. Acta Pathol Microbiol Scand 57:154–168Google Scholar
  2. Clark JM, Glagov S (1979) Structural integration of the arterial wall. I. Relationships and attachments of medial smooth muscle cells in normally distended and hyperdistended aortas. Lab Invest 40:587–602Google Scholar
  3. Cliff WJ (1967) The aortic tunica media in growing rats studied with the electron microscope. Lab Invest 17:599–615Google Scholar
  4. Cliff WJ (1970) The aortic tunica media in aging rats. Exp Mol Pathol 13:172–189Google Scholar
  5. Dingemans KP, Lettinga KP (1976) Modifications to an LKB Pyramitome. J Microsc (Oxford) 108:301–306Google Scholar
  6. Ferrans VJ, Roberts WC (1976) The carcinoid endocardial plaque. An ultrastructural study. Hum Pathol 7:387–409Google Scholar
  7. Gabella G (1972) The arrangement of sarcoplasmic reticulum in smooth muscle. Experientia 28:948–949Google Scholar
  8. Gabella G (1975) Hypertrophy of intestinal smooth muscle. Cell Tissue Res 163:199–214Google Scholar
  9. Gabella G (1976) Structural changes in smooth muscle cells during isotonic contraction. Cell Tissue Res 170:187–201Google Scholar
  10. Gabella G (1977) Arrangement of smooth muscle cells and intramuscular septa in the taenia coli. Cell Tissue Res 184:195–212Google Scholar
  11. Gerrity RG, Cliff WJ (1975) The aortic tunica media of the developing rat. I. Quantitative stereologic and biochemical analysis. Lab Invest 32:585–600Google Scholar
  12. Haust MD (1965) Fine fibrils of extracellular space (microfibrils). Their structure and role in connective tissue organization. Am J Pathol 47:113–1137Google Scholar
  13. Haust MD, More RK, Bencosme SA, Balis JU (1965) Elastogenesis in human aorta: an electron microscopic study. Exp Mol Pathol 4:508–524Google Scholar
  14. Henderson RM, Duchon G, Daniel EE (1971) Cell contacts in duodenal smooth muscle layers. Am J Physiol 221:564–574Google Scholar
  15. Herr JC (1976) Reflexive gap junctions. Gap junctions between processes arising from the same ovarian decidual cell. J Cell Biol 69:495–501Google Scholar
  16. Imai H, Lee SK, Pastori SJ, Thomas WA (1970) Degeneration of arterial smooth muscle cells: ultrastructural study of smooth muscle cell death in control and cholesterol-fed animals. Virchows Arch [Pathol Anat] 350:163–204Google Scholar
  17. Joris I, Majno G (1974) Cellular breakdown within the arterial wall. An ultrastructural study of the coronary artery in young and aging rats. Virchows Arch [Pathol Anat] 364:111–127Google Scholar
  18. Kádár A (1974) The ultrastructure of elastic tissue. Pathol Europ 9:133–146Google Scholar
  19. Kádár A, Gardner DL, Bush V (1972) Glycosaminoglycans in developing chick-embryo aorta revealed by ruthenium red: an electron microscope study. J Pathol 108:275–280Google Scholar
  20. Kajikawa K, Yamaguchi T, Katsuda S, Miwa A (1975) An improved electron stain for elastic fibers using tannic acid. J Electron Microsc 24:287–289Google Scholar
  21. Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol 27:137AGoogle Scholar
  22. Karrer H, Cox J (1961) An electron microscope study of the aorta in young and aging mice. J Ultrastruct Res 5:1–27Google Scholar
  23. Kim KM (1976) Calcification of matrix vesicles in human aortic valve and aortic media. Fed Proc 35:156–162Google Scholar
  24. Knieriem HJ, Hueber R (1970) Quantitative morphologische Untersuchungen an der Aorta des Menschen. Beitr Path Anat 140:280–297Google Scholar
  25. Lane BP (1965) Alterations in the cytologic detail of intestinal smooth muscle cells in various stages of contraction. J Cell Biol 27:199–213Google Scholar
  26. Massmann J, Weidenbach H (1975) Lichtmikroskopische und elektronenoptische Untersuchungen zur spontanen kalzifizierenden Mediasklerose des Kaninchens. Zentralbl Allg Pathol 119:179–188Google Scholar
  27. Morgan AJ (1980) Mineralized deposits in the thoracic aorta of aged rats: ultrastructural and electron probe X-ray microanalysis. Exp Geront 15:563–573Google Scholar
  28. Paule WJ (1963) Electron microscopy of the newborn rat aorta. J Ultrastruct Res 8:219–235Google Scholar
  29. Race GJ (1971) In Atlas of human histology and ultrastructure, Matthews JL, Martin JH (eds). Lea & Febiger PhiladelphiaGoogle Scholar
  30. Riede UN, Staubesand J (1977) A unifying concept for the role of matrix vesicles and lysosomes in the formal pathogenesis of diseases of connective tissues and blood vessels. Beitr Path 160:3–37Google Scholar
  31. Ross R (1973) The leastic fiber. A review. J Histochem Cytochem 21:199–208Google Scholar
  32. Schlatmann TJM, Becker AE (1977) Histologic changes in the normal aging aorta: implications for dissecting aortic aneurysm. Am J Cardiol 39:13–20Google Scholar
  33. Seifert K (1962) Elektronenmikroskopische Untersuchungen der Aorta des Hausschweines. Z Zellforsch 58:331–368Google Scholar
  34. Seifert K (1963) Elektronenmikroskopische Untersuchungen der Aorta des Kaninchens. Z Zellforsch 60:293–312Google Scholar
  35. Simionescu N, Simionescu M (1976) Galloylglucoses of low molecular weights as mordant in electron microscopy. I. Procedure and evidence for mordanting effect. J Cell Biol 70:608–621Google Scholar
  36. Stein I, Eisenberg S, Stein Y (1969) Aging of aortic smooth muscle cells in rats and rabbits: a morphologic and biochemical study. Lab Invest 21:386–397Google Scholar
  37. Theman TE, Silver MD, Haust MD, McLoughlin MJ, Wigle ED, Williams WR (1979) Morphological findings in idiopathic calcification of the ascending aorta and aortic valve affecting a young woman. Histopathology 3:181–190Google Scholar
  38. Thyberg J, Hinek A, Nilsson J, Friberg U (1979) Electron microscopic and cytochemical studies of rat aorta. Intracellular vesicles containing elastin- und collagen-like material. Histochem J 11:1–17Google Scholar
  39. Trillo A, Haust MD (1975) The granulovesicular bodies of the arterial wall. Lab Invest 32:105–110Google Scholar
  40. Veltman E, Backwinkel KP, Themann H, Hauss WH (1975) Elektronmikroskopische Untersuchungen zur Entstehung von “ghost bodies” in Aorten. Virchows Arch [Pathol Anat] 367:281–288Google Scholar
  41. Wight TN, Ross R (1975) Proteoglycans in primate arteries. I. Ultrastructural localization and distribution in the intima. J Cell Biol 67:660–674Google Scholar
  42. Wolinsky H, Glagov S (1967) A lamellar unit of aortic medial structure and function in mammals. Circ Res 20:99–111Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Koert P. Dingemans
    • 1
  • Norien Jansen
    • 1
  • Anton E. Becker
    • 1
  1. 1.Department of PathologyUniversity of Amsterdam, Wilhelmina GasthuisEG AmsterdamThe Netherlands

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