Molecular and General Genetics MGG

, Volume 205, Issue 2, pp 298–304 | Cite as

The recQ gene of Escherichia coli K12: primary structure and evidence for SOS regulation

  • Nobuto Irino
  • Koji Nakayama
  • Hiroaki Nakayama


A 2,695 bp chromosomal segment of Escherichia coli K12 containing the recQ gene was sequenced. Analysis of the sequence revealed an open reading frame thought to represent recQ, with a clockwise direction of transcription relative to the standard genetic map of E. coli K12 and having a coding capacity for a protein of Mr 68,350. The—10 region of the presumptive recQ promoter overlapped the putative terminator for the upstream gene pldA, and was immediately followed by a 15 bp stretch of DNA bearing a strong resemblance to the reported sequences of LexA repressor binding sites. This latter finding suggested the possibility of SOS regulation of recQ gene expression, which was substantiated by experiments with recQ-lacZ fusions.

Key words

Escherichia coli recQ gene Nucleotide sequence SOS regulation 


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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Nobuto Irino
    • 1
  • Koji Nakayama
    • 1
  • Hiroaki Nakayama
    • 1
  1. 1.Department of Microbiology, School of DentistryKyushu UniversityFukuokaJapan

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