Molecular and General Genetics MGG

, Volume 205, Issue 2, pp 260–269 | Cite as

Cloning and complete nucleotide sequence of the Escherichia coli glutamine permease operon (glnHPQ)

  • Tsutomu Nohno
  • Taiichi Saito
  • Jen-shiang Hong
Article

Summary

The glutamine permease operon encoding the high-affinity transport system of glutamine in Escherichia coli could be cloned in one of the mini F plasmids, but not in pBR322 or pACYC184, by selection for restoration of the Gln+ phenotype, the ability to utilize glutamine as a sole carbon source. We determined the nucleotide sequence of the glutamine permease operon, which contains the structural gene of the periplasmic glutamine-binding protein (glnH), an indispensable component of the permease activity. The N-terminal amino acid sequence and the overall amino acid composition of the purified glutamine-binding protein were in good agreement with those predicted from the nucleotide sequence, if the N-terminal 22 amino acid residues were discounted. The latter comprised two Lys residues (nos. 2 and 6) followed by 16 hydrophobic amino acid residues and was assumed to be a signal peptide for transport into the periplasmic space. There were two additional reading frames (glnP and glnQ) downstream of glnH sharing a common promoter. It was concluded that the glnP and glnQ proteins as well as the glnH protein are essential for glutamine permease activity.

Key words

Glutamine permease Glutamine-binding protein glnHPQ operon Escherichia coli DNA sequence 

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Tsutomu Nohno
    • 1
  • Taiichi Saito
    • 1
  • Jen-shiang Hong
    • 2
  1. 1.Department of PharmacologyKawasaki Medical SchoolKurashiki, OkayamaJapan
  2. 2.Department of Cell PhysiologyBoston Biomedical Research InstituteBostonUSA

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