Antonie van Leeuwenhoek

, Volume 60, Issue 3–4, pp 313–323

Determination of the maximum product yield from glucoamylase-producing Aspergillus niger grown in the recycling fermentor

  • Henk W. van Verseveld
  • M. Metwally
  • M. el Sayed
  • M. Osman
  • Jaap M. Schrickx
  • Adriaan H. Stouthamer
Article

DOI: 10.1007/BF00430372

Cite this article as:
van Verseveld, H.W., Metwally, M., el Sayed, M. et al. Antonie van Leeuwenhoek (1991) 60: 313. doi:10.1007/BF00430372

Abstract

Aspergillus niger has been grown in glucose- and maltose-limited recycling cultures to determine the maximum growth yield, the maximum product yield for glucoamylase production, and the maintenance requirements at very slow specific growth rates. Using the linear equation for substrate utilization, and using the experimental data from both recycling experiments, both the maximum growth yield, Yxsm, and the maximum product yield, Ypsm, could be determined. The values estimated were 157 g biomass per mol maltose for Yxsm and 100 g protein per mol maltose for Ypsm. Expressed on a C1-basis these values are 0.52 and 0.36 C-mole per C-mol for respectively Yxsm and Ypsm. The found value for Ypsm is half the value found for alkaline serine protease production in Bacillus lichoniformis, and it can be concluded that formation of extracellular protein is more energy consuming in filamentous fungi than in prokaryotic organisms. Maintenance requirements are no significant factor during growth of Aspergillus niger, and reported maintenance requirements are most probably due to differentiation.

Key words

Aspergillus recycling fermentor fungi protein production glucoamylase 

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Henk W. van Verseveld
    • 1
  • M. Metwally
    • 1
    • 2
  • M. el Sayed
    • 2
  • M. Osman
    • 2
  • Jaap M. Schrickx
    • 1
  • Adriaan H. Stouthamer
    • 1
  1. 1.Biological Laboratories, Department of MicrobiologyVrije UniversiteitAmsterdamThe Netherlands
  2. 2.Faculty of Sciences, Botany DepartmentTanta UniversityTantaEgypt

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