Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Human skeletal muscle debranching enzyme activities with exercise and training

  • 40 Accesses

  • 1 Citations

Abstract

Sixteen healthy male subjects classified as sedentary (8) or active (8) exercised to exhaustion on a bicycle ergometer at a load requiring approximately 70% of their aerobic capacity. Biopsy samples were taken from the vastus lateralis at rest and at fatigue. A 12 week training program increased skeletal muscle debranching enzyme activities twofold. Submaximal and maximal exercise to exhaustion resulted in decreased debranching enzyme activities. The results indicate that debranching enzyme activities correlate highly with total phosphorylase activities after submaximal and maximal work to fatigue.

This is a preview of subscription content, log in to check access.

References

  1. Huijing, F., Obbink, H. J. K., van Creveld, S.: The activity of the debranching enzyme system in leucocytes: A genetic study of glycogen storage disease Type III. Acta genet. (Basel) 18, 128–136 (1968)

  2. Hultman, E.: Studies on muscle metabolism of glycogen and active phosphate in man with special reference to exercise and diet. Scand. J. clin. Lab. Invest. 94, 1–63 (1967)

  3. Karlsson, J.: Lactate and phosphagen concentrations in working muscle of man. Acta physiol. scand. Suppl. 358 (1971)

  4. Larner, J., Schliselfeld, L. H.: Studies on amylo-1, 6-glucosidase. Biochim. biophys. Acta (Amst.) 20, 53–61 (1956)

  5. Piehl, K.: Time course for refilling of glycogen stores in human muscle fibres following exercise-induced glycogen depletion. Acta physiol. scand. (1974) (in press)

  6. Smith, E. E.: Enzymic control of glycogen structure. In: Control of glycogen metabolism, pp. 203–213. London: Academic Press 1968

  7. Steel, R. G. D., Torrie, J. H.: Principles and procedures of statistics. New York: McGraw Hill 1960

  8. Taylor, A. W., Booth, M. A., Rao, S.: Human skeletal muscle phosphorylase activities with exercise and training. Canad. J. physiol. Pharm. 50, 1038–1042 (1972a)

  9. Taylor, A. W., Lappage, R., Rao, S.: Skeletal muscle glycogen stores after submaximal and maximal work. Med. Sci. Sports 3, 75–78 (1971)

  10. Taylor, A. W., Stothart, J., Booth, M. A., Thayer, R., Rao, S.: Human skeletal muscle glycogen branching enzyme activities with exercise and training. Canad. J. Physiol. Pharm. 529, 119–122 (1974)

  11. Taylor, A. W., Thayer, R., Rao, S.: Human skeletal muscle glycogen synthetase activities with exercise and training. Canad. J. physiol. Pharm. 50, 411–415 (1972b)

  12. Taylor, P. M., Whelan, W. J.: Rabbit muscle amylo-1, 6-glucosidase: Properties and evidence of heterogeneity. In: Control of glycogen metabolism, pp. 101–114. London: Academic Press 1968

Download references

Author information

Additional information

Supported in part by Grant DNHW 55-04021 from the Department of National Health and Welfare, Ottawa. We thank Miss Diane Tougas and Miss Nancy Smith for technical assistance. This work was completed while A. W. Taylor was under the tenure of a research associateship from the Department of National Health and Welfare, Ottawa. Present address of M. Booth is Departments of Physiology and Physical Education, Queen's University, Kingston, Ontario.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Taylor, A.W., Stothart, J., Thayer, R. et al. Human skeletal muscle debranching enzyme activities with exercise and training. Europ. J. Appl. Physiol. 33, 327–330 (1974). https://doi.org/10.1007/BF00430240

Download citation

Key words

  • Human Skeletal Muscle
  • Exercise Training
  • Glycogen
  • Debranching Enzyme