Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Niveaux des hydrolases pancréatiques dans le pancréas et l'intestin grêle de deux types de souris obèses présentant un hyperinsulinisme: La souris obèse-hyperglycémique (oh) de Bar Harbor et la souris Néo-Zélandaise (nzo)

Levels of pancreatic hydrolases in pancreas and small bowel of two types of obese mice with hyperinsulinism: The obese-hyperglycemic Bar-Harbor mouse (OH) and the New-Zealand mouse (NZO)

Die Spiegel der pankreatischen Hydrolasen in der Bauchspeicheldrüse und im Dünndarm bei 2 Arten von fetten Mäusen mit Hyperinsulinismus: der fettsüchtig-hyperglykämischen Bar-Harbor-Maus (OH) und der Neuseeland-Maus (NZO)

Summary

The exocrine pancreas of young obese-hyperglycaemic mice from Bar Harbor and of adult New Zealand obese mice were compared with controls from non-obese animals. The potential activities of lipase, amylase, chymotrypsinogen, trypsinogen and ribonuclease were determined in the gland, as well as the activities corresponding to the first four proteins in the small intestine. These assays indicate that in the two congenital obesities considered, the exocrine function of the pancreas is characterized by normal enzymatic levels.

Résumé

Le pancréas exocrine de la souris jeune obèsehyperglycémique de Bar Harbor et celui de la souris obèse Néo-Zélandaise adulte sont comparés à ceux de souris non-obèses témoins en dosant les activités potentielles de la lipase, de l'amylase, du chymotrypsinogène, du trypsinogène et de la ribonucléase dans la glande ainsi que les activités correspondant aux 4 premières protéines dans l'intestin grêle. Il résulte des dosages que ces paramètres du pancréas exocrine sont normaux dans les deux obésités congénitales considérées.

Zusammenfassung

Die Funktion des exokrinen Pankreas wurde bei jungen fettsüchtig-hyperglykämischen Bar-Harbor-Mäusen und bei erwachsenen fettsüchtigen Neuseeland-Mäusen sowie bei nicht-fettsüchtigen Kontrolltieren verglichen. Die potentiellen Aktivitäten von Lipase, Amylase, Chymotrypsinogen, Trypsinogen und Ribonuclease wurden in der Drüse bestimmt, ferner die Aktivitäten der 4 erstgenannten Eiweißkörper im Dünndarm. Aus den Resultaten geht hervor, daß die Funktion des exokrinen Pankreas bei den beiden untersuchten Fettsuchtstypen durch normale Enzymaktivitäten charakterisiert ist.

Abbreviations

DNA-P et RNA-P:

phosphore des acides désoxy- et ribonucléiques

Bibliographie

  1. Alphin, R.S., and T.M. Lin: Effect of feeding and sham feeding on pancreatic secretion of the rat. Amer. J. Physiol. 197, 260–262 (1959).

  2. Ben Abdeljlil, A., J.C. Palla, and P. Desnuelle: Effect of insulin on pancreatic amylase and chymotrypsinogen. Biochem. Biophys. Res. Comm. 18, 71–75 (1965).

  3. Bielschowsky, M., and F. Bielschowsky: The New Zealand strain of obese mice: their response to stilboestrol and to insulin. Aust. J. exp. Biol. med. Sci. 34, 181–198 (1956).

  4. Binder, H.J., T. Herskovic, H.M. Spiro, and R.P. Spencer: Functional and allometric descriptions of the liver and small intestine in genetically obese mice. J. Nutr. 90, 361–363 (1966).

  5. Chey, W.Y., H. Shay, and C.R. Shuman: External secretory function of the pancreas in diabetes mellitus. J. clin. Invest. 40, 1029 (1961).

  6. Christophe, J.: Le syndrome récessif obésité-hyperglycémie de la souris. Ses relations possibles avec le diabète gras humain. Bull. Acad. Méd. Belg. 5, 309–390 (1965).

  7. —, and J. Mayer: Increased circulating insulinlike activity in obese-hyperglycemic mice. Nature (Lond.) 184, 61–62 (1959).

  8. Friedmah, M.H.F., and W.J. Snape: Influence of secretin and insulin on pancreatic secretion in healthy human subjects. Proc. Soc. exp. Biol., N. Y. 70, 280–283 (1949).

  9. Gepts, W., J. Christophe, and J. Mayer: Pancreatic islets in mice with the obese-hyperglycemic syndrome. Lack of effect of carbutamide. Diabetes 9, 63–69 (1960).

  10. Joslin, E.P., H.F. Root, P. White, and A. Marble: The treatment of diabetes, p. 466. 10th ed. Philadelphia: Lea and Febiger 1959.

  11. Mayer, J., and C.Z. Yannoni: Increased intestinal absorption of glucose in three forms of obesity in the mouse. Amer. J. Physiol. 185, 49–53 (1956).

  12. Morgan, C.M., R.L. Sorenson, and A. Lazarow: Further studies of an inhibition of the two antibody immunoassay system. Diabetes 13, 579–584 (1964).

  13. Peters, N., A.P. Dick, C.N. Hales, D.H. Orrell, and M. Sarner: Exocrine and endocrine pancreatic function in diabetes mellitus and chronic pancreatitis. Gut 7, 277–281 (1966).

  14. Pfeffer, R.B., H.E. Stephenson, and J.W. Hinton: The effect of thoracolumbar sympathectomy and vagus resection on pancreatic function in man. Ann. Surg. 136, 585–594 (1952).

  15. Pictet, R., L. Orci, A.E. Gonet, C. Rouiller, and A.E. Renold: Ultrastructural studies of the hyperplastic islets of Langerhans of spiny mice (Acomys Cahirinus) before and during the development of hyperglycemia. Diabetologia 3, 188–211 (1967).

  16. Robberecht, P., A. Vandermeers, M.H. Khayat, J. Rathe and J. Christophe: Synthèse et excrétion des hydrolases pancréatiques chez le rat dont la malnutrition prend fin. Bull. Soc. Chim. biol. 49, 1735–1750 (1967).

  17. Stauffacher, W., A.E. Lambert, D. Vecchio, and A.E. Renold: Measurements of insulin activities in pancreas and serum of mice with spontaneous (“obese” and “New Zealand obese”) and induced (goldthioglucose) obesity and hyperglycemia, with considerations on the pathogenesis of the spontaneous syndrome. Diabetologia 3, 230–237 (1967).

  18. Täljedal, I., et B. Hellman: Morphological characteristics of the epididymal adipose tissue in different types of hereditary obese mice. Path. Microbiol. 26, 149–157 (1963).

  19. Thomas, J.E., et J.O. Crider: Changes in concentration of enzymes in pancreatic juice after giving insulin. Proc. Soc. exp. Biol., N.Y. 64, 27–31 (1947).

  20. Unger, R.H., H. Ketterer, J. Dupre et A.M. Eisentraut: The effects of secretin, pancreozymin, and gastrin on insulin and glucagon secretion in anesthetized dogs. J. clin. Invest. 46, 630–645 (1967).

  21. Vandermeers, A., P. Robberecht, J. Rathe et J. Christophe: Influence d'une carence en lysine sur le contenu en hydrolases du pancréas et de l'intestin grêle du rat. Bull. Soc. Chim. biol. 48, 133–153 (1966).

Download references

Author information

Additional information

Boursier de l'O.C.D. (Belgique).

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Khayat, M.H., Rathé, J., Vandermeers, A. et al. Niveaux des hydrolases pancréatiques dans le pancréas et l'intestin grêle de deux types de souris obèses présentant un hyperinsulinisme: La souris obèse-hyperglycémique (oh) de Bar Harbor et la souris Néo-Zélandaise (nzo). Diabetologia 4, 232–235 (1968). https://doi.org/10.1007/BF00430100

Download citation

Key-words

  • Spontaneous obesity
  • spontaneous diabetes
  • small intestine
  • Bar Harbor obese hyperglycaemic (OH) mice
  • New Zealand obese (NZO) mice
  • endocrine pancreas
  • hyperinsulinism
  • insulin in serum
  • exocrine pancreas
  • lipase
  • amylase
  • chymotrypsinogen and chymotrypsin
  • trypsinogen and trypsin
  • ribonuclease