2,3-Butanedione monoxime and atropine alone or in combination were evaluated for their ability to alleviate the toxicity and to reverse the biochemical changes induced by dichlorvos in the blood of buffalo calves. Treatment with 2,3-butanedione monoxime plus atropine 30 min after oral administration of dichlorvos (160 mg/kg) eliminated the apparent toxic signs within 10–15 min, completely prevented lethality, and reversed the dichlorvos-induced alterations in the concentrations of serum carboxylesterase, total plasma proteins, blood glucose and plasma cholinesterase within 2, 4, 12 and 168 h, respectively. Treatment with either 2,3-butanedione monoxime or atropine alone was less effective but the former was the more potent of the two in counteracting the biochemical effects of dichlorvos. These antidotal studies suggest that 2,3-butanedione monoxime in conjunction with atropine would provide effective therapy against severe dichlorvos intoxication in buffalo.
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Ecobichon, D.J., 1976. Species differences in the reactivation of organophosphate-inhibited plasma esterases by diacetylmonoxime. Canadian Journal of Physiology and Pharmacology, 54, 86–92
Egyed, M.N. and Bendheim, U., 1977. Mass poisoning in chickens caused by consumption of organophosphorus (dichlorvos) contaminated drinking water. Refuah Veterinarith, 34, 107–110
Frankel, S., Reitman, S. and Sonnerwirtha, A.C., 1970. Gradwohl's Clinical Laboratory Methods and Diagnosis, Vol. I, (C.V. Mosby, St. Louis)
Friedman, J., Dison, M., Hanji, V. and Egyed, M.N., 1987. Mass intoxication in pullets caused by an organophosphorus insecticide. Israel Journal of Veterinary Medicine, 43, 215–216
Gupta, R.C., 1984. Acute malathion toxicosis and related enzymatic alterations in Bubalus bubalis: antidotal treatment with atropine, 2-PAM and diazepam. Journal of Toxicology and Environmental Health, 14, 291–303
Hatch, R.C., 1988. Poisons causing nervous stimulation or depression. In: Booth, N.H. and McDonald, L.E. (eds.), Veterinary Pharmacology and Therapeutics, 6th edn., (Panima Publishing Corporation, New Delhi), 1053–1101
Kerr, L.A., Hudson, L.M. and Osborn, R.G., 1987. Organophosphate toxicosis in a Brangus heifer. Veterinary Medicine, 82, 729, 732, 734
Khan, A.A., Coppock, R.W., Schuler, M.M. and Lillie, L.E., 1988. In vitro and in vivo effects of dichlorvos on blood cholinesterase activities of cattle. American Journal of Veterinary Research, 49, 1184–1187
Kimura, Y., Kishi, Y., Nakamura, S., Yoshinari, H., Ohneda, S., Hirai, S., Satoh, M. and Yamanaka, H., 1988. Organic phosphate insecticide poisoning in fattening calves. Journal of the Japan Veterinary Medical Association, 41, 412–415
Malik, J.K. and Summer, K.H., 1982. Toxicity and metabolism of malathion and its impurities in isolated rat hepatocytes: role of glutathione. Toxicology and Applied Pharmacology, 66, 69–76
Malik, J.K., Raina, R. and Srivastava, A.K., 1985. Comparative studies on the circulating esterases in animals. Fifth Annual Conference of Society of Toxicology (India) Abstracts, 31
Matin, M.A. and Siddiqui, R.A., 1982. Modification of the level of acetylcholinesterase activity by two oximes in certain brain regions and peripheral tissues of paraoxon treated rats. Pharmacological Research Communications, 14, 241–246
Mendoza, C.E., Shields, J.B. and Phillips, W.E.J., 1971. Distribution of carboxylesterase activities in different tissues of albino rats. Comparative Biochemistry and Physiology, 40B, 841–854
Moroi, K., Ushiyama, S., Satoh, T. and Kuga, T., 1976. Enzyme induction by repeated administration of tetrachlorvinphos in rats. Toxicology and Applied Pharmacology, 37, 377–386
Murphy, S.D., 1986. Toxic effects of pesticides. In: Klaassen, C.D., Amdur, M.O. and Doull, J. (eds.), Casarett and Doull's Toxicology, 3rd edn., (Macmillan, New York), 519–581
Pritchard, J., 1989. Organophosphate (terbufos) toxicity in dairy cattle. Canadian Veterinary Journal, 30, 179
Raina, R., 1984. Toxicological and biochemical studies of 2,2-dichlorovinyl dimethyl phosphate (dichlorvos) in Bubalus bubalis, (MVSc thesis, Punjab Agricultural University, Ludhiana)
Singh, G., Sidhu, B.S., Sohoo, M.S. and Tiwana, M.S., 1980. In: Singh, A. (ed.), Fodder Crops, 2nd edn., (P.A.U. Press, Ludhiana)
Singh, S.D., 1981. Toxicological and biochemical studies on O,O-dimethyl S-(N-methylcarbamoylmethyl) phosphorodithioate (dimethoate) in Bubalus bubalis. (PhD thesis, Punjab Agricultural University, Ludhiana)
Smith, R.A. and Lewis, D., 1988. A potpourri of pesticide poisonings in Alberta in 1987. Veterinary and Human Toxicology, 30, 118–120
Taylor, P., 1985. Anticholinesterase agents. In: Gilman, A.G., Goodman, L.S., Rall, T.W. and Murad, F. (eds.), Goodman and Gilman's The Pharmacological Basis of Therapeutics, 7th edn., (Macmillan, New York), 110–129
Vadlamudi, V.P., 1974. Biochemico-toxicological studies on organophosphorus insecticides: malathion and sumithion in buffalo species. (PhD thesis, Punjab Agricultural University, Ludhiana)
Voss, G. and Sachsse, K., 1970. Red cell and plasma cholinesterase activities in microsamples of human and animal blood determined simultaneously by a modified acetylthiocholine/DTNB procedure. Toxicology and Applied Pharmacology, 16, 764–772
Wootton, I.D.P., 1964. Microanalysis in Medical Biochemistry, 4th edn., (J. and A. Churchill Ltd., London)
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Raina, R., Malik, J.K. The therapeutic effects of 2,3-butanedione monoxime and atropine in severe dichlorvos intoxication in buffalo calves. Vet Res Commun 15, 319–325 (1991). https://doi.org/10.1007/BF00430038
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